MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3975 476.5834 1426.7284 1426.7304 -1.39 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology		 EGLDITLSDIHSK
3843 408.5614 1222.6624 1222.6557 5.44 1 0 1 +1Score > 21 indicates identity
Score > 13 indicates homology		 EDYKSLLLSR
3956 347.1582 1384.6037 1384.6115 -5.64 0 0 4.9 +1Score > 19 indicates identity YMQMLQDVADR + Oxidation (M)
1 300.0849 299.0776
2 300.0924 299.0851
3 300.1515 299.1442
4 300.1820 299.1747
5 300.1820 299.1747
6 300.2550 299.2477
7 301.0600 300.0527
8 301.0688 300.0615
9 301.0968 300.0895
10 301.1048 300.0975
11 301.1053 300.0980
12 301.1056 300.0983
13 301.1059 300.0986
14 301.1059 300.0986
15 301.1059 300.0986
16 301.1161 300.1088
17 301.1235 300.1162
18 301.1279 300.1206
19 301.1287 300.1214
20 301.1420 300.1347
21 301.1421 300.1348
22 301.1422 300.1349
23 301.1423 300.1350
24 301.1423 300.1350
25 301.1423 300.1350
26 301.1423 300.1350
27 301.1423 300.1350
28 301.1423 300.1350
29 301.1424 300.1351
30 301.1424 300.1351
31 301.1424 300.1351
32 301.1425 300.1352
33 301.1425 300.1352
34 301.1425 300.1352
35 301.1425 300.1352
36 301.1425 300.1352
37 301.1425 300.1352
38 301.1426 300.1353
39 301.1426 300.1353
40 301.1426 300.1353
41 301.1427 300.1354
42 301.1427 300.1354
43 301.1427 300.1354
44 301.1428 300.1355
45 301.1430 300.1357
46 301.1437 300.1364
47 301.1512 300.1439
48 301.1781 300.1708
49 301.2211 300.2138
50 302.1974 301.1901
51 302.1978 301.1905
52 302.2341 301.2268
53 302.2341 301.2268
54 302.2342 301.2269
55 303.0758 302.0685
56 303.0832 302.0759
57 303.0837 302.0764
58 303.0844 302.0771
59 303.0851 302.0778
60 303.0852 302.0779
61 303.0855 302.0782
62 303.1027 302.0954
63 303.1215 302.1142
64 303.1216 302.1143
65 303.1216 302.1143
66 303.1217 302.1144
67 303.1217 302.1144
68 303.1217 302.1144
69 303.1217 302.1144
70 303.1218 302.1145
71 303.1218 302.1145
72 303.1218 302.1145
73 303.1218 302.1145
74 303.1219 302.1146
75 303.1219 302.1146
76 303.1219 302.1146
77 303.1220 302.1147
78 303.1581 302.1508
79 303.1583 302.1510
80 303.1584 302.1511
81 303.1793 302.1720
82 304.0663 303.0590
83 305.0987 304.0914
84 305.0991 304.0918
85 305.0995 304.0922
86 305.1001 304.0928
87 305.1002 304.0929
88 305.1006 304.0933
89 305.1008 304.0935
90 305.1009 304.0936
91 305.1010 304.0937
92 305.1010 304.0937
93 305.1011 304.0938
94 305.1014 304.0941
95 305.1372 304.1299
96 305.1374 304.1301
97 305.1374 304.1301
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