MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
98 305.1375 304.1302
99 305.1375 304.1302
100 305.1375 304.1302
101 305.1375 304.1302
102 305.1375 304.1302
103 305.1375 304.1302
104 305.1376 304.1303
105 305.1376 304.1303
106 305.1376 304.1303
107 305.1376 304.1303
108 305.1376 304.1303
109 305.1376 304.1303
110 305.1376 304.1303
111 305.1376 304.1303
112 305.1376 304.1303
113 305.1376 304.1303
114 305.1377 304.1304
115 305.1377 304.1304
116 305.1377 304.1304
117 305.1586 304.1513
118 305.1588 304.1515
119 305.1738 304.1665
120 305.1739 304.1666
121 305.1740 304.1667
122 305.1740 304.1667
123 305.1740 304.1667
124 305.1740 304.1667
125 305.1740 304.1667
126 305.1741 304.1668
127 305.1741 304.1668
128 305.1741 304.1668
129 305.1741 304.1668
130 305.1741 304.1668
131 305.1741 304.1668
132 305.1742 304.1669
133 305.1742 304.1669
134 305.1744 304.1671
135 305.1745 304.1672
136 305.1746 304.1673
137 305.1748 304.1675
138 305.2102 304.2029
139 305.2104 304.2031
140 306.1608 305.1535
141 306.1929 305.1856
142 307.0102 306.0029
143 307.0789 306.0716
144 307.0792 306.0719
145 307.0965 306.0892
146 307.1141 306.1068
147 307.1160 306.1087
148 307.1160 306.1087
149 307.1163 306.1090
150 307.1164 306.1091
151 307.1164 306.1091
152 307.1164 306.1091
153 307.1165 306.1092
154 307.1166 306.1093
155 307.1166 306.1093
156 307.1166 306.1093
157 307.1166 306.1093
158 307.1167 306.1094
159 307.1167 306.1094
160 307.1168 306.1095
161 307.1168 306.1095
162 307.1168 306.1095
163 307.1169 306.1096
164 307.1523 306.1450
165 307.1530 306.1457
166 307.1531 306.1458
167 307.1532 306.1459
168 307.1532 306.1459
169 307.1532 306.1459
170 307.1532 306.1459
171 307.1532 306.1459
172 307.1532 306.1459
173 307.1532 306.1459
174 307.1533 306.1460
175 307.1533 306.1460
176 307.1533 306.1460
177 307.1534 306.1461
178 307.1534 306.1461
179 307.1534 306.1461
180 307.1534 306.1461
181 307.1535 306.1462
182 307.1536 306.1463
183 307.1537 306.1464
184 307.1897 306.1824
185 307.1899 306.1826
186 307.1899 306.1826
187 307.1899 306.1826
188 307.1900 306.1827
189 307.1900 306.1827
190 307.1900 306.1827
191 307.1900 306.1827
192 307.1901 306.1828
193 307.1901 306.1828
194 307.1901 306.1828
195 307.1901 306.1828
196 307.1902 306.1829
197 307.1902 306.1829
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