MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 36)

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+1

Accession Score Description
1 IGF1_BOVIN 1232 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 FHP_YEAST 209 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

-3

Accession Score Description
1 Q2U2R5_ASPOR 193 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1
Score Mass Matches Sequences emPAI
3.1 Q2U2R5_ASPOR 193 49765 14 (11) 14 (11) 1.03
WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

-14 peptide matches (14 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3417   345.1967 688.3788 688.3756 4.77 0 15 0.66 +1Score > 26 indicates identity U K.GTVADVK.C
3421   350.2129 698.4112 698.4075 5.34 0 41 0.00042 +1Score > 20 indicates identity U R.GLAVSPR.H
3430   353.6729 705.3312 705.3268 6.27 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology		 U R.VWDMR.T
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_3.raw

Score > 20 indicates identity

Score > 19 indicates homology
3515   410.7452 819.4758 819.4715 5.31 0 24 0.015 -1Score > 20 indicates identity
Score > 19 indicates homology		 U R.NLAIHPR.E
5.31 0 5 1.3 2 INHLPAR  
3537   423.7344 845.4542 845.4494 5.70 0 30 0.016 +1Score > 25 indicates identity U R.LITGEADK.T
3558   437.2489 872.4832 872.4756 8.78 0 49 0.0002 +1Score > 25 indicates identity U R.LWDLAAGK.S
3622   472.2852 942.5558 942.5498 6.38 0 43 0.00052 +1Score > 23 indicates identity U R.LDLLVTGGR.D
3760   557.8251 1113.6356 1113.6295 5.56 0 50 9.4e-005 +1Score > 23 indicates identity U K.IWNLATGALR.L
3774   375.2169 1122.6289 1122.6298 -0.85 0 12 0.28 +1Score > 18 indicates identity U R.VISGHLGWVR.S
3814   396.9019 1187.6839 1187.6761 6.52 1 29 0.0055 +1Score > 19 indicates identity U R.LITGEADKTIK.V
3819   399.9049 1196.6929 1196.6877 4.30 0 30 0.0049 +1Score > 19 indicates identity U R.LTLTGHISTVR.G
3921   673.3397 1344.6648 1344.6561 6.47 0 57 5.5e-006 +1Score > 24 indicates identity
Score > 17 indicates homology		 U R.EFTFASASTGSIK.Q
3931   676.8037 1351.5928 1351.5867 4.56 0 28 0.013 +1Score > 21 indicates identity U R.HPYLFSCGEDK.M
4155   960.9542 1919.8938 1919.8649 15.1 0 25 0.043 +1Score > 24 indicates identity U R.SLAVEPNNEWFASGAGDR.T + Deamidated (NQ)

+4

Accession Score Description
1 Q2UK92_ASPOR 179 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 171 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 TRYP_PIG 160 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 Q2U034_ASPOR 149 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1

+8

Accession Score Description
1 Q2U287_ASPOR 82 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1

+9

Accession Score Description
1 Q2U480_ASPOR 69 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+10

Accession Score Description
1 Q2UPG4_ASPOR 63 Delta-aminolevulinic acid dehydratase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001652 PE=3 SV=1
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