MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
298 311.1465 310.1392
299 311.1476 310.1403
300 311.1479 310.1406
301 311.1480 310.1407
302 311.1480 310.1407
303 311.1481 310.1408
304 311.1481 310.1408
305 311.1481 310.1408
306 311.1481 310.1408
307 311.1482 310.1409
308 311.1482 310.1409
309 311.1482 310.1409
310 311.1482 310.1409
311 311.1484 310.1411
312 311.1484 310.1411
313 311.1485 310.1412
314 311.1498 310.1425
315 311.1657 310.1584
316 311.1660 310.1587
317 311.1662 310.1589
318 311.1663 310.1590
319 311.1667 310.1594
320 311.1847 310.1774
321 311.1848 310.1775
322 311.1848 310.1775
323 311.1849 310.1776
324 311.1851 310.1778
325 311.2053 310.1980
326 311.2228 310.2155
327 311.2229 310.2156
328 311.2229 310.2156
329 311.2229 310.2156
330 311.2230 310.2157
331 311.2231 310.2158
332 312.0315 311.0242
333 312.0548 311.0475
334 312.1318 311.1245
335 312.1318 311.1245
336 312.1320 311.1247
337 313.0385 312.0312
338 313.0603 312.0530
339 313.0606 312.0533
340 313.0607 312.0534
341 313.0611 312.0538
342 313.0612 312.0539
343 313.0871 312.0798
344 313.0891 312.0818
345 313.0905 312.0832
346 313.0966 312.0893
347 313.0980 312.0907
348 313.0999 312.0926
349 313.1073 312.1000
350 313.1165 312.1092
351 313.1268 312.1195
352 313.1271 312.1198
353 313.1279 312.1206
354 313.1284 312.1211
355 313.1293 312.1220
356 313.1295 312.1222
357 313.1636 312.1563
358 313.1638 312.1565
359 313.1639 312.1566
360 313.1640 312.1567
361 313.1640 312.1567
362 313.1640 312.1567
363 313.1641 312.1568
364 313.1644 312.1571
365 313.1648 312.1575
366 313.1649 312.1576
367 313.1650 312.1577
368 313.1826 312.1753
369 313.1827 312.1754
370 313.1828 312.1755
371 313.1830 312.1757
372 313.1837 312.1764
373 313.1842 312.1769
374 313.1842 312.1769
375 313.1843 312.1770
376 313.1843 312.1770
377 313.1844 312.1771
378 313.2209 312.2136
379 313.2211 312.2138
380 313.2389 312.2316
381 314.1225 313.1152
382 314.1978 313.1905
383 314.1978 313.1905
384 314.1978 313.1905
385 315.0539 314.0466
386 315.0546 314.0473
387 315.0758 314.0685
388 315.0779 314.0706
389 315.0779 314.0706
390 315.1065 314.0992
391 315.1132 314.1059
392 315.1137 314.1064
393 315.1217 314.1144
394 315.1219 314.1146
395 315.1220 314.1147
396 315.1220 314.1147
397 315.1221 314.1148
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