MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
637 319.1898 318.1825
638 319.1900 318.1827
639 319.1900 318.1827
640 319.2258 318.2185
641 319.2259 318.2186
642 319.2260 318.2187
643 319.2262 318.2189
644 319.2263 318.2190
645 320.1348 319.1275
646 320.1350 319.1277
647 320.1351 319.1278
648 320.1716 319.1643
649 320.1716 319.1643
650 320.1718 319.1645
651 320.2083 319.2010
652 321.0568 320.0495
653 321.0651 320.0578
654 321.0783 320.0710
655 321.0933 320.0860
656 321.0933 320.0860
657 321.0941 320.0868
658 321.0943 320.0870
659 321.0947 320.0874
660 321.0948 320.0875
661 321.0950 320.0877
662 321.0951 320.0878
663 321.0952 320.0879
664 321.0955 320.0882
665 321.0959 320.0886
666 321.0969 320.0896
667 321.1299 320.1226
668 321.1308 320.1235
669 321.1308 320.1235
670 321.1313 320.1240
671 321.1314 320.1241
672 321.1316 320.1243
673 321.1317 320.1244
674 321.1322 320.1249
675 321.1322 320.1249
676 321.1322 320.1249
677 321.1322 320.1249
678 321.1323 320.1250
679 321.1323 320.1250
680 321.1323 320.1250
681 321.1323 320.1250
682 321.1323 320.1250
683 321.1324 320.1251
684 321.1324 320.1251
685 321.1324 320.1251
686 321.1324 320.1251
687 321.1324 320.1251
688 321.1325 320.1252
689 321.1339 320.1266
690 321.1560 320.1487
691 321.1687 320.1614
692 321.1688 320.1615
693 321.1688 320.1615
694 321.1688 320.1615
695 321.1688 320.1615
696 321.1689 320.1616
697 321.1689 320.1616
698 321.1689 320.1616
699 321.1689 320.1616
700 321.1689 320.1616
701 321.1690 320.1617
702 321.1690 320.1617
703 321.1690 320.1617
704 321.1690 320.1617
705 321.1691 320.1618
706 321.1691 320.1618
707 321.1692 320.1619
708 321.1692 320.1619
709 321.1692 320.1619
710 321.1693 320.1620
711 321.1694 320.1621
712 321.1695 320.1622
713 321.1695 320.1622
714 321.1696 320.1623
715 321.2050 320.1977
716 321.2050 320.1977
717 321.2052 320.1979
718 321.2053 320.1980
719 321.2057 320.1984
720 321.2416 320.2343
721 321.2418 320.2345
722 321.2423 320.2350
723 322.1504 321.1431
724 322.1508 321.1435
725 322.1876 321.1803
726 322.1884 321.1811
727 322.2238 321.2165
728 323.0177 322.0104
729 323.0485 322.0412
730 323.0488 322.0415
731 323.0715 322.0642
732 323.0751 322.0678
733 323.0764 322.0691
734 323.1105 322.1032
735 323.1105 322.1032
736 323.1112 322.1039
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