MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4266)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3369 316.6824 631.3502 631.3475 4.27 0 22 0.1 +1Score > 25 indicates identity LCIAR
3458 373.2288 744.4430 744.4494 -8.49 1 22 0.088 +1Score > 24 indicates identity RVLQTK + Deamidated (NQ)
3401 665.3540 664.3467 664.3432 5.35 0 22 0.058 +1Score > 22 indicates identity EIFEK
3612 465.2646 928.5146 928.5090 6.10 1 21 0.093 +1Score > 24 indicates identity IRENLER
3801 580.8016 1159.5886 1159.6084 -17.0 1 20 0.12 +1Score > 24 indicates identity
Score > 23 indicates homology
IAAKQQQATAK + 3 Deamidated (NQ)
3398 330.6786 659.3426 659.3391 5.39 0 20 0.2 +1Score > 25 indicates identity WVEAR
3477 390.2281 778.4416 778.4378 5.00 0 20 0.066 +1Score > 20 indicates identity FGLPAFK
3500 402.6891 803.3636 803.3524 14.1 0 19 0.086 +1Score > 22 indicates identity
Score > 21 indicates homology
EYAFMK + Oxidation (M)
3545 430.2503 858.4860 858.4811 5.81 0 19 0.16 +1Score > 24 indicates identity IAIVNSDK
3291 502.3266 501.3193 501.3275 -16.2 1 19 0.25 +1Score > 26 indicates identity GGKLK
3584 451.2434 900.4722 900.4777 -6.05 1 19 0.15 +1Score > 23 indicates identity AAVQARQR + 2 Deamidated (NQ)
3457 745.4493 744.4420 744.4381 5.24 0 18 0.21 +1Score > 24 indicates identity LAATLEK
3481 393.7292 785.4438 785.4395 5.48 0 18 0.19 +1Score > 23 indicates identity LGKPTDR
3418 346.7122 691.4098 691.4017 11.8 1 17 0.071 +1Score > 20 indicates identity
Score > 18 indicates homology
QRLFK + Deamidated (NQ)
3660 494.7589 987.5032 987.4985 4.80 0 17 0.27 +1Score > 24 indicates identity LQALDGSQR + Deamidated (NQ)
3834 607.8171 1213.6196 1213.6237 -3.35 1 17 0.065 +1Score > 23 indicates identity
Score > 18 indicates homology
NCLQKIAPDR
3495 401.2346 800.4546 800.4504 5.30 1 17 0.29 +1Score > 25 indicates identity
Score > 24 indicates homology
LAKANQR + Deamidated (NQ)
3570 444.2534 886.4922 886.4984 -6.99 1 17 0.14 +1Score > 25 indicates identity
Score > 21 indicates homology
AVDALRSR
3804 387.8862 1160.6368 1160.6315 4.50 1 17 0.22 +1Score > 23 indicates identity RHTWGHLVR
3335 586.3953 585.3880 585.3962 -14.0 1 17 0.19 +1Score > 22 indicates identity KVIAR
3540 426.2188 850.4230 850.4297 -7.81 1 17 0.22 +1Score > 24 indicates identity
Score > 22 indicates homology
YADQAKR
3397 330.2099 658.4052 658.4126 -11.2 1 16 0.079 +1Score > 20 indicates identity
Score > 18 indicates homology
GKVTVR
3634 478.2542 954.4938 954.4883 5.85 1 16 0.16 +1Score > 21 indicates identity
Score > 21 indicates homology
KLASDHER
3542 428.7688 855.5230 855.5290 -6.97 1 16 0.14 +1Score > 20 indicates identity LARIDLR
3667 499.2743 996.5340 996.5141 20.0 1 16 0.18 +1Score > 21 indicates identity VSFRDAFR
3426 352.2047 702.3948 702.3912 5.21 0 16 0.4 +1Score > 26 indicates identity
