MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4266)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3818 399.1990 1194.5752 1194.5591 13.5 0 5 0.73 +1Score > 23 indicates identity
Score > 16 indicates homology
FVVDNNMLDK + Deamidated (NQ)
4238 904.7955 2711.3647 2711.3113 19.7 0 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
STDTPPDSPQSFTTALPPSSLMAVHK
3519 412.7557 823.4968 823.5028 -7.24 1 5 0.59 +1Score > 15 indicates identity VRSLPPR
3782 567.2698 1132.5250 1132.5223 2.45 1 5 0.93 +1Score > 23 indicates identity
Score > 17 indicates homology
FSADYQKMK + Oxidation (M)
4217 598.0552 2388.1917 2388.2195 -11.6 1 5 0.55 +1Score > 24 indicates identity
Score > 15 indicates homology
GPRACPGQHYAMIVLTYIVAR + Oxidation (M)
3851 413.5436 1237.6090 1237.6302 -17.2 0 5 0.44 +1Score > 23 indicates identity
Score > 14 indicates homology
GLATASEHPLDK
3499 804.3709 803.3636 803.3524 14.0 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology
EYAFMK + Oxidation (M)
4292 889.8608 6221.9747 6222.0536 -12.7 1 5 1.1 +1Score > 18 indicates identity TIAGVFPLVLACVGAAMMLGISSSNNNARYGGYVLAYQFPICVLSINTFMTAGISGTTK + 3 Deamidated (NQ); 2 Oxidation (M)
4025 758.4257 1514.8368 1514.8416 -3.16 1 5 1 +1Score > 21 indicates identity
Score > 17 indicates homology
QLTLSDATLRIER
3894 651.8601 1301.7056 1301.6979 5.92 0 5 0.96 +1Score > 23 indicates identity
Score > 17 indicates homology
LLQAFPDLQTR + Deamidated (NQ)
3870 421.8691 1262.5855 1262.5813 3.33 0 5 0.4 +1Score > 23 indicates identity
Score > 13 indicates homology
EGGDLVPGAMSSK + Oxidation (M)
4128 897.9152 1793.8158 1793.8505 -19.3 0 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology
MNDLTSDLPYEDILR
4283 936.9957 5615.9305 5615.8792 9.14 0 5 0.65 +1Score > 15 indicates identity IPLVMYDRPQINVDIPLTVGSNLGVLVGVMTAVPWVTVMLCGIHDIDAVHK + 3 Deamidated (NQ); 3 Oxidation (M)
3857 624.3258 1246.6370 1246.6306 5.16 0 5 1 +1Score > 24 indicates identity
Score > 17 indicates homology
GDIFQTVPSQR
3753 367.5351 1099.5835 1099.5873 -3.50 0 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology
QQLTPESAVK
4158 484.5367 1934.1177 1934.0811 18.9 1 4 0.38 +1Score > 13 indicates identity NAFRMFVSLLRPELIK + Deamidated (NQ)
3482 394.7133 787.4120 787.4076 5.70 0 4 0.86 +1Score > 25 indicates identity
Score > 16 indicates homology
GGNELLGK + Deamidated (NQ)
3867 631.3521 1260.6896 1260.6727 13.4 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
LRNAWFNIAR + Deamidated (NQ)
3494 401.2287 800.4428 800.4504 -9.48 1 4 0.49 +1Score > 25 indicates identity
Score > 14 indicates homology
GIGNVGRK + Deamidated (NQ)
3942 456.2542 1365.7408 1365.7365 3.16 0 4 1 +1Score > 21 indicates identity
Score > 17 indicates homology
HLQSVIDAQISR
3505 405.2256 808.4366 808.4330 4.46 0 4 1 +1Score > 20 indicates identity
Score > 17 indicates homology
VYALSEK
3961 695.8842 1389.7538 1389.7728 -13.7 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology
GNASVYGNVIKLR
4098 554.5991 1660.7755 1660.8056 -18.2 1 4 0.47 +1Score > 23 indicates identity
Score > 13 indicates homology
EDSFDLTPLSPRER
3866 629.8019 1257.5892 1257.6102 -16.6 1 4 0.45 +1Score > 21 indicates identity
Score > 13 indicates homology
QAKNETWQPR + Deamidated (NQ)
4236 889.1205 2664.3397 2664.3833 -16.4 1 4 0.65 +1Score > 23 indicates identity
