| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 3 |
| MS data file | : | PRT1231_JBEI_3.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 1 May 2025 at 20:39:58 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,459 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 316.6824 | 631.3502 | 631.3475 | 4.27 | 0 | 22 | 0.1 | 1Score > 25 indicates identity |
LCIAR | |
| 373.2288 | 744.4430 | 744.4494 | -8.49 | 1 | 22 | 0.088 | 1Score > 24 indicates identity |
RVLQTK + Deamidated (NQ) | |
| 665.3540 | 664.3467 | 664.3432 | 5.35 | 0 | 22 | 0.058 | 1Score > 22 indicates identity |
EIFEK | |
| 465.2646 | 928.5146 | 928.5090 | 6.10 | 1 | 21 | 0.093 | 1Score > 24 indicates identity |
IRENLER | |
| 580.8016 | 1159.5886 | 1159.6084 | -17.0 | 1 | 20 | 0.12 | 1Score > 24 indicates identityScore > 23 indicates homology |
IAAKQQQATAK + 3 Deamidated (NQ) | |
| 330.6786 | 659.3426 | 659.3391 | 5.39 | 0 | 20 | 0.2 | 1Score > 25 indicates identity |
WVEAR | |
| 390.2281 | 778.4416 | 778.4378 | 5.00 | 0 | 20 | 0.066 | 1Score > 20 indicates identity |
FGLPAFK | |
| 402.6891 | 803.3636 | 803.3524 | 14.1 | 0 | 19 | 0.086 | 1Score > 22 indicates identityScore > 21 indicates homology |
EYAFMK + Oxidation (M) | |
| 430.2503 | 858.4860 | 858.4811 | 5.81 | 0 | 19 | 0.16 | 1Score > 24 indicates identity |
IAIVNSDK | |
| 502.3266 | 501.3193 | 501.3275 | -16.2 | 1 | 19 | 0.25 | 1Score > 26 indicates identity |
GGKLK | |
| 451.2434 | 900.4722 | 900.4777 | -6.05 | 1 | 19 | 0.15 | 1Score > 23 indicates identity |
AAVQARQR + 2 Deamidated (NQ) | |
| 745.4493 | 744.4420 | 744.4381 | 5.24 | 0 | 18 | 0.21 | 1Score > 24 indicates identity |
LAATLEK | |
| 393.7292 | 785.4438 | 785.4395 | 5.48 | 0 | 18 | 0.19 | 1Score > 23 indicates identity |
LGKPTDR | |
| 346.7122 | 691.4098 | 691.4017 | 11.8 | 1 | 17 | 0.071 | 1Score > 20 indicates identityScore > 18 indicates homology |
QRLFK + Deamidated (NQ) | |
| 494.7589 | 987.5032 | 987.4985 | 4.80 | 0 | 17 | 0.27 | 1Score > 24 indicates identity |
LQALDGSQR + Deamidated (NQ) | |
| 607.8171 | 1213.6196 | 1213.6237 | -3.35 | 1 | 17 | 0.065 | 1Score > 23 indicates identityScore > 18 indicates homology |
NCLQKIAPDR | |
| 401.2346 | 800.4546 | 800.4504 | 5.30 | 1 | 17 | 0.29 | 1Score > 25 indicates identityScore > 24 indicates homology |
LAKANQR + Deamidated (NQ) | |
| 444.2534 | 886.4922 | 886.4984 | -6.99 | 1 | 17 | 0.14 | 1Score > 25 indicates identityScore > 21 indicates homology |
AVDALRSR | |
| 387.8862 | 1160.6368 | 1160.6315 | 4.50 | 1 | 17 | 0.22 | 1Score > 23 indicates identity |
RHTWGHLVR | |
