MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
337 309.2062 308.1989
338 309.2063 308.1990
339 309.2071 308.1998
340 309.2081 308.2008
341 310.0154 309.0081
342 311.0283 310.0210
343 311.0734 310.0661
344 311.0739 310.0666
345 311.0807 310.0734
346 311.0809 310.0736
347 311.0812 310.0739
348 311.0814 310.0741
349 311.0814 310.0741
350 311.0815 310.0742
351 311.0823 310.0750
352 311.0826 310.0753
353 311.0904 310.0831
354 311.0906 310.0833
355 311.0906 310.0833
356 311.1112 310.1039
357 311.1113 310.1040
358 311.1113 310.1040
359 311.1113 310.1040
360 311.1113 310.1040
361 311.1113 310.1040
362 311.1113 310.1040
363 311.1115 310.1042
364 311.1115 310.1042
365 311.1124 310.1051
366 311.1124 310.1051
367 311.1479 310.1406
368 311.1479 310.1406
369 311.1479 310.1406
370 311.1479 310.1406
371 311.1479 310.1406
372 311.1480 310.1407
373 311.1480 310.1407
374 311.1480 310.1407
375 311.1481 310.1408
376 311.1481 310.1408
377 311.1482 310.1409
378 311.1482 310.1409
379 311.1483 310.1410
380 311.1483 310.1410
381 311.1485 310.1412
382 311.1485 310.1412
383 311.1488 310.1415
384 311.1495 310.1422
385 311.1653 310.1580
386 311.1659 310.1586
387 311.1661 310.1588
388 311.1661 310.1588
389 311.1662 310.1589
390 311.1845 310.1772
391 311.1846 310.1773
392 311.1851 310.1778
393 311.2227 310.2154
394 311.2227 310.2154
395 311.2228 310.2155
396 311.2229 310.2156
397 311.2231 310.2158
398 311.2231 310.2158
399 312.0303 311.0230
400 312.0308 311.0235
401 312.1321 311.1248
402 312.1321 311.1248
403 312.1333 311.1260
404 312.1517 311.1444
405 312.1520 311.1447
406 312.1521 311.1448
407 312.1521 311.1448
408 312.2079 311.2006
409 313.0237 312.0164
410 313.0603 312.0530
411 313.0608 312.0535
412 313.0620 312.0547
413 313.0868 312.0795
414 313.0870 312.0797
415 313.0894 312.0821
416 313.0898 312.0825
417 313.0918 312.0845
418 313.0957 312.0884
419 313.0962 312.0889
420 313.0982 312.0909
421 313.0982 312.0909
422 313.1170 312.1097
423 313.1171 312.1098
424 313.1268 312.1195
425 313.1269 312.1196
426 313.1270 312.1197
427 313.1275 312.1202
428 313.1280 312.1207
429 313.1292 312.1219
430 313.1295 312.1222
431 313.1299 312.1226
432 313.1634 312.1561
433 313.1635 312.1562
434 313.1637 312.1564
435 313.1637 312.1564
436 313.1638 312.1565
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