MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3938 507.2230 506.2157
3939 507.2232 506.2159
3941 509.2384 508.2311
3942 509.2385 508.2312
3943 509.2385 508.2312
3944 509.2385 508.2312
3945 509.2386 508.2313
3946 509.2387 508.2314
3947 509.2388 508.2315
3948 509.2389 508.2316
3949 509.2390 508.2317
3950 509.2393 508.2320
3951 509.2394 508.2321
3952 511.2538 510.2465
3953 511.2545 510.2472
3954 511.2545 510.2472
3955 511.2549 510.2476
3956 511.2549 510.2476
3957 511.2549 510.2476
3958 513.2706 512.2633
3959 515.3324 514.3251
3960 517.1613 516.1540
3961 517.1892 516.1819
3962 520.3353 519.3280
3963 523.2543 522.2470
3964 525.2334 524.2261
3965 525.2703 524.2630
3966 525.2704 524.2631
3967 525.2904 524.2831
3969 527.1821 526.1748
3970 531.1154 530.1081
3971 533.2001 532.1928
3972 537.2341 536.2268
3973 537.2343 536.2270
3974 537.2711 536.2638
3975 545.3315 544.3242
3976 555.2439 554.2366
3978 567.2439 566.2366
3979 567.2447 566.2374
3980 567.2463 566.2390
3981 569.3179 568.3106
3982 569.3684 568.3611
3983 574.3208 573.3135
3985 581.2597 580.2524
3986 581.2598 580.2525
3988 595.2746 594.2673
3989 595.2752 594.2679
3991 301.1292 600.2438
3992 301.1423 600.2700
3993 302.1980 602.3814
3994 302.6696 603.3246
3995 305.1668 608.3190
3996 306.1576 610.3006
3998 613.2855 612.2782
4003 309.6595 617.3044
4004 311.6831 621.3516
4005 313.1293 624.2440
4006 313.1294 624.2442
4007 313.1302 624.2458
4008 625.2858 624.2785
4009 625.2885 624.2812
4010 314.1510 626.2874
4016 315.1119 628.2092
4017 315.1454 628.2762
4018 315.6837 629.3528
4020 317.0996 632.1846
4021 317.1944 632.3742
4025 643.2610 642.2537
4026 322.1490 642.2834
4027 323.1093 644.2040
4028 323.1110 644.2074
4029 323.2020 644.3894
4032 327.1435 652.2724
4033 327.1436 652.2726
4036 329.1243 656.2340
4037 329.1253 656.2360
4038 329.1257 656.2368
4039 329.1270 656.2394
4040 329.6887 657.3628
4041 658.3909 657.3836
4043 330.1914 658.3682
4045 330.2099 658.4052
4048 335.1498 668.2850
4050 336.1630 670.3114
4051 672.4071 671.3998
4052 336.7133 671.4120
4053 337.1245 672.2344
4055 341.1219 680.2292
4056 341.6991 681.3836
4057 683.3286 682.3213
4058 343.1396 684.2646
4059 343.1404 684.2662
4060 343.1408 684.2670
4061 688.3910 687.3837
4062 345.1777 688.3408
4064 350.7231 699.4316
4065 701.3599 700.3526
4070 352.7224 703.4302
4071 357.1323 712.2500
4072 357.1545 712.2944
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