| 586.3953 | 585.3880 | 585.3962 | -14.0 | 1 | 17 | 0.19 | 1Score > 22 indicates identity |
KVIAR | |
| 426.2188 | 850.4230 | 850.4297 | -7.81 | 1 | 17 | 0.22 | 1Score > 24 indicates identityScore > 22 indicates homology |
YADQAKR | |
| 330.2099 | 658.4052 | 658.4126 | -11.2 | 1 | 16 | 0.079 | 1Score > 20 indicates identityScore > 18 indicates homology |
GKVTVR | |
| 478.2542 | 954.4938 | 954.4883 | 5.85 | 1 | 16 | 0.16 | 1Score > 21 indicates identityScore > 21 indicates homology |
KLASDHER | |
| 428.7688 | 855.5230 | 855.5290 | -6.97 | 1 | 16 | 0.14 | 1Score > 20 indicates identity |
LARIDLR | |
| 499.2743 | 996.5340 | 996.5141 | 20.0 | 1 | 16 | 0.18 | 1Score > 21 indicates identity |
VSFRDAFR | |
| 352.2047 | 702.3948 | 702.3912 | 5.21 | 0 | 16 | 0.4 | 1Score > 26 indicates identityScore > 24 indicates homology |
SLADAVK | |
| 300.6725 | 599.3304 | 599.3391 | -14.4 | 0 | 16 | 0.15 | 1Score > 20 indicates identity |
ASLGPR | |
| 522.8096 | 1043.6046 | 1043.5863 | 17.6 | 1 | 16 | 0.22 | 1Score > 22 indicates identity |
TTPLEEVKK | |
| 500.7595 | 999.5044 | 999.4920 | 12.5 | 1 | 15 | 0.16 | 1Score > 22 indicates identityScore > 20 indicates homology |
MVNEHRAK + Oxidation (M) | |
| 672.4076 | 671.4003 | 671.3966 | 5.54 | 0 | 15 | 0.45 | 1Score > 24 indicates identity |
LSSIPR | |
| 314.7249 | 627.4352 | 627.4432 | -12.6 | 1 | 15 | 0.069 | 1Score > 16 indicates identity |
LVLKR | |
| 364.7263 | 727.4380 | 727.4341 | 5.47 | 0 | 15 | 0.12 | 1Score > 19 indicates identity |
IGVAVNR | |
| 416.2454 | 1245.7144 | 1245.7041 | 8.24 | 1 | 15 | 0.16 | 1Score > 20 indicates identity |
RSATTIVSQVGK | |
| 565.3365 | 1128.6584 | 1128.6543 | 3.71 | 0 | 15 | 0.099 | 1Score > 20 indicates identityScore > 18 indicates homology |
WINTIQLIK + Deamidated (NQ) | |
| 433.2451 | 864.4756 | 864.4705 | 5.93 | 0 | 15 | 0.18 | 1Score > 20 indicates identity |
LVTTFQR + Deamidated (NQ) | |
| 437.7502 | 873.4858 | 873.4807 | 5.86 | 0 | 15 | 0.43 | 1Score > 25 indicates identityScore > 24 indicates homology |
ISADIVEK | |
| 580.8021 | 1159.5896 | 1159.6084 | -16.2 | 1 | 14 | 0.4 | 1Score > 24 indicates identityScore > 23 indicates homology |
IAAKQQQATAK + 3 Deamidated (NQ) | |
| 372.2441 | 742.4736 | 742.4701 | 4.77 | 1 | 14 | 0.26 | 1Score > 21 indicates identity |
KLGQIGK | |
| 351.7066 | 701.3986 | 701.3959 | 3.87 | 0 | 14 | 0.52 | 1Score > 24 indicates identity |
VEIVNK + Deamidated (NQ) | |
| 435.7769 | 869.5392 | 869.5334 | 6.71 | 0 | 14 | 0.12 | 1Score > 18 indicates identity |
VALNILAR + Deamidated (NQ) | |
| 372.5428 | 1114.6066 | 1114.5982 | 7.51 | 0 | 14 | 0.48 | 1Score > 23 indicates identity |
