MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2498 407.2081 406.2008
2499 407.2082 406.2009
2500 407.2239 406.2166
2501 407.2242 406.2169
2502 407.2245 406.2172
2503 407.2247 406.2174
2504 407.2247 406.2174
2505 407.2247 406.2174
2506 407.2247 406.2174
2507 407.2248 406.2175
2508 407.2249 406.2176
2509 407.2249 406.2176
2510 407.2250 406.2177
2511 407.2441 406.2368
2512 408.2263 407.2190
2513 408.2263 407.2190
2514 408.2614 407.2541
2515 409.1489 408.1416
2516 409.1492 408.1419
2517 409.1492 408.1419
2518 409.1494 408.1421
2519 409.1497 408.1424
2520 409.1498 408.1425
2521 409.1646 408.1573
2522 409.1646 408.1573
2523 409.1662 408.1589
2524 409.1855 408.1782
2525 409.1856 408.1783
2526 409.1856 408.1783
2527 409.1857 408.1784
2528 409.1857 408.1784
2529 409.1857 408.1784
2530 409.1858 408.1785
2531 409.1858 408.1785
2532 409.1858 408.1785
2533 409.1858 408.1785
2534 409.1859 408.1786
2535 409.1859 408.1786
2536 409.1859 408.1786
2537 409.1859 408.1786
2538 409.1859 408.1786
2539 409.1859 408.1786
2540 409.1860 408.1787
2541 409.2104 408.2031
2542 409.2220 408.2147
2543 409.2220 408.2147
2544 409.2221 408.2148
2545 409.2221 408.2148
2546 409.2223 408.2150
2547 409.2224 408.2151
2548 409.2226 408.2153
2549 409.2239 408.2166
2550 409.2403 408.2330
2551 409.2403 408.2330
2552 409.2404 408.2331
2553 409.2404 408.2331
2554 409.2405 408.2332
2555 409.2405 408.2332
2556 409.2407 408.2334
2557 409.2419 408.2346
2558 409.2422 408.2349
2559 409.2735 408.2662
2560 410.2436 409.2363
2561 410.2436 409.2363
2562 410.2439 409.2366
2563 411.0999 410.0926
2564 411.1073 410.1000
2565 411.1372 410.1299
2566 411.1644 410.1571
2567 411.1647 410.1574
2568 411.1648 410.1575
2569 411.1649 410.1576
2570 411.1650 410.1577
2571 411.1650 410.1577
2572 411.1651 410.1578
2573 411.1653 410.1580
2574 411.1655 410.1582
2575 411.1661 410.1588
2576 411.2012 410.1939
2577 411.2012 410.1939
2578 411.2013 410.1940
2579 411.2015 410.1942
2580 411.2016 410.1943
2581 411.2016 410.1943
2582 411.2018 410.1945
2583 411.2018 410.1945
2584 411.2018 410.1945
2585 411.2019 410.1946
2586 411.2019 410.1946
2587 411.2020 410.1947
2588 411.2193 410.2120
2589 411.2561 410.2488
2590 413.1136 412.1063
2591 413.1155 412.1082
2592 413.1158 412.1085
2593 413.1521 412.1448
2594 413.1593 412.1520
2595 413.1595 412.1522
2596 413.1660 412.1587
2597 413.1801 412.1728
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