MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4266)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
398 315.1410 314.1337
399 315.1428 314.1355
400 315.1432 314.1359
401 315.1446 314.1373
402 315.1451 314.1378
403 315.1456 314.1383
404 315.1463 314.1390
405 315.1580 314.1507
406 315.1582 314.1509
407 315.1584 314.1511
408 315.1586 314.1513
409 315.1588 314.1515
410 315.1596 314.1523
411 315.1599 314.1526
412 315.1604 314.1531
413 315.1819 314.1746
414 316.2130 315.2057
415 316.2133 315.2060
416 316.2500 315.2427
417 317.0544 316.0471
418 317.0999 316.0926
419 317.0999 316.0926
420 317.1000 316.0927
421 317.1004 316.0931
422 317.1005 316.0932
423 317.1005 316.0932
424 317.1009 316.0936
425 317.1375 316.1302
426 317.1375 316.1302
427 317.1375 316.1302
428 317.1376 316.1303
429 317.1377 316.1304
430 317.1377 316.1304
431 317.1377 316.1304
432 317.1377 316.1304
433 317.1377 316.1304
434 317.1378 316.1305
435 317.1379 316.1306
436 317.1381 316.1308
437 317.1737 316.1664
438 317.1738 316.1665
439 317.1740 316.1667
440 317.1740 316.1667
441 317.1740 316.1667
442 317.1740 316.1667
443 317.1741 316.1668
444 317.1746 316.1673
445 317.1747 316.1674
446 317.1749 316.1676
447 317.1952 316.1879
448 317.2098 316.2025
449 317.2199 316.2126
450 318.1925 317.1852
451 318.1926 317.1853
452 318.1926 317.1853
453 318.1927 317.1854
454 318.1927 317.1854
455 318.1928 317.1855
456 318.2807 317.2734
457 318.2810 317.2737
458 318.2811 317.2738
459 318.2812 317.2739
460 318.3020 317.2947
461 319.0492 318.0419
462 319.0506 318.0433
463 319.0781 318.0708
464 319.1146 318.1073
465 319.1163 318.1090
466 319.1165 318.1092
467 319.1165 318.1092
468 319.1166 318.1093
469 319.1167 318.1094
470 319.1167 318.1094
471 319.1167 318.1094
472 319.1167 318.1094
473 319.1168 318.1095
474 319.1168 318.1095
475 319.1168 318.1095
476 319.1169 318.1096
477 319.1169 318.1096
478 319.1179 318.1106
479 319.1385 318.1312
480 319.1415 318.1342
481 319.1532 318.1459
482 319.1533 318.1460
483 319.1533 318.1460
484 319.1533 318.1460
485 319.1533 318.1460
486 319.1533 318.1460
487 319.1533 318.1460
488 319.1533 318.1460
489 319.1534 318.1461
490 319.1534 318.1461
491 319.1534 318.1461
492 319.1535 318.1462
493 319.1535 318.1462
494 319.1535 318.1462
495 319.1536 318.1463
496 319.1536 318.1463
497 319.1537 318.1464
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Not what you expected? Try the select summary.