MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
437 313.1638 312.1565
438 313.1639 312.1566
439 313.1642 312.1569
440 313.1643 312.1570
441 313.1643 312.1570
442 313.1643 312.1570
443 313.1649 312.1576
444 313.1649 312.1576
445 313.1655 312.1582
446 313.1827 312.1754
447 313.1829 312.1756
448 313.1833 312.1760
449 313.1837 312.1764
450 313.1837 312.1764
451 313.1841 312.1768
452 313.1844 312.1771
453 313.1844 312.1771
454 313.1844 312.1771
455 313.1846 312.1773
456 313.2206 312.2133
457 313.2387 312.2314
458 313.2388 312.2315
459 314.0461 313.0388
460 314.0462 313.0389
461 314.1975 313.1902
462 314.1976 313.1903
463 314.1978 313.1905
464 315.0395 314.0322
465 315.0677 314.0604
466 315.0747 314.0674
467 315.0760 314.0687
468 315.0853 314.0780
469 315.1031 314.0958
470 315.1054 314.0981
471 315.1071 314.0998
472 315.1134 314.1061
473 315.1143 314.1070
474 315.1209 314.1136
475 315.1211 314.1138
476 315.1215 314.1142
477 315.1216 314.1143
478 315.1217 314.1144
479 315.1217 314.1144
480 315.1218 314.1145
481 315.1220 314.1147
482 315.1221 314.1148
483 315.1221 314.1148
484 315.1424 314.1351
485 315.1425 314.1352
486 315.1425 314.1352
487 315.1427 314.1354
488 315.1441 314.1368
489 315.1446 314.1373
490 315.1449 314.1376
491 315.1450 314.1377
492 315.1451 314.1378
493 315.1452 314.1379
494 315.1453 314.1380
495 315.1454 314.1381
496 315.1458 314.1385
497 315.1464 314.1391
498 315.1577 314.1504
499 315.1578 314.1505
500 315.1580 314.1507
501 315.1580 314.1507
502 315.1581 314.1508
503 315.1582 314.1509
504 315.1583 314.1510
505 315.1586 314.1513
506 315.1589 314.1516
507 315.1596 314.1523
508 315.1600 314.1527
509 315.1814 314.1741
510 315.1815 314.1742
511 315.1815 314.1742
512 315.2008 314.1935
513 316.0247 315.0174
514 316.1396 315.1323
515 316.1763 315.1690
516 316.1765 315.1692
517 316.1767 315.1694
518 316.1769 315.1696
519 316.1769 315.1696
520 316.2134 315.2061
521 316.2136 315.2063
522 317.0569 316.0496
523 317.0814 316.0741
524 317.0992 316.0919
525 317.0994 316.0921
526 317.0997 316.0924
527 317.0998 316.0925
528 317.0999 316.0926
529 317.1000 316.0927
530 317.1000 316.0927
531 317.1002 316.0929
532 317.1003 316.0930
533 317.1003 316.0930
534 317.1005 316.0932
535 317.1010 316.0937
536 317.1372 316.1299
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