MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
198 307.1904 306.1831
199 307.1905 306.1832
200 307.1906 306.1833
201 307.2104 306.2031
202 307.2260 306.2187
203 309.0660 308.0587
204 309.0668 308.0595
205 309.0923 308.0850
206 309.0947 308.0874
207 309.0953 308.0880
208 309.0954 308.0881
209 309.0954 308.0881
210 309.0955 308.0882
211 309.0958 308.0885
212 309.0959 308.0886
213 309.0961 308.0888
214 309.1115 308.1042
215 309.1308 308.1235
216 309.1320 308.1247
217 309.1320 308.1247
218 309.1321 308.1248
219 309.1322 308.1249
220 309.1323 308.1250
221 309.1324 308.1251
222 309.1324 308.1251
223 309.1324 308.1251
224 309.1324 308.1251
225 309.1324 308.1251
226 309.1324 308.1251
227 309.1324 308.1251
228 309.1324 308.1251
229 309.1324 308.1251
230 309.1324 308.1251
231 309.1325 308.1252
232 309.1325 308.1252
233 309.1325 308.1252
234 309.1326 308.1253
235 309.1327 308.1254
236 309.1331 308.1258
237 309.1486 308.1413
238 309.1511 308.1438
239 309.1688 308.1615
240 309.1689 308.1616
241 309.1689 308.1616
242 309.1689 308.1616
243 309.1689 308.1616
244 309.1690 308.1617
245 309.1690 308.1617
246 309.1690 308.1617
247 309.1690 308.1617
248 309.1690 308.1617
249 309.1690 308.1617
250 309.1691 308.1618
251 309.1691 308.1618
252 309.1691 308.1618
253 309.1691 308.1618
254 309.1691 308.1618
255 309.1691 308.1618
256 309.1692 308.1619
257 309.1692 308.1619
258 309.1692 308.1619
259 309.1693 308.1620
260 309.1696 308.1623
261 309.1877 308.1804
262 309.1882 308.1809
263 309.1886 308.1813
264 309.1887 308.1814
265 309.2057 308.1984
266 309.2061 308.1988
267 309.2063 308.1990
268 309.2064 308.1991
269 309.2066 308.1993
270 310.0148 309.0075
271 310.1120 309.1047
272 310.1301 309.1228
273 311.0811 310.0738
274 311.0813 310.0740
275 311.0815 310.0742
276 311.0815 310.0742
277 311.0815 310.0742
278 311.0816 310.0743
279 311.0820 310.0747
280 311.0824 310.0751
281 311.0828 310.0755
282 311.0833 310.0760
283 311.0908 310.0835
284 311.0909 310.0836
285 311.0922 310.0849
286 311.0926 310.0853
287 311.1003 310.0930
288 311.1113 310.1040
289 311.1113 310.1040
290 311.1113 310.1040
291 311.1113 310.1040
292 311.1114 310.1041
293 311.1115 310.1042
294 311.1115 310.1042
295 311.1116 310.1043
296 311.1118 310.1045
297 311.1122 310.1049
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