MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
537 317.1373 316.1300
538 317.1373 316.1300
539 317.1373 316.1300
540 317.1373 316.1300
541 317.1374 316.1301
542 317.1374 316.1301
543 317.1374 316.1301
544 317.1374 316.1301
545 317.1375 316.1302
546 317.1375 316.1302
547 317.1376 316.1303
548 317.1377 316.1304
549 317.1378 316.1305
550 317.1378 316.1305
551 317.1380 316.1307
552 317.1389 316.1316
553 317.1735 316.1662
554 317.1739 316.1666
555 317.1739 316.1666
556 317.1741 316.1668
557 317.1743 316.1670
558 317.1744 316.1671
559 317.1744 316.1671
560 317.1761 316.1688
561 317.1885 316.1812
562 318.0409 317.0336
563 318.1196 317.1123
564 318.1559 317.1486
565 318.1561 317.1488
566 318.1921 317.1848
567 318.1924 317.1851
568 318.1925 317.1852
569 318.1925 317.1852
570 318.1925 317.1852
571 318.1925 317.1852
572 318.1926 317.1853
573 318.1926 317.1853
574 318.1927 317.1854
575 319.0480 318.0407
576 319.0782 318.0709
577 319.0782 318.0709
578 319.0784 318.0711
579 319.0785 318.0712
580 319.0795 318.0722
581 319.0796 318.0723
582 319.0969 318.0896
583 319.1143 318.1070
584 319.1152 318.1079
585 319.1154 318.1081
586 319.1161 318.1088
587 319.1163 318.1090
588 319.1163 318.1090
589 319.1163 318.1090
590 319.1163 318.1090
591 319.1163 318.1090
592 319.1164 318.1091
593 319.1165 318.1092
594 319.1165 318.1092
595 319.1165 318.1092
596 319.1165 318.1092
597 319.1165 318.1092
598 319.1165 318.1092
599 319.1166 318.1093
600 319.1167 318.1094
601 319.1330 318.1257
602 319.1530 318.1457
603 319.1531 318.1458
604 319.1532 318.1459
605 319.1532 318.1459
606 319.1532 318.1459
607 319.1532 318.1459
608 319.1533 318.1460
609 319.1533 318.1460
610 319.1534 318.1461
611 319.1534 318.1461
612 319.1534 318.1461
613 319.1534 318.1461
614 319.1534 318.1461
615 319.1534 318.1461
616 319.1534 318.1461
617 319.1534 318.1461
618 319.1534 318.1461
619 319.1534 318.1461
620 319.1535 318.1462
621 319.1535 318.1462
622 319.1538 318.1465
623 319.1539 318.1466
624 319.1548 318.1475
625 319.1667 318.1594
626 319.1678 318.1605
627 319.1893 318.1820
628 319.1894 318.1821
629 319.1895 318.1822
630 319.1895 318.1822
631 319.1895 318.1822
632 319.1896 318.1823
633 319.1897 318.1824
634 319.1897 318.1824
635 319.1897 318.1824
636 319.1897 318.1824
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