MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 36)

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+1

Accession Score Description
1 IGF1_BOVIN 1232 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 209 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 Q2U2R5_ASPOR 193 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

+4

Accession Score Description
1 Q2UK92_ASPOR 179 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 171 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 TRYP_PIG 160 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 Q2U034_ASPOR 149 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1

-8

Accession Score Description
1 Q2U287_ASPOR 82 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1
Score Mass Matches Sequences emPAI
8.1 Q2U287_ASPOR 82 59365 10 (5) 10 (5) 0.31
UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1

-10 peptide matches (10 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3345   302.1983 602.3820 602.3792 4.76 0 15 0.11 +1Score > 18 indicates identity U R.FLPVK.T
3385   327.1679 652.3212 652.3180 4.94 0 17 0.076 +1Score > 19 indicates identity K.SDLYR.L
3441   362.1889 722.3632 722.3599 4.62 0 14 0.31 +1Score > 22 indicates identity U K.VSDFQK.H
3509   408.7547 815.4948 815.4905 5.28 0 26 0.004 +1Score > 20 indicates identity
Score > 15 indicates homology		 U R.FGGVPVIK.L
3513   410.2479 818.4812 818.4763 6.10 0 8 0.83 +1Score > 20 indicates identity U K.HIPSIPR.I
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_3.raw

Score > 21 indicates identity
17.4 1 11 0.53 1 NSSAFVKR   + Deamidated (NQ)
-7.32 1 11 0.57 2 AIRHGAQR   + Deamidated (NQ)
3591   303.8364 908.4874 908.4828 5.05 0 10 0.74 -3Score > 21 indicates identity U R.QIEHLNR.T
5.05 0 5 2.1 4 HLQELNR  
5.05 1 5 2.3 5 YLSSGRAR  
5.03 0 1 5.4 6 GLALDHGAR  
17.4 1 1 6.2 7 FASSVNRK   + Deamidated (NQ)
17.4 1 1 6.4 8 ETTFKQR  
17.4 1 0 6.6 9 YSKDLQR  
3671   505.8189 1009.6232 1009.6172 6.03 0 40 0.00016 +1Score > 15 indicates identity U R.LLEIAQVPK.E
3709   524.7918 1047.5690 1047.5634 5.36 0 46 7.6e-005 +1Score > 23 indicates identity
Score > 17 indicates homology		 U K.TCSDLLLVK.S
3816   398.9229 1193.7469 1193.7383 7.16 1 14 0.039 +1Score > 13 indicates identity U R.IIKDSLLPAPK.S
3973   475.5726 1423.6960 1423.6878 5.75 0 16 0.049 +1Score > 24 indicates identity
Score > 16 indicates homology		 U R.LEHGQLVMDPNR.F + Oxidation (M)

+9

Accession Score Description
1 Q2U480_ASPOR 69 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+10

Accession Score Description
1 Q2UPG4_ASPOR 63 Delta-aminolevulinic acid dehydratase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001652 PE=3 SV=1
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