MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
598 323.0836 322.0763
599 323.1108 322.1035
600 323.1112 322.1039
601 323.1113 322.1040
602 323.1113 322.1040
603 323.1115 322.1042
604 323.1117 322.1044
605 323.1117 322.1044
606 323.1118 322.1045
607 323.1118 322.1045
608 323.1119 322.1046
609 323.1129 322.1056
610 323.1137 322.1064
611 323.1277 322.1204
612 323.1476 322.1403
613 323.1478 322.1405
614 323.1479 322.1406
615 323.1480 322.1407
616 323.1481 322.1408
617 323.1481 322.1408
618 323.1481 322.1408
619 323.1482 322.1409
620 323.1482 322.1409
621 323.1482 322.1409
622 323.1482 322.1409
623 323.1483 322.1410
624 323.1483 322.1410
625 323.1483 322.1410
626 323.1483 322.1410
627 323.1483 322.1410
628 323.1483 322.1410
629 323.1484 322.1411
630 323.1484 322.1411
631 323.1484 322.1411
632 323.1497 322.1424
633 323.1657 322.1584
634 323.1657 322.1584
635 323.1658 322.1585
636 323.1664 322.1591
637 323.1664 322.1591
638 323.1680 322.1607
639 323.1847 322.1774
640 323.1848 322.1775
641 323.1848 322.1775
642 323.1848 322.1775
643 323.1848 322.1775
644 323.1848 322.1775
645 323.1849 322.1776
646 323.1849 322.1776
647 323.1850 322.1777
648 323.1850 322.1777
649 323.1850 322.1777
650 323.1851 322.1778
651 323.1851 322.1778
652 323.1851 322.1778
653 323.1851 322.1778
654 323.1852 322.1779
655 323.1852 322.1779
656 323.1855 322.1782
657 323.1863 322.1790
658 323.1864 322.1791
659 323.2043 322.1970
660 325.0606 324.0533
661 325.0869 324.0796
662 325.0903 324.0830
663 325.0908 324.0835
664 325.0999 324.0926
665 325.1068 324.0995
666 325.1072 324.0999
667 325.1265 324.1192
668 325.1269 324.1196
669 325.1272 324.1199
670 325.1273 324.1200
671 325.1273 324.1200
672 325.1273 324.1200
673 325.1274 324.1201
674 325.1274 324.1201
675 325.1274 324.1201
676 325.1275 324.1202
677 325.1275 324.1202
678 325.1276 324.1203
679 325.1278 324.1205
680 325.1280 324.1207
681 325.1281 324.1208
682 325.1338 324.1265
683 325.1632 324.1559
684 325.1637 324.1564
685 325.1637 324.1564
686 325.1639 324.1566
687 325.1639 324.1566
688 325.1639 324.1566
689 325.1639 324.1566
690 325.1640 324.1567
691 325.1640 324.1567
692 325.1640 324.1567
693 325.1641 324.1568
694 325.1641 324.1568
695 325.1642 324.1569
696 325.1642 324.1569
697 325.1644 324.1571
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