Score > 15 indicates homology
DFKVQDIQLATISQMPYLDAVIR + Deamidated (NQ)
3737 544.2939 1086.5732 1086.5557 16.2 0 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
EVQAIQEAAK + Deamidated (NQ)
3884 641.8154 1281.6162 1281.6243 -6.29 1 4 1 +1Score > 24 indicates identity
Score > 17 indicates homology
IAMSMGMLGIRA + 2 Oxidation (M)
3710 525.2692 1048.5238 1048.5375 -13.1 0 4 0.62 +1Score > 24 indicates identity
Score > 14 indicates homology
ASLDIFPMR
3813 594.3275 1186.6404 1186.6393 0.96 1 4 0.98 +1Score > 24 indicates identity
Score > 16 indicates homology
CLLAYHRVR
4132 603.0002 1805.9788 1805.9999 -11.7 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
ILIDHADNKTLGQQLK
3945 685.8496 1369.6846 1369.7024 -12.9 1 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
VGVPTPMNKGANGK + Deamidated (NQ)
3771 560.8246 1119.6346 1119.6136 18.8 1 4 0.56 +1Score > 20 indicates identity
Score > 14 indicates homology
IKTSDGSVSVK
4134 905.9749 1809.9352 1809.9005 19.2 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology
LDSITYCILTMLHSK + Oxidation (M)
3483 394.7187 787.4228 787.4188 5.17 0 4 0.81 +1Score > 26 indicates identity
Score > 15 indicates homology
LSAAAEAR
3608 308.8326 923.4760 923.4865 -11.4 1 4 0.51 +1Score > 22 indicates identity
Score > 13 indicates homology
KFYQPNK
3720 533.2860 1064.5574 1064.5403 16.1 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
FRFNADPAK
4120 437.4420 1745.7389 1745.7275 6.55 1 4 1.4 +1Score > 18 indicates identity SGQAGSRNGGYESATMR + 2 Deamidated (NQ); Oxidation (M)
4215 597.5755 2386.2729 2386.2467 11.0 1 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology
WPLQSPPMERALHAVSEVVPK + Oxidation (M)
3892 649.7909 1297.5672 1297.5431 18.6 0 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
EIMDMGNGEFR
3466 380.2365 758.4584 758.4538 6.17 1 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
IKELEK
3377 323.1919 644.3692 644.3606 13.5 0 4 0.84 +1Score > 24 indicates identity
Score > 15 indicates homology
NAVVSR
4163 653.3120 1956.9142 1956.9139 0.16 1 4 0.49 +1Score > 23 indicates identity
Score > 13 indicates homology
EIIDFFQMEDEKLQR + Deamidated (NQ); Oxidation (M)
3536 423.2294 844.4442 844.4402 4.74 0 4 1 +1Score > 25 indicates identity
Score > 16 indicates homology
IAAEASAGR
4111 425.6881 1698.7233 1698.7485 -14.8 0 4 2.2 +1Score > 19 indicates identity HSAESSELFEYTSGR
3836 609.3251 1216.6356 1216.6299 4.74 1 4 0.85 +1Score > 25 indicates identity
Score > 15 indicates homology
ELNDELLSKR + Deamidated (NQ)
3989 725.8305 1449.6464 1449.6623 -11.0 0 4 1 +1Score > 22 indicates identity
Score > 16 indicates homology
AGFDEEVADIEQK
4266 753.0303 4512.1381 4512.0833 12.1 1 4 1 +1Score > 21 indicates identity
Score > 16 indicates homology
RCLWNGMMLQGVPNAFFALGYLTNASWTMGVDVTALSACR + 2 Deamidated (NQ); 2 Oxidation (M)
3979 715.8350 1429.6554 1429.6806 -17.6 1 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
QPNGRMPAAASAMK + Deamidated (NQ)
3644 483.2461 964.4776 964.4713 6.60 0 3 0.69 +1Score > 23 indicates identity
Score > 14 indicates homology
SSLSDELSK
3776 563.3274 1124.6402 1124.6553 -13.4 0 3 2 +1Score > 19 indicates identity LLLDIAQSPR
4140 463.7591 1851.0073 1850.9989 4.51 0 3 0.49 +1Score > 20 indicates identity