AVNQTIAQIR + 2 Deamidated (NQ) | |
| 609.8232 | 1217.6318 | 1217.6186 | 10.9 | 1 | 14 | 0.58 | 1Score > 24 indicates identity |
QVMRSAVAQAR + 2 Deamidated (NQ) | |
| 348.8749 | 1043.6029 | 1043.5863 | 15.9 | 1 | 14 | 0.091 | 1Score > 22 indicates identityScore > 16 indicates homology |
TTPLEEVKK | |
| 558.3124 | 1114.6102 | 1114.6168 | -5.91 | 1 | 14 | 0.27 | 1Score > 23 indicates identityScore > 21 indicates homology |
KPGSMNLPKK + Oxidation (M) | |
| 443.7556 | 885.4966 | 885.4920 | 5.30 | 1 | 14 | 0.5 | 1Score > 23 indicates identityScore > 23 indicates homology |
KGGPLASQK + Deamidated (NQ) | |
| 337.2174 | 672.4202 | 672.4170 | 4.80 | 0 | 13 | 0.36 | 1Score > 22 indicates identity |
VSIIGGK | |
| 578.3257 | 1154.6368 | 1154.6295 | 6.36 | 1 | 13 | 0.35 | 1Score > 21 indicates identity |
KTEKPQQPAK + Deamidated (NQ) | |
| 494.7783 | 987.5420 | 987.5349 | 7.23 | 0 | 13 | 0.072 | 1Score > 24 indicates identityScore > 14 indicates homology |
SIDTVVAQR | |
| 439.7426 | 877.4706 | 877.4658 | 5.57 | 0 | 13 | 0.2 | 1Score > 23 indicates identityScore > 19 indicates homology |
LNTLFDR | |
| 458.2501 | 914.4856 | 914.4821 | 3.86 | 0 | 13 | 0.34 | 1Score > 23 indicates identityScore > 21 indicates homology |
EQLAADLR | |
| 490.5919 | 1468.7539 | 1468.7423 | 7.90 | 1 | 13 | 0.085 | 1Score > 23 indicates identityScore > 15 indicates homology |
GGAPWIQNDGKVAR + Deamidated (NQ) | |
| 404.2421 | 806.4696 | 806.4650 | 5.71 | 0 | 13 | 0.26 | 1Score > 20 indicates identity |
VVLGYTR | |
| 544.7916 | 1087.5686 | 1087.5556 | 12.0 | 1 | 13 | 0.39 | 1Score > 25 indicates identityScore > 21 indicates homology |
MNPSRSLQR | |
| 314.6755 | 627.3364 | 627.3340 | 3.88 | 0 | 13 | 0.25 | 1Score > 19 indicates identity |
LGPEGR | |
| 569.3688 | 568.3615 | 568.3697 | -14.3 | 0 | 13 | 0.088 | 1Score > 15 indicates identity |
IPIAR | |
| 508.2827 | 1014.5508 | 1014.5458 | 4.98 | 1 | 13 | 0.84 | 1Score > 24 indicates identityScore > 24 indicates homology |
QLQDVQRK + Deamidated (NQ) | |
| 437.2578 | 872.5010 | 872.4902 | 12.4 | 1 | 12 | 0.6 | 1Score > 24 indicates identityScore > 23 indicates homology |
RCILSPK | |
| 361.8439 | 1082.5099 | 1082.4992 | 9.85 | 1 | 12 | 0.09 | 1Score > 21 indicates identityScore > 15 indicates homology |
SYGSEKANAR + Deamidated (NQ) | |
| 314.7065 | 627.3984 | 627.3956 | 4.61 | 0 | 12 | 0.21 | 1Score > 18 indicates identity |
TVIPAK | |
| 473.7480 | 945.4814 | 945.4953 | -14.7 | 0 | 12 | 0.58 | 1Score > 24 indicates identityScore > 23 indicates homology |
QMALVQEK | |