Score > 24 indicates homology
SLADAVK
3340 300.6725 599.3304 599.3391 -14.4 0 16 0.15 +1Score > 20 indicates identity ASLGPR
3701 522.8096 1043.6046 1043.5863 17.6 1 16 0.22 +1Score > 22 indicates identity TTPLEEVKK
3668 500.7595 999.5044 999.4920 12.5 1 15 0.16 +1Score > 22 indicates identity
Score > 20 indicates homology
MVNEHRAK + Oxidation (M)
3405 672.4076 671.4003 671.3966 5.54 0 15 0.45 +1Score > 24 indicates identity LSSIPR
3364 314.7249 627.4352 627.4432 -12.6 1 15 0.069 +1Score > 16 indicates identity LVLKR
3445 364.7263 727.4380 727.4341 5.47 0 15 0.12 +1Score > 19 indicates identity IGVAVNR
3856 416.2454 1245.7144 1245.7041 8.24 1 15 0.16 +1Score > 20 indicates identity RSATTIVSQVGK
3779 565.3365 1128.6584 1128.6543 3.71 0 15 0.099 +1Score > 20 indicates identity
Score > 18 indicates homology
WINTIQLIK + Deamidated (NQ)
3550 433.2451 864.4756 864.4705 5.93 0 15 0.18 +1Score > 20 indicates identity LVTTFQR + Deamidated (NQ)
3560 437.7502 873.4858 873.4807 5.86 0 15 0.43 +1Score > 25 indicates identity
Score > 24 indicates homology
ISADIVEK
3802 580.8021 1159.5896 1159.6084 -16.2 1 14 0.4 +1Score > 24 indicates identity
Score > 23 indicates homology
IAAKQQQATAK + 3 Deamidated (NQ)
3455 372.2441 742.4736 742.4701 4.77 1 14 0.26 +1Score > 21 indicates identity KLGQIGK
3425 351.7066 701.3986 701.3959 3.87 0 14 0.52 +1Score > 24 indicates identity VEIVNK + Deamidated (NQ)
3556 435.7769 869.5392 869.5334 6.71 0 14 0.12 +1Score > 18 indicates identity VALNILAR + Deamidated (NQ)
3761 372.5428 1114.6066 1114.5982 7.51 0 14 0.48 +1Score > 23 indicates identity AVNQTIAQIR + 2 Deamidated (NQ)
3839 609.8232 1217.6318 1217.6186 10.9 1 14 0.58 +1Score > 24 indicates identity QVMRSAVAQAR + 2 Deamidated (NQ)
3700 348.8749 1043.6029 1043.5863 15.9 1 14 0.091 +1Score > 22 indicates identity
Score > 16 indicates homology
TTPLEEVKK
3762 558.3124 1114.6102 1114.6168 -5.91 1 14 0.27 +1Score > 23 indicates identity
Score > 21 indicates homology
KPGSMNLPKK + Oxidation (M)
3568 443.7556 885.4966 885.4920 5.30 1 14 0.5 +1Score > 23 indicates identity
Score > 23 indicates homology
KGGPLASQK + Deamidated (NQ)
3407 337.2174 672.4202 672.4170 4.80 0 13 0.36 +1Score > 22 indicates identity VSIIGGK
3797 578.3257 1154.6368 1154.6295 6.36 1 13 0.35 +1Score > 21 indicates identity KTEKPQQPAK + Deamidated (NQ)
3662 494.7783 987.5420 987.5349 7.23 0 13 0.072 +1Score > 24 indicates identity
Score > 14 indicates homology
SIDTVVAQR
3562 439.7426 877.4706 877.4658 5.57 0 13 0.2 +1Score > 23 indicates identity
Score > 19 indicates homology
LNTLFDR
3596 458.2501 914.4856 914.4821 3.86 0 13 0.34 +1Score > 23 indicates identity
Score > 21 indicates homology
EQLAADLR
3999 490.5919 1468.7539 1468.7423 7.90 1 13 0.085 +1Score > 23 indicates identity
Score > 15 indicates homology