Score > 13 indicates homology
NATLITIGDPNTPPVTVK + Deamidated (NQ)
3573 446.7163 891.4180 891.4086 10.6 0 3 0.55 +1Score > 23 indicates identity
Score > 13 indicates homology
QTSQHYK + Deamidated (NQ)
4035 512.9297 1535.7673 1535.7654 1.24 1 3 0.95 +1Score > 24 indicates identity
Score > 16 indicates homology
SEMWEVTNKAVVK + Oxidation (M)
3810 394.2094 1179.6064 1179.5884 15.2 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
SSGDRNGLFVK + Deamidated (NQ)
4209 773.7125 2318.1157 2318.1252 -4.13 1 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
IPFMRLSTQEIDLQYQYR + 2 Deamidated (NQ); Oxidation (M)
4401 1114.8191 7796.6828 7796.6095 9.40 1 3 1.2 +1Score > 17 indicates identity MKPEPGVSEAGDQPVLAYPPHAPLGQVPNMHPDMRYQPQTHPNPALPLLQNPYMPGGYTSAPPMPNGGAPQGR + 7 Deamidated (NQ); 2 Oxidation (M)
3941 683.8569 1365.6992 1365.7074 -6.00 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
SRYIMTTELPR
3356 312.1805 622.3464 622.3439 4.16 0 3 1.8 +1Score > 18 indicates identity VSVYR
3783 567.2706 1132.5266 1132.5223 3.86 1 3 1 +1Score > 23 indicates identity
Score > 16 indicates homology
FSADYQKMK + Oxidation (M)
4097 831.3946 1660.7746 1660.7879 -7.97 0 3 0.83 +1Score > 23 indicates identity
Score > 15 indicates homology
NMEGAHLVTIEEFR + Oxidation (M)
3690 515.7744 1029.5342 1029.5454 -10.9 1 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
QKQQLLNR + 3 Deamidated (NQ)
3987 483.6023 1447.7851 1447.8035 -12.7 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology
GGLLSLIYNQTLR + Deamidated (NQ)
3965 470.9056 1409.6950 1409.6722 16.2 1 3 1 +1Score > 24 indicates identity
Score > 16 indicates homology
GQATGRMAVDFNK + Oxidation (M)
3990 729.3484 1456.6822 1456.7059 -16.2 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
QYAGNGEQHIALR + Deamidated (NQ)
3949 457.8788 1370.6146 1370.6361 -15.7 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
QRQMQQPDAPR + Deamidated (NQ); Oxidation (M)
4269 949.4827 4742.3771 4742.3539 4.90 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
FLLEAMGEVLGDALTPEILDAWATAYWQLADLMIGREAELYK + Deamidated (NQ); Oxidation (M)
3672 337.8364 1010.4874 1010.4929 -5.46 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
QIPYTMMK
3582 450.7697 899.5248 899.5301 -5.85 1 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
RSGVVGAVR
3609 463.2184 924.4222 924.4188 3.69 1 3 1 +1Score > 21 indicates identity
Score > 15 indicates homology
DEQYKNK + Deamidated (NQ)
3828 401.8651 1202.5735 1202.5713 1.78 1 3 0.95 +1Score > 24 indicates identity
Score > 15 indicates homology
AGEEMPLRER + Oxidation (M)
3840 610.8330 1219.6514 1219.6601 -7.08 1 3 0.85 +1Score > 23 indicates identity
Score > 15 indicates homology
EWLFIQEKK
3382 325.1887 648.3628 648.3595 5.15 0 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
IVFDR
4133 603.6423 1807.9051 1807.9389 -18.7 1 3 0.77 +1Score > 24 indicates identity
Score > 14 indicates homology
VMYGAEKIELSELAQK
4125 589.6588 1765.9546 1765.9727 -10.2 1 3 1 +1Score > 20 indicates identity
Score > 15 indicates homology
DFVGQHVKAADKPIIK + Deamidated (NQ)
3881 428.1975 1281.5707 1281.5911 -15.9 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
MTNYQIDTPAK + Deamidated (NQ)
