MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (36 proteins)


Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 IGF1_BOVIN 1232 42206 77 (55) 24 (20) 16.58 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal
2 FHP_YEAST 209 44846 13 (9) 10 (8) 0.90 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2
3 Q2U2R5_ASPOR 193 49765 14 (11) 14 (11) 1.03 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1
4 Q2UK92_ASPOR 179 63866 11 (6) 10 (6) 0.35 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1
5 Q2UHF6_ASPOR 171 54290 8 (7) 8 (7) 0.51 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1
6 TRYP_PIG 160 25078 7 (6) 4 (4) 1.12 Trypsin OS=Sus scrofa PE=1 SV=1
7 Q2U034_ASPOR 149 131681 5 (4) 5 (4) 0.10 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1
8 Q2U287_ASPOR 82 59365 10 (5) 10 (5) 0.31 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1
9 Q2U480_ASPOR 69 19581 6 (4) 6 (4) 0.89 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1
10 Q2UPG4_ASPOR 63 41087 3 (2) 3 (2) 0.17 Delta-aminolevulinic acid dehydratase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001652 PE=3 SV=1
11 Q2ULW0_ASPOR 53 84939 3 (2) 3 (2) 0.08 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000247 PE=4 SV=1
12 Q2UJR1_ASPOR 49 74750 8 (4) 8 (4) 0.19 Acetyl-coenzyme A synthetase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003001112 PE=3 SV=1
13 Q2U0N5_ASPOR 38 19103 1 (1) 1 (1) 0.18 GAT domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000365 PE=4 SV=1
14 Q2UU14_ASPOR 36 53719 1 (1) 1 (1) 0.06 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000505 PE=3 SV=1
15 Q2UPJ0_ASPOR 36 20065 4 (3) 4 (3) 0.59 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001622 PE=4 SV=1
16 Q2UR52_ASPOR 34 64720 2 (1) 1 (1) 0.05 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000967 PE=4 SV=1
17 Q2UK62_ASPOR 32 75289 2 (1) 2 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000939 PE=4 SV=1
18 Q2UKB4_ASPOR 32 91359 2 (1) 1 (1) 0.04 Urease OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000879 PE=3 SV=1
19 Q2U0S3_ASPOR 32 0 1 (1) 1 (1) 0.03 description
20 Q2U1V7_ASPOR 30 35502 1 (1) 1 (1) 0.09 ANK_REP_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090138000080 PE=4 SV=1
21 Q2UV06_ASPOR 30 81360 2 (1) 2 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090009000100 PE=4 SV=1
22 Q2TZC1_ASPOR 28 56447 2 (2) 2 (2) 0.12 Serine hydroxymethyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000915 PE=3 SV=1
23 Q2TVX9_ASPOR 28 84507 1 (1) 1 (1) 0.04 Actin binding protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=Aoabp1 PE=4 SV=1
24 Q2U9Y9_ASPOR 27 0 1 (1) 1 (1) 0.04 description
25 Q2UCH1_ASPOR 26 31180 1 (1) 1 (1) 0.11 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090012000591 PE=4 SV=1
26 Q2U385_ASPOR 26 32548 2 (1) 2 (1) 0.10 RelA_SpoT domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000141 PE=4 SV=1
27 PEPA_ASPOR 25 0 1 (1) 1 (1) 0.07 description
28 Q2UFH8_ASPOR 23 137000 2 (1) 2 (1) 0.02 SF3b1 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090026000203 PE=4 SV=1
29 Q2U8D0_ASPOR 23 26124 1 (1) 1 (1) 0.13 CIA30 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000476 PE=4 SV=1
30 Q2TWP0_ASPOR 23 55369 1 (1) 1 (1) 0.06 ATP synthase subunit beta OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090010000482 PE=3 SV=1
31 Q2UQ81_ASPOR 23 82237 1 (1) 1 (1) 0.04 AP complex subunit beta OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001357 PE=3 SV=1
32 Q2UHE7_ASPOR 22 73756 1 (1) 1 (1) 0.04 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000477 PE=4 SV=1
33 Q2UHA9_ASPOR 21 39464 1 (1) 1 (1) 0.08 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000518 PE=4 SV=1
34 Q2U9F0_ASPOR 18 165829 2 (1) 2 (1) 0.02 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000055 PE=4 SV=1
35 Q2U724_ASPOR 15 62067 1 (1) 1 (1) 0.05 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090120000001 PE=4 SV=1
36 Q2U984_ASPOR 15 31060 1 (1) 1 (1) 0.11 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090701000133 PE=3 SV=1

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