MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 36)

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+1

Accession Score Description
1 IGF1_BOVIN 1232 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 209 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

+3

Accession Score Description
1 Q2U2R5_ASPOR 193 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

-4

Accession Score Description
1 Q2UK92_ASPOR 179 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1
Score Mass Matches Sequences emPAI
4.1 Q2UK92_ASPOR 179 63866 11 (6) 10 (6) 0.35
AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

-11 peptide matches (11 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3483   394.7187 787.4228 787.4188 5.13 0 3 1 +2Score > 26 indicates identity
Score > 15 indicates homology		 U R.QLSSPTR.E
3511   409.6865 817.3584 817.3541 5.33 0 1 3.3 +1Score > 19 indicates identity U K.WMNDPR.V
3616   466.2686 930.5226 930.5175 5.58 0 44 0.00057 +1Score > 24 indicates identity U R.VPYLPSQK.T
3726   538.2800 1074.5454 1074.5379 6.99 0 41 0.00026 +1Score > 25 indicates identity
Score > 18 indicates homology		 U R.TETVMLEPR.V
3745   546.2766 1090.5386 1090.5329 5.31 0 13 0.71 +1Score > 24 indicates identity U R.TETVMLEPR.V + Oxidation (M)
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_3.raw

Score > 24 indicates identity
3918   667.3486 1332.6826 1332.6786 3.03 0 44 0.00062 -1Score > 24 indicates identity U R.LGQLIGGAGNYDR.G
No other peptide matches in query
3969   471.9238 1412.7496 1412.7412 5.93 0 38 0.00034 +1Score > 23 indicates identity
Score > 16 indicates homology		 U R.GIHLLVGEQEYR.G
3977   715.3811 1428.7476 1428.7401 5.25 0 50 5.5e-005 +1Score > 24 indicates identity
Score > 19 indicates homology		 U R.YIPSVGQYLSFR.V
4180   515.2890 2057.1269 2057.0959 15.1 0 3 2.3 +1Score > 19 indicates identity U R.HPIRPKPPHQGDVFYIR.Y + Deamidated (NQ)
4183   695.3487 2083.0243 2083.0123 5.76 0 66 3.2e-006 +1Score > 23 indicates identity U R.VSAAWGEAGPISHQEEFLR.N
4225   623.8312 2491.2957 2491.2608 14.0 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology		 U R.LFVFSLSPLNPGQNVPGSSHHTR.Q + Deamidated (NQ)

+5

Accession Score Description
1 Q2UHF6_ASPOR 171 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 TRYP_PIG 160 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 Q2U034_ASPOR 149 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1

+8

Accession Score Description
1 Q2U287_ASPOR 82 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1

+9

Accession Score Description
1 Q2U480_ASPOR 69 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+10

Accession Score Description
1 Q2UPG4_ASPOR 63 Delta-aminolevulinic acid dehydratase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001652 PE=3 SV=1
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