3930 676.3146 1350.6146 1350.6198 -3.79 1 3 0.63 +1Score > 23 indicates identity
Score > 13 indicates homology
MENVSDKQTQR + Oxidation (M)
3580 449.7921 897.5696 897.5760 -7.03 1 3 1 +1Score > 15 indicates identity IIIIDRR
4034 768.8909 1535.7672 1535.7654 1.23 1 3 0.9 +1Score > 24 indicates identity
Score > 15 indicates homology
SEMWEVTNKAVVK + Oxidation (M)
3528 417.7202 833.4258 833.4104 18.6 1 3 0.93 +1Score > 24 indicates identity
Score > 15 indicates homology
SSQNRSR
4123 883.8994 1765.7842 1765.7642 11.4 0 3 1 +1Score > 22 indicates identity
Score > 15 indicates homology
DAAYGAPSELTEEEER
3951 458.2353 1371.6841 1371.7068 -16.5 1 3 0.69 +1Score > 23 indicates identity
Score > 14 indicates homology
SQLCTKAQLPPK + 2 Deamidated (NQ)
4102 556.6525 1666.9357 1666.9559 -12.1 1 3 2.1 +1Score > 18 indicates identity VAILFFRSTTIPFR
4108 842.9044 1683.7942 1683.7636 18.2 0 3 1 +1Score > 23 indicates identity
Score > 15 indicates homology
MFTNAVMFNPLPQR + 3 Deamidated (NQ); Oxidation (M)
3995 732.8751 1463.7356 1463.7290 4.56 1 3 0.65 +1Score > 24 indicates identity
Score > 13 indicates homology
DAMEGKNTLTGLAK + Oxidation (M)
3695 519.7671 1037.5196 1037.5029 16.1 0 2 0.74 +1Score > 23 indicates identity
Score > 14 indicates homology
LSASSGYPEK
3439 360.6907 719.3668 719.3562 14.8 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
EGSSGKR
4203 753.0729 2256.1969 2256.1613 15.8 0 2 1 +1Score > 22 indicates identity
Score > 15 indicates homology
LYGQCSAALAAVLYVPFLSGR + Deamidated (NQ)
3773 561.7648 1121.5150 1121.5241 -8.04 0 2 0.8 +1Score > 23 indicates identity
Score > 14 indicates homology
EVQDFDELK
3947 685.8966 1369.7786 1369.7565 16.2 1 2 0.88 +1Score > 20 indicates identity
Score > 14 indicates homology
ALQEQVQQGKIK + Deamidated (NQ)
3653 488.7777 975.5408 975.5349 6.10 1 2 0.92 +1Score > 22 indicates identity
Score > 14 indicates homology
ISSVAKDTR
4129 599.3322 1794.9748 1794.9450 16.6 1 2 2.8 +1Score > 19 indicates identity MFPKEALHEQPIALR + Oxidation (M)
3664 497.8269 993.6392 993.6223 17.1 0 2 0.59 +1Score > 13 indicates identity LIAVVPASPK
4363 1098.3813 7681.6182 7681.6523 -4.44 1 2 1.3 +1Score > 16 indicates identity SPAIAPQAAQSQTNEPATPAMLMRIQRPQHSPAAIGQASGPVSSESQDDIMEDVILPEAATPTTHFSQSQVAR + 4 Deamidated (NQ); 2 Oxidation (M)
4099 554.9332 1661.7778 1661.7533 14.7 0 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
NPALDVPQSSQSFNR + 3 Deamidated (NQ)
3890 430.8529 1289.5369 1289.5484 -8.90 0 2 1.4 +1Score > 16 indicates identity SQQQQQQQQR + 4 Deamidated (NQ)
3527 416.7446 831.4746 831.4701 5.42 1 2 0.8 +1Score > 23 indicates identity
Score > 14 indicates homology
KLISENK + Deamidated (NQ)
3959 693.8994 1385.7842 1385.7878 -2.57 1 2 0.8 +1Score > 20 indicates identity
Score > 14 indicates homology
LQQVVKVSESLR + Deamidated (NQ)
4242 923.4483 2767.3231 2767.3164 2.43 0 2 0.73 +1Score > 24 indicates identity
Score > 13 indicates homology
VDSGDGTWSPISPATFKPVFSPMNSK + Oxidation (M)
3353 309.6671 617.3196 617.3133 10.3 0 2 0.91 +1Score > 27 indicates identity
Score > 14 indicates homology
NLSQR + Deamidated (NQ)
4157 483.2523 1928.9801 1929.0094 -15.2 1 2 1 +1Score > 23 indicates identity
Score > 15 indicates homology
NGKNVQNIIDLIISAWK + 4 Deamidated (NQ)
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