MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4266)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
498 319.1537 318.1464
499 319.1537 318.1464
500 319.1539 318.1466
501 319.1549 318.1476
502 319.1669 318.1596
503 319.1681 318.1608
504 319.1895 318.1822
505 319.1896 318.1823
506 319.1896 318.1823
507 319.1897 318.1824
508 319.1897 318.1824
509 319.1897 318.1824
510 319.1898 318.1825
511 319.1898 318.1825
512 319.1898 318.1825
513 319.1898 318.1825
514 319.1899 318.1826
515 319.1900 318.1827
516 319.1902 318.1829
517 319.2260 318.2187
518 319.2261 318.2188
519 319.2263 318.2190
520 319.2263 318.2190
521 319.2263 318.2190
522 320.2600 319.2527
523 320.2964 319.2891
524 320.2967 319.2894
525 321.0638 320.0565
526 321.0669 320.0596
527 321.0937 320.0864
528 321.0940 320.0867
529 321.0952 320.0879
530 321.0957 320.0884
531 321.0959 320.0886
532 321.0961 320.0888
533 321.0973 320.0900
534 321.1122 320.1049
535 321.1305 320.1232
536 321.1314 320.1241
537 321.1315 320.1242
538 321.1318 320.1245
539 321.1320 320.1247
540 321.1321 320.1248
541 321.1321 320.1248
542 321.1322 320.1249
543 321.1323 320.1250
544 321.1323 320.1250
545 321.1323 320.1250
546 321.1324 320.1251
547 321.1325 320.1252
548 321.1325 320.1252
549 321.1325 320.1252
550 321.1326 320.1253
551 321.1326 320.1253
552 321.1326 320.1253
553 321.1326 320.1253
554 321.1326 320.1253
555 321.1327 320.1254
556 321.1543 320.1470
557 321.1684 320.1611
558 321.1688 320.1615
559 321.1689 320.1616
560 321.1689 320.1616
561 321.1689 320.1616
562 321.1689 320.1616
563 321.1690 320.1617
564 321.1690 320.1617
565 321.1690 320.1617
566 321.1691 320.1618
567 321.1691 320.1618
568 321.1691 320.1618
569 321.1691 320.1618
570 321.1691 320.1618
571 321.1692 320.1619
572 321.1692 320.1619
573 321.1692 320.1619
574 321.1692 320.1619
575 321.1693 320.1620
576 321.1694 320.1621
577 321.1694 320.1621
578 321.1695 320.1622
579 321.1696 320.1623
580 321.2053 320.1980
581 321.2054 320.1981
582 321.2055 320.1982
583 321.2055 320.1982
584 321.2056 320.1983
585 321.2056 320.1983
586 321.2056 320.1983
587 321.2057 320.1984
588 321.2418 320.2345
589 321.2420 320.2347
590 321.2420 320.2347
591 321.2420 320.2347
592 321.2603 320.2530
593 322.1884 321.1811
594 322.1887 321.1814
595 322.9831 321.9758
596 323.0488 322.0415
597 323.0728 322.0655
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