MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 3
MS data file : PRT1231_JBEI_3.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:58 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,459

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 36)

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+1

Accession Score Description
1 IGF1_BOVIN 1232 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

-2

Accession Score Description
1 FHP_YEAST 209 Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2
Score Mass Matches Sequences emPAI
2.1 FHP_YEAST 209 44846 13 (9) 10 (8) 0.90
Flavohemoprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YHB1 PE=1 SV=2

-13 peptide matches (13 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3785   568.7740 1135.5334 1135.5258 6.76 0 45 0.00042 +1Score > 24 indicates identity U R.QENQYDALR.H
3795   577.2722 1152.5298 1152.5233 5.64 0 17 0.028 +1Score > 23 indicates identity
Score > 14 indicates homology		 U R.HYSLCSASTK.N
3830   602.3250 1202.6354 1202.6295 4.93 0 62 1e-005 +1Score > 24 indicates identity U K.LSAPAGDFAINK.E
4012   500.2605 1497.7597 1497.7497 6.64 0 47 7.8e-005 +1Score > 23 indicates identity
Score > 19 indicates homology		 U K.NIDDLSVLMDHVK.Q
4022   505.5914 1513.7524 1513.7446 5.11 0 13 0.69 +1Score > 24 indicates identity U K.NIDDLSVLMDHVK.Q + Oxidation (M)
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_3.raw

Score > 17 indicates identity
4073   532.6450 1594.9132 1594.9042 5.60 0 14 0.099 -1Score > 17 indicates identity U K.VGAQPNALATTVLAAAK.N
No other peptide matches in query
4074   798.4639 1594.9132 1594.9042 5.65 0 63 1.2e-006 +1Score > 17 indicates identity U K.VGAQPNALATTVLAAAK.N
4075   535.2785 1602.8137 1602.8042 5.92 0 15 0.055 +1Score > 24 indicates identity
Score > 15 indicates homology		 U R.ENFPAGLVSEYLHK.D
4082   806.4610 1610.9074 1610.8992 5.13 0 56 1.2e-005 +1Score > 19 indicates identity U K.ATVPVLEQQGTVITR.T
4083   537.9772 1610.9098 1610.8992 6.57 0 22 0.025 +1Score > 19 indicates identity U K.ATVPVLEQQGTVITR.T
4144   621.3182 1860.9328 1860.9040 15.5 0 31 0.0016 +1Score > 24 indicates identity
Score > 16 indicates homology		 U K.NMLTEHTELLNIFNR.T + Deamidated (NQ); Oxidation (M)
4151   632.3631 1894.0675 1894.0564 5.86 0 13 0.12 +1Score > 17 indicates identity U K.IIVHTDTEPLINAAFLK.E
4184   528.5560 2110.1949 2110.1826 5.82 0 15 0.059 +1Score > 15 indicates identity U R.ALQIKPEHYPIVGEYLLK.A

+3

Accession Score Description
1 Q2U2R5_ASPOR 193 WD_REPEATS_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000349 PE=4 SV=1

+4

Accession Score Description
1 Q2UK92_ASPOR 179 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+5

Accession Score Description
1 Q2UHF6_ASPOR 171 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+6

Accession Score Description
1 TRYP_PIG 160 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 Q2U034_ASPOR 149 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1

+8

Accession Score Description
1 Q2U287_ASPOR 82 UTP--glucose-1-phosphate uridylyltransferase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000558 PE=3 SV=1

+9

Accession Score Description
1 Q2U480_ASPOR 69 eIF-5a domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090020000450 PE=4 SV=1

+10

Accession Score Description
1 Q2UPG4_ASPOR 63 Delta-aminolevulinic acid dehydratase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005001652 PE=3 SV=1
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