MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
237 307.1532 306.1459
238 307.1533 306.1460
239 307.1533 306.1460
240 307.1533 306.1460
241 307.1534 306.1461
242 307.1534 306.1461
243 307.1537 306.1464
244 307.1538 306.1465
245 307.1897 306.1824
246 307.1899 306.1826
247 307.1899 306.1826
248 307.1899 306.1826
249 307.1900 306.1827
250 307.1900 306.1827
251 307.1900 306.1827
252 307.1901 306.1828
253 307.1901 306.1828
254 307.1901 306.1828
255 307.1901 306.1828
256 307.1902 306.1829
257 307.1902 306.1829
258 307.1902 306.1829
259 307.1903 306.1830
260 307.1903 306.1830
261 307.1905 306.1832
262 307.2081 306.2008
263 308.1200 307.1127
264 308.1351 307.1278
265 308.1505 307.1432
266 308.1565 307.1492
267 308.1575 307.1502
268 309.0668 308.0595
269 309.0929 308.0856
270 309.0937 308.0864
271 309.0948 308.0875
272 309.0949 308.0876
273 309.0951 308.0878
274 309.0954 308.0881
275 309.0955 308.0882
276 309.0958 308.0885
277 309.0963 308.0890
278 309.0970 308.0897
279 309.1116 308.1043
280 309.1306 308.1233
281 309.1314 308.1241
282 309.1318 308.1245
283 309.1319 308.1246
284 309.1319 308.1246
285 309.1319 308.1246
286 309.1320 308.1247
287 309.1320 308.1247
288 309.1320 308.1247
289 309.1320 308.1247
290 309.1321 308.1248
291 309.1322 308.1249
292 309.1323 308.1250
293 309.1323 308.1250
294 309.1324 308.1251
295 309.1324 308.1251
296 309.1324 308.1251
297 309.1324 308.1251
298 309.1324 308.1251
299 309.1325 308.1252
300 309.1325 308.1252
301 309.1325 308.1252
302 309.1325 308.1252
303 309.1328 308.1255
304 309.1330 308.1257
305 309.1384 308.1311
306 309.1386 308.1313
307 309.1395 308.1322
308 309.1499 308.1426
309 309.1505 308.1432
310 309.1687 308.1614
311 309.1688 308.1615
312 309.1688 308.1615
313 309.1688 308.1615
314 309.1689 308.1616
315 309.1689 308.1616
316 309.1689 308.1616
317 309.1689 308.1616
318 309.1689 308.1616
319 309.1689 308.1616
320 309.1689 308.1616
321 309.1689 308.1616
322 309.1689 308.1616
323 309.1690 308.1617
324 309.1690 308.1617
325 309.1690 308.1617
326 309.1690 308.1617
327 309.1690 308.1617
328 309.1690 308.1617
329 309.1690 308.1617
330 309.1690 308.1617
331 309.1691 308.1618
332 309.1694 308.1621
333 309.1762 308.1689
334 309.1886 308.1813
335 309.2055 308.1982
336 309.2062 308.1989
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