MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2698 421.2220 420.2147
2699 421.2220 420.2147
2700 421.2220 420.2147
2701 421.2221 420.2148
2702 421.2222 420.2149
2703 421.2223 420.2150
2704 421.2224 420.2151
2705 421.2224 420.2151
2706 421.2224 420.2151
2707 421.2226 420.2153
2708 421.2227 420.2154
2709 421.2239 420.2166
2710 421.2240 420.2167
2711 423.1352 422.1279
2712 423.1359 422.1286
2713 423.1646 422.1573
2714 423.1648 422.1575
2715 423.1648 422.1575
2716 423.1648 422.1575
2717 423.1649 422.1576
2718 423.1649 422.1576
2719 423.1651 422.1578
2720 423.1652 422.1579
2721 423.1992 422.1919
2722 423.2000 422.1927
2723 423.2001 422.1928
2724 423.2004 422.1931
2725 423.2005 422.1932
2726 423.2012 422.1939
2727 423.2012 422.1939
2728 423.2013 422.1940
2729 423.2013 422.1940
2730 423.2013 422.1940
2731 423.2014 422.1941
2732 423.2014 422.1941
2733 423.2014 422.1941
2734 423.2014 422.1941
2735 423.2014 422.1941
2736 423.2014 422.1941
2737 423.2015 422.1942
2738 423.2018 422.1945
2739 423.2018 422.1945
2740 423.2018 422.1945
2741 423.2018 422.1945
2742 423.2018 422.1945
2743 423.2024 422.1951
2744 423.2030 422.1957
2745 423.2191 422.2118
2746 423.2196 422.2123
2747 423.2196 422.2123
2748 423.2196 422.2123
2749 423.2196 422.2123
2750 423.2197 422.2124
2751 423.2197 422.2124
2752 423.2197 422.2124
2753 423.2198 422.2125
2754 423.2198 422.2125
2755 423.2198 422.2125
2756 423.2198 422.2125
2757 423.2200 422.2127
2758 423.2200 422.2127
2759 423.2214 422.2141
2760 423.2219 422.2146
2761 423.2274 422.2201
2762 423.2378 422.2305
2763 423.2381 422.2308
2764 423.2383 422.2310
2765 423.2910 422.2837
2766 425.1145 424.1072
2767 425.1493 424.1420
2768 425.1512 424.1439
2769 425.1524 424.1451
2770 425.1805 424.1732
2771 425.1807 424.1734
2772 425.1807 424.1734
2773 425.1807 424.1734
2774 425.1808 424.1735
2775 425.1808 424.1735
2776 425.1808 424.1735
2777 425.1809 424.1736
2778 425.1810 424.1737
2779 425.1810 424.1737
2780 425.1812 424.1739
2781 425.1815 424.1742
2782 425.2162 424.2089
2783 425.2163 424.2090
2784 425.2167 424.2094
2785 425.2167 424.2094
2786 425.2167 424.2094
2787 425.2168 424.2095
2788 425.2169 424.2096
2789 425.2169 424.2096
2790 425.2169 424.2096
2791 425.2169 424.2096
2792 425.2171 424.2098
2793 425.2171 424.2098
2794 425.2172 424.2099
2795 425.2172 424.2099
2796 425.2173 424.2100
2797 425.2173 424.2100
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