| 572.2887 | 1142.5628 | 1142.5832 | -17.8 | 1 | 12 | 0.47 | 1Score > 22 indicates identity |
ADWRTQLPR + Deamidated (NQ) | |
| 458.7610 | 915.5074 | 915.4913 | 17.6 | 0 | 12 | 0.43 | 1Score > 24 indicates identityScore > 21 indicates homology |
TTPLEEVK | |
| 509.7647 | 1017.5148 | 1017.4978 | 16.7 | 0 | 12 | 0.48 | 1Score > 25 indicates identityScore > 22 indicates homology |
TIANDNEIK + Deamidated (NQ) | |
| 461.7505 | 921.4864 | 921.4920 | -5.98 | 0 | 12 | 0.55 | 1Score > 23 indicates identityScore > 22 indicates homology |
SATANAFLK | |
| 564.8074 | 1127.6002 | 1127.6186 | -16.3 | 0 | 12 | 0.11 | 1Score > 22 indicates identityScore > 15 indicates homology |
IDILINNVGR + 2 Deamidated (NQ) | |
| 628.4057 | 627.3984 | 627.3956 | 4.57 | 0 | 12 | 0.24 | 1Score > 18 indicates identity |
TVIPAK | |
| 468.7627 | 935.5108 | 935.5188 | -8.53 | 1 | 12 | 0.097 | 1Score > 21 indicates identityScore > 14 indicates homology |
REYLSLR | |
| 559.3249 | 1116.6352 | 1116.6291 | 5.47 | 1 | 12 | 0.51 | 1Score > 21 indicates identity |
VPGRDGLLYK | |
| 600.3422 | 1198.6698 | 1198.6669 | 2.42 | 1 | 12 | 0.54 | 1Score > 22 indicates identity |
ENVIVNRLNK + Deamidated (NQ) | |
| 453.7209 | 905.4272 | 905.4276 | -0.44 | 0 | 12 | 0.74 | 1Score > 23 indicates identityScore > 23 indicates homology |
IQEEMTR | |
| 303.8364 | 908.4874 | 908.4716 | 17.4 | 1 | 11 | 0.53 | 1Score > 21 indicates identity |
NSSAFVKR + Deamidated (NQ) | |
| 461.2585 | 920.5024 | 920.4967 | 6.21 | 0 | 11 | 0.31 | 1Score > 22 indicates identityScore > 19 indicates homology |
AFGDTIVAK | |
| 689.8918 | 1377.7690 | 1377.7517 | 12.6 | 1 | 11 | 0.16 | 1Score > 20 indicates identityScore > 16 indicates homology |
FQAFGRPVKNSK | |
| 743.4453 | 742.4380 | 742.4337 | 5.78 | 0 | 11 | 0.87 | 1Score > 24 indicates identityScore > 23 indicates homology |
TGLSIPR | |
| 749.0688 | 2244.1846 | 2244.1559 | 12.8 | 0 | 11 | 0.44 | 1Score > 21 indicates identityScore > 20 indicates homology |
MGLGVLEDTALAQVPGTSDILK + Deamidated (NQ); Oxidation (M) | |
| 364.2286 | 726.4426 | 726.4388 | 5.28 | 0 | 11 | 0.27 | 1Score > 18 indicates identity |
IQLTPR | |
| 414.9204 | 1241.7394 | 1241.7166 | 18.4 | 0 | 11 | 0.2 | 1Score > 16 indicates identity |
IPAMGATSLIIR | |
| 402.2190 | 802.4234 | 802.4337 | -12.8 | 0 | 11 | 0.4 | 1Score > 25 indicates identityScore > 19 indicates homology |
WDTLIR | |
| 489.2393 | 976.4640 | 976.4760 | -12.3 | 0 | 11 | 0.14 | 1Score > 24 indicates identityScore > 15 indicates homology |
ILGGQSCSR | |
| 515.3322 | 514.3249 | 514.3227 | 4.28 | 0 | 11 | 0.67 | 1Score > 26 indicates identityScore > 22 indicates homology |
