MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4073 357.2021 712.3896
4078 363.7021 725.3896
4079 364.2125 726.4104
4081 366.1987 730.3828
4082 368.1905 734.3664
4083 368.2072 734.3998
4084 368.2146 734.4146
4085 368.6697 735.3248
4087 743.4447 742.4374
4089 745.4490 744.4417
4091 374.1962 746.3778
4093 375.1803 748.3460
4095 377.2173 752.4200
4097 381.2128 760.4110
4099 386.7083 771.4020
4100 387.7411 773.4676
4101 388.2557 774.4968
4105 390.2282 778.4418
4106 392.7447 783.4748
4109 394.2318 786.4490
4112 394.7189 787.4232
4117 399.1670 796.3194
4118 399.2341 796.4536
4119 399.2368 796.4590
4121 401.2292 800.4438
4128 403.6805 805.3464
4132 405.2259 808.4372
4137 412.7548 823.4950
4138 413.1801 824.3456
4141 416.2393 830.4640
4144 418.7310 835.4474
4146 842.5128 841.5055
4152 425.7267 849.4388
4154 427.2097 852.4048
4155 428.2523 854.4900 854.4861 4.57 0     +0Score > 18 indicates identity EVEPLLR
4160 431.2487 860.4828
4161 432.2294 862.4442
4164 869.4770 868.4697
4165 435.7576 869.5006
4168 870.5450 869.5377
4169 437.2570 872.4994
4172 440.7341 879.4536
4175 446.7626 891.5106
4176 446.7628 891.5110
4180 451.2331 900.4516
4183 304.1561 909.4465
4184 455.7334 909.4522
4185 457.2421 912.4696
4186 457.7755 913.5364
4187 305.6646 913.9720
4188 458.2693 914.5240
4191 307.5128 919.5166
4193 308.1770 921.5092
4194 308.8323 923.4751
4195 309.5011 925.4815
4196 310.1722 927.4948
4197 310.5117 928.5133
4198 310.5142 928.5208
4199 465.7578 929.5010
4201 311.5028 931.4866
4202 312.1803 933.5191
4204 475.2736 948.5326
4206 320.1714 957.4924
4209 321.2417 960.7033
4211 322.1679 963.4819
4212 322.8518 965.5336
4213 485.7113 969.4080
4215 326.1719 975.4939
4219 494.2631 986.5116
4224 334.8672 1001.5798
4226 505.8185 1009.6224
4227 337.8362 1010.4868
4228 337.8470 1010.5192
4229 338.1812 1011.5218
4233 339.5396 1015.5970
4236 510.7801 1019.5456
4241 344.8676 1031.5810
4243 519.7670 1037.5194
4247 522.7963 1043.5780
4248 348.8746 1043.6020
4255 351.2367 1050.6883
4257 352.9140 1055.7202
4259 533.2662 1064.5178
4261 356.5348 1066.5826
4262 534.7850 1067.5554
4263 537.2311 1072.4476
4266 361.8433 1082.5081
4270 364.1636 1089.4690
4272 365.5263 1093.5571
4273 549.7505 1097.4864
4275 367.5346 1099.5820
4277 553.7984 1105.5822
4279 558.3088 1114.6030
4284 373.5088 1117.5046
4285 560.7939 1119.5732
4286 564.8069 1127.5992
4290 573.3066 1144.5986
4292 574.8642 1147.7138
4294 384.5539 1150.6399
4296 577.2991 1152.5836
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