MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3301 509.2394 508.2321
3302 509.2396 508.2323
3303 511.2531 510.2458
3304 511.2544 510.2471
3305 511.2546 510.2473
3306 511.2548 510.2475
3307 513.2709 512.2636
3309 517.1639 516.1566
3310 517.3002 516.2929
3311 522.2227 521.2154
3312 525.2345 524.2272
3313 525.2718 524.2645
3314 525.2913 524.2840
3316 527.1832 526.1759
3317 529.3132 528.3059
3318 529.3487 528.3414
3319 530.2413 529.2340
3321 534.2729 533.2656
3322 535.2532 534.2459
3323 537.2342 536.2269
3327 561.3283 560.3210
3329 567.2444 566.2371
3330 567.2445 566.2372
3331 569.3177 568.3104
3334 581.2604 580.2531
3336 595.2755 594.2682
3337 595.2755 594.2682
3338 596.6327 595.6254
3339 596.9661 595.9588
3341 301.1429 600.2712
3342 602.2807 601.2734
3343 301.6862 601.3578
3344 302.1032 602.1918
3346 305.1672 608.3198
3348 614.3544 613.3471
3349 307.6862 613.3578
3354 311.0731 620.1316
3357 313.1284 624.2422
3358 314.1508 626.2870
3359 314.1511 626.2876
3361 314.6997 627.3848
3365 314.7250 627.4354
3366 315.1296 628.2446
3367 315.2104 628.4062
3371 642.4327 641.4254
3374 643.2612 642.2539
3375 322.1480 642.2814
3379 323.6844 645.3542
3380 647.2859 646.2786
3383 325.6516 649.2886
3384 326.1411 650.2676
3386 655.3447 654.3374
3390 329.1255 656.2364
3391 329.1256 656.2366
3392 657.3708 656.3635
3393 329.6891 657.3636
3395 658.3923 657.3850
3402 334.1799 666.3452
3403 335.2201 668.4256
3404 336.6786 671.3426
3406 336.7086 671.4026
3408 673.4278 672.4205
3409 679.3474 678.3401
3410 341.1837 680.3528
3411 341.6990 681.3834
3412 683.3276 682.3203
3413 343.1411 684.2676
3414 343.2108 684.4070
3415 343.6975 685.3804
3416 688.3911 687.3838
3420 696.3333 695.3260
3422 350.2436 698.4726
3424 351.2212 700.4278
3428 703.7308 702.7235
3429 352.7228 703.4310
3431 355.1384 708.2622
3432 712.3665 711.3592
3433 357.2022 712.3898
3438 360.6727 719.3308
3440 360.7075 719.4004
3442 363.7024 725.3902
3446 730.3405 729.3332
3447 367.6444 733.2742
3448 368.1909 734.3672
3450 368.2076 734.4006
3451 369.1983 736.3820
3456 372.7180 743.4214
3460 375.1340 748.2534
3461 375.1800 748.3454
3462 749.3827 748.3754
3463 376.2229 750.4312
3464 377.2183 752.4220
3467 381.2090 760.4034
3469 383.1701 764.3256
3470 385.1533 768.2920
3472 386.7054 771.3962
3474 388.6901 775.3656
3478 392.2204 782.4262
3479 392.2239 782.4332
3480 785.4915 784.4842
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