MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
698 325.1819 324.1746
699 325.1823 324.1750
700 325.1826 324.1753
701 325.2010 324.1937
702 325.2012 324.1939
703 325.2013 324.1940
704 325.2017 324.1944
705 325.2206 324.2133
706 326.1475 325.1402
707 326.1674 325.1601
708 326.1675 325.1602
709 326.1977 325.1904
710 327.0751 326.0678
711 327.0759 326.0686
712 327.0763 326.0690
713 327.0764 326.0691
714 327.0764 326.0691
715 327.1041 326.0968
716 327.1057 326.0984
717 327.1071 326.0998
718 327.1071 326.0998
719 327.1081 326.1008
720 327.1133 326.1060
721 327.1215 326.1142
722 327.1217 326.1144
723 327.1218 326.1145
724 327.1219 326.1146
725 327.1222 326.1149
726 327.1223 326.1150
727 327.1223 326.1150
728 327.1224 326.1151
729 327.1225 326.1152
730 327.1323 326.1250
731 327.1426 326.1353
732 327.1427 326.1354
733 327.1427 326.1354
734 327.1429 326.1356
735 327.1429 326.1356
736 327.1431 326.1358
737 327.1431 326.1358
738 327.1433 326.1360
739 327.1433 326.1360
740 327.1434 326.1361
741 327.1434 326.1361
742 327.1435 326.1362
743 327.1435 326.1362
744 327.1436 326.1363
745 327.1439 326.1366
746 327.1793 326.1720
747 327.1795 326.1722
748 327.1795 326.1722
749 327.1797 326.1724
750 327.1797 326.1724
751 327.1797 326.1724
752 327.1798 326.1725
753 327.1798 326.1725
754 327.1798 326.1725
755 327.1798 326.1725
756 327.1798 326.1725
757 327.1799 326.1726
758 327.1799 326.1726
759 327.1984 326.1911
760 327.1996 326.1923
761 327.1999 326.1926
762 327.2000 326.1927
763 327.2001 326.1928
764 327.2003 326.1930
765 327.2031 326.1958
766 327.2167 326.2094
767 328.0506 327.0433
768 328.2132 327.2059
769 328.2135 327.2062
770 328.2347 327.2274
771 329.0335 328.0262
772 329.0914 328.0841
773 329.0919 328.0846
774 329.1307 328.1234
775 329.1374 328.1301
776 329.1377 328.1304
777 329.1379 328.1306
778 329.1568 328.1495
779 329.1575 328.1502
780 329.1588 328.1515
781 329.1588 328.1515
782 329.1588 328.1515
783 329.1588 328.1515
784 329.1589 328.1516
785 329.1589 328.1516
786 329.1589 328.1516
787 329.1590 328.1517
788 329.1595 328.1522
789 329.1595 328.1522
790 329.1740 328.1667
791 329.1764 328.1691
792 330.2290 329.2217
793 330.2291 329.2218
794 331.0788 330.0715
795 331.0853 330.0780
796 331.1167 330.1094
797 331.1168 330.1095
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