GGAPWIQNDGKVAR + Deamidated (NQ)
3504 404.2421 806.4696 806.4650 5.71 0 13 0.26 +1Score > 20 indicates identity VVLGYTR
3741 544.7916 1087.5686 1087.5556 12.0 1 13 0.39 +1Score > 25 indicates identity
Score > 21 indicates homology
MNPSRSLQR
3360 314.6755 627.3364 627.3340 3.88 0 13 0.25 +1Score > 19 indicates identity LGPEGR
3332 569.3688 568.3615 568.3697 -14.3 0 13 0.088 +1Score > 15 indicates identity IPIAR
3676 508.2827 1014.5508 1014.5458 4.98 1 13 0.84 +1Score > 24 indicates identity
Score > 24 indicates homology
QLQDVQRK + Deamidated (NQ)
3559 437.2578 872.5010 872.4902 12.4 1 12 0.6 +1Score > 24 indicates identity
Score > 23 indicates homology
RCILSPK
3730 361.8439 1082.5099 1082.4992 9.85 1 12 0.09 +1Score > 21 indicates identity
Score > 15 indicates homology
SYGSEKANAR + Deamidated (NQ)
3363 314.7065 627.3984 627.3956 4.61 0 12 0.21 +1Score > 18 indicates identity TVIPAK
3626 473.7480 945.4814 945.4953 -14.7 0 12 0.58 +1Score > 24 indicates identity
Score > 23 indicates homology
QMALVQEK
3787 572.2887 1142.5628 1142.5832 -17.8 1 12 0.47 +1Score > 22 indicates identity ADWRTQLPR + Deamidated (NQ)
3598 458.7610 915.5074 915.4913 17.6 0 12 0.43 +1Score > 24 indicates identity
Score > 21 indicates homology
TTPLEEVK
3680 509.7647 1017.5148 1017.4978 16.7 0 12 0.48 +1Score > 25 indicates identity
Score > 22 indicates homology
TIANDNEIK + Deamidated (NQ)
3607 461.7505 921.4864 921.4920 -5.98 0 12 0.55 +1Score > 23 indicates identity
Score > 22 indicates homology
SATANAFLK
3777 564.8074 1127.6002 1127.6186 -16.3 0 12 0.11 +1Score > 22 indicates identity
Score > 15 indicates homology
IDILINNVGR + 2 Deamidated (NQ)
3362 628.4057 627.3984 627.3956 4.57 0 12 0.24 +1Score > 18 indicates identity TVIPAK
3619 468.7627 935.5108 935.5188 -8.53 1 12 0.097 +1Score > 21 indicates identity
Score > 14 indicates homology
REYLSLR
3767 559.3249 1116.6352 1116.6291 5.47 1 12 0.51 +1Score > 21 indicates identity VPGRDGLLYK
3822 600.3422 1198.6698 1198.6669 2.42 1 12 0.54 +1Score > 22 indicates identity ENVIVNRLNK + Deamidated (NQ)
3589 453.7209 905.4272 905.4276 -0.44 0 12 0.74 +1Score > 23 indicates identity
Score > 23 indicates homology
IQEEMTR
3591 303.8364 908.4874 908.4716 17.4 1 11 0.53 +1Score > 21 indicates identity NSSAFVKR + Deamidated (NQ)
3606 461.2585 920.5024 920.4967 6.21 0 11 0.31 +1Score > 22 indicates identity
Score > 19 indicates homology
AFGDTIVAK
3954 689.8918 1377.7690 1377.7517 12.6 1 11 0.16 +1Score > 20 indicates identity
Score > 16 indicates homology
FQAFGRPVKNSK
3453 743.4453 742.4380 742.4337 5.78 0 11 0.87 +1Score > 24 indicates identity
Score > 23 indicates homology
TGLSIPR
4197 749.0688 2244.1846 2244.1559 12.8 0 11 0.44 +1Score > 21 indicates identity
Score > 20 indicates homology
MGLGVLEDTALAQVPGTSDILK + Deamidated (NQ); Oxidation (M)