LGAVR | |
| 450.7815 | 899.5484 | 899.5440 | 4.95 | 0 | 11 | 0.39 | 1Score > 20 indicates identityScore > 19 indicates homology |
SLLGLLER | |
| 449.7920 | 897.5694 | 897.5647 | 5.26 | 0 | 11 | 0.17 | 1Score > 15 indicates identity |
QLIVALNK | |
| 632.2994 | 1262.5842 | 1262.5601 | 19.1 | 0 | 11 | 0.52 | 1Score > 23 indicates identityScore > 20 indicates homology |
ADMVASGDPSWK | |
| 343.8400 | 1028.4982 | 1028.4814 | 16.3 | 0 | 11 | 0.72 | 1Score > 22 indicates identity |
INNASFSFK + 2 Deamidated (NQ) | |
| 318.1807 | 634.3468 | 634.3438 | 4.74 | 0 | 10 | 0.43 | 1Score > 22 indicates identityScore > 19 indicates homology |
YLSPR | |
| 324.1753 | 646.3360 | 646.3286 | 11.5 | 1 | 10 | 0.71 | 1Score > 26 indicates identityScore > 21 indicates homology |
KEGGQK + Deamidated (NQ) | |
| 420.7527 | 839.4908 | 839.4865 | 5.20 | 1 | 10 | 0.31 | 1Score > 18 indicates identity |
KTIEPPR | |
| 742.4136 | 741.4063 | 741.4021 | 5.70 | 0 | 10 | 0.43 | 1Score > 19 indicates identity |
DPVNGLK | |
| 451.7706 | 901.5266 | 901.5120 | 16.2 | 0 | 10 | 0.33 | 1Score > 21 indicates identityScore > 18 indicates homology |
SLDLDIVK | |
| 319.1780 | 954.5122 | 954.5148 | -2.71 | 1 | 10 | 0.32 | 1Score > 21 indicates identityScore > 18 indicates homology |
NLYRHPR | |
| 767.4015 | 1532.7884 | 1532.8021 | -8.89 | 1 | 10 | 0.21 | 1Score > 24 indicates identityScore > 16 indicates homology |
FISQPGIMREDLK | |
| 311.6831 | 621.3516 | 621.3486 | 4.90 | 0 | 10 | 0.42 | 1Score > 20 indicates identityScore > 19 indicates homology |
ASAVFK | |
| 503.2847 | 502.2774 | 502.2751 | 4.62 | 0 | 10 | 0.95 | 1Score > 29 indicates identityScore > 22 indicates homology |
DAIGK | |
| 374.2309 | 746.4472 | 746.4439 | 4.49 | 0 | 10 | 0.46 | 1Score > 19 indicates identity |
ALQLFR | |
| 440.2469 | 878.4792 | 878.4749 | 4.95 | 0 | 10 | 0.59 | 1Score > 20 indicates identity |
LIYLDNK + Deamidated (NQ) | |
| 554.3364 | 553.3291 | 553.3336 | -8.12 | 0 | 10 | 0.11 | 1Score > 13 indicates identity |
KPGPR | |
| 436.7661 | 871.5176 | 871.5127 | 5.66 | 0 | 10 | 0.99 | 1Score > 23 indicates identityScore > 22 indicates homology |
SSIPITVR | |
| 409.7229 | 817.4312 | 817.4294 | 2.30 | 0 | 10 | 0.43 | 1Score > 25 indicates identityScore > 18 indicates homology |
LGSQSGIR + Deamidated (NQ) | |
| 395.2291 | 788.4436 | 788.4280 | 19.9 | 0 | 9 | 1 | 1Score > 23 indicates identityScore > 22 indicates homology |
EVEALTK | |
| 478.2636 | 954.5126 | 954.5208 | -8.55 | 1 | 9 | 0.75 | 1Score > 21 indicates identity |
ASLMKVYK + Oxidation (M) |
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