MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4087 541.9783 1622.9131
4088 542.3123 1623.9151
4089 814.3794 1626.7442
4090 409.2224 1632.8605
4091 545.6528 1633.9366
4092 546.3300 1635.9682
4093 546.9886 1637.9440
4095 553.6469 1657.9189
4104 556.9471 1667.8195
4109 563.9769 1688.9089
4112 567.5826 1699.7260
4116 571.2667 1710.7783
4117 571.9977 1712.9713
4118 572.3323 1713.9751
4119 872.4949 1742.9752
4121 582.9206 1745.7400
4126 884.9506 1767.8866
4127 888.9355 1775.8564
4130 601.6358 1801.8856
4131 903.3959 1804.7772
4137 916.5027 1830.9908
4138 614.3471 1840.0195
4139 615.1399 1842.3979
4143 620.9948 1859.9626
4146 934.9912 1867.9678
4147 628.3594 1882.0564
4148 628.6937 1883.0593
4149 944.1528 1886.2910
4150 631.6929 1892.0569
4152 635.0027 1901.9863
4153 953.4562 1904.8978
4154 953.5801 1905.1456
4156 642.0345 1923.0817
4159 973.5904 1945.1662
4160 975.5898 1949.1650
4161 651.9781 1952.9125
4162 979.2189 1956.4232
4165 491.0258 1960.0741
4166 491.0258 1960.0741
4167 654.6998 1961.0776
4168 656.0454 1965.1144
4169 985.9675 1969.9204
4170 986.0869 1970.1592
4174 659.6839 1976.0299
4175 661.7022 1982.0848
4176 995.9600 1989.9054
4179 514.2759 2053.0745
4181 515.2892 2057.1277
4182 693.7038 2078.0896
4185 1061.0291 2120.0436
4186 707.6885 2120.0437
4189 730.3517 2188.0333
4190 736.5818 2206.7236
4193 742.7165 2225.1277
4195 744.3625 2230.0657
4196 745.0649 2232.1729
4198 562.0543 2244.1881
4201 563.2719 2249.0585
4202 752.0816 2253.2230
4205 565.8232 2259.2637
4210 775.7173 2324.1301
4211 791.4073 2371.2001
4212 795.7787 2384.3143
4213 796.4313 2386.2721
4216 597.8060 2387.1949
4218 806.0946 2415.2620
4219 811.1375 2430.3907
4223 819.4166 2455.2280
4226 842.1124 2523.3154
4229 647.5681 2586.2433
4230 647.8131 2587.2233
4231 648.3273 2589.2801
4233 873.8645 2618.5717
4234 880.1282 2637.3628
4235 667.0610 2664.2149
4237 896.3917 2686.1533
4239 907.1534 2718.4384
4241 910.1005 2727.2797
4243 927.8237 2780.4493
4244 949.4542 2845.3408
4245 950.4768 2848.4086
4246 722.1309 2884.4945
4247 725.6336 2898.5053
4248 725.8796 2899.4893
4249 977.1592 2928.4558
4250 994.8361 2981.4865
4251 1072.8641 3215.5705
4252 806.6723 3222.6601
4253 1089.8090 3266.4052
4254 1105.1401 3312.3985
4255 1109.1873 3324.5401
4256 1112.5300 3334.5682
4258 836.1413 3340.5361
4260 992.0194 3964.0485
4264 1119.3065 4473.1969
4268 791.4032 4742.3755
4270 791.5716 4743.3859
4271 869.9341 5213.5609
4272 869.9349 5213.5657
4273 925.3207 5545.8805
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