3444 364.2286 726.4426 726.4388 5.28 0 11 0.27 +1Score > 18 indicates identity IQLTPR
3853 414.9204 1241.7394 1241.7166 18.4 0 11 0.2 +1Score > 16 indicates identity IPAMGATSLIIR
3497 402.2190 802.4234 802.4337 -12.8 0 11 0.4 +1Score > 25 indicates identity
Score > 19 indicates homology
WDTLIR
3654 489.2393 976.4640 976.4760 -12.3 0 11 0.14 +1Score > 24 indicates identity
Score > 15 indicates homology
ILGGQSCSR
3308 515.3322 514.3249 514.3227 4.28 0 11 0.67 +1Score > 26 indicates identity
Score > 22 indicates homology
LGAVR
3583 450.7815 899.5484 899.5440 4.95 0 11 0.39 +1Score > 20 indicates identity
Score > 19 indicates homology
SLLGLLER
3579 449.7920 897.5694 897.5647 5.26 0 11 0.17 +1Score > 15 indicates identity QLIVALNK
3869 632.2994 1262.5842 1262.5601 19.1 0 11 0.52 +1Score > 23 indicates identity
Score > 20 indicates homology
ADMVASGDPSWK
3688 343.8400 1028.4982 1028.4814 16.3 0 11 0.72 +1Score > 22 indicates identity INNASFSFK + 2 Deamidated (NQ)
3370 318.1807 634.3468 634.3438 4.74 0 10 0.43 +1Score > 22 indicates identity
Score > 19 indicates homology
YLSPR
3381 324.1753 646.3360 646.3286 11.5 1 10 0.71 +1Score > 26 indicates identity
Score > 21 indicates homology
KEGGQK + Deamidated (NQ)
3532 420.7527 839.4908 839.4865 5.20 1 10 0.31 +1Score > 18 indicates identity KTIEPPR
3452 742.4136 741.4063 741.4021 5.70 0 10 0.43 +1Score > 19 indicates identity DPVNGLK
3586 451.7706 901.5266 901.5120 16.2 0 10 0.33 +1Score > 21 indicates identity
Score > 18 indicates homology
SLDLDIVK
3635 319.1780 954.5122 954.5148 -2.71 1 10 0.32 +1Score > 21 indicates identity
Score > 18 indicates homology
NLYRHPR
4030 767.4015 1532.7884 1532.8021 -8.89 1 10 0.21 +1Score > 24 indicates identity
Score > 16 indicates homology
FISQPGIMREDLK
3355 311.6831 621.3516 621.3486 4.90 0 10 0.42 +1Score > 20 indicates identity
Score > 19 indicates homology
ASAVFK
3292 503.2847 502.2774 502.2751 4.62 0 10 0.95 +1Score > 29 indicates identity
Score > 22 indicates homology
DAIGK
3459 374.2309 746.4472 746.4439 4.49 0 10 0.46 -1Score > 19 indicates identity ALQLFR
3563 440.2469 878.4792 878.4749 4.95 0 10 0.59 +1Score > 20 indicates identity LIYLDNK + Deamidated (NQ)
3324 554.3364 553.3291 553.3336 -8.12 0 10 0.11 +1Score > 13 indicates identity KPGPR
3557 436.7661 871.5176 871.5127 5.66 0 10 0.99 +1Score > 23 indicates identity
Score > 22 indicates homology
SSIPITVR
3512 409.7229 817.4312 817.4294 2.30 0 10 0.43 +1Score > 25 indicates identity
Score > 18 indicates homology
LGSQSGIR + Deamidated (NQ)
3486 395.2291 788.4436 788.4280 19.9 0 9 1 +1Score > 23 indicates identity
Score > 22 indicates homology
EVEALTK
3636 478.2636 954.5126 954.5208 -8.55 1 9 0.75 +1Score > 21 indicates identity ASLMKVYK + Oxidation (M)
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