MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2398 399.2207 398.2134
2399 399.2216 398.2143
2400 399.2365 398.2292
2401 400.2207 399.2134
2402 401.0910 400.0837
2403 401.1137 400.1064
2404 401.1142 400.1069
2405 401.1498 400.1425
2406 401.1592 400.1519
2407 401.1592 400.1519
2408 401.1594 400.1521
2409 401.1596 400.1523
2410 401.1597 400.1524
2411 401.1601 400.1528
2412 401.1611 400.1538
2413 401.2173 400.2100
2414 401.2176 400.2103
2415 401.2359 400.2286
2416 402.2479 401.2406
2417 402.2481 401.2408
2418 403.1729 402.1656
2419 403.1748 402.1675
2420 403.1750 402.1677
2421 403.1750 402.1677
2422 403.1750 402.1677
2423 403.1750 402.1677
2424 403.1750 402.1677
2425 403.1751 402.1678
2426 403.1752 402.1679
2427 403.1752 402.1679
2428 403.1752 402.1679
2429 403.1754 402.1681
2430 403.2115 402.2042
2431 403.2120 402.2047
2432 403.2318 402.2245
2433 404.2665 403.2592
2434 405.1541 404.1468
2435 405.1542 404.1469
2436 405.1544 404.1471
2437 405.1544 404.1471
2438 405.1550 404.1477
2439 405.1903 404.1830
2440 405.1906 404.1833
2441 405.1906 404.1833
2442 405.1906 404.1833
2443 405.1907 404.1834
2444 405.1908 404.1835
2445 405.1908 404.1835
2446 405.1908 404.1835
2447 405.1909 404.1836
2448 405.1909 404.1836
2449 405.1910 404.1837
2450 405.1911 404.1838
2451 405.1915 404.1842
2452 405.1919 404.1846
2453 405.1922 404.1849
2454 405.1925 404.1852
2455 405.2087 404.2014
2456 405.2099 404.2026
2457 405.2253 404.2180
2458 405.2256 404.2183
2459 405.2263 404.2190
2460 405.2265 404.2192
2461 405.2268 404.2195
2462 405.2270 404.2197
2463 405.2271 404.2198
2464 405.2274 404.2201
2465 405.2479 404.2406
2466 405.2480 404.2407
2467 407.1313 406.1240
2468 407.1314 406.1241
2469 407.1494 406.1421
2470 407.1700 406.1627
2471 407.1700 406.1627
2472 407.1700 406.1627
2473 407.1700 406.1627
2474 407.1701 406.1628
2475 407.1701 406.1628
2476 407.1701 406.1628
2477 407.1703 406.1630
2478 407.1703 406.1630
2479 407.1703 406.1630
2480 407.1703 406.1630
2481 407.1705 406.1632
2482 407.1710 406.1637
2483 407.2060 406.1987
2484 407.2063 406.1990
2485 407.2064 406.1991
2486 407.2064 406.1991
2487 407.2064 406.1991
2488 407.2065 406.1992
2489 407.2065 406.1992
2490 407.2065 406.1992
2491 407.2066 406.1993
2492 407.2066 406.1993
2493 407.2067 406.1994
2494 407.2067 406.1994
2495 407.2071 406.1998
2496 407.2072 406.1999
2497 407.2076 406.2003
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