MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
137 305.1368 304.1295
138 305.1370 304.1297
139 305.1372 304.1299
140 305.1372 304.1299
141 305.1373 304.1300
142 305.1373 304.1300
143 305.1374 304.1301
144 305.1374 304.1301
145 305.1374 304.1301
146 305.1374 304.1301
147 305.1374 304.1301
148 305.1374 304.1301
149 305.1374 304.1301
150 305.1374 304.1301
151 305.1374 304.1301
152 305.1374 304.1301
153 305.1374 304.1301
154 305.1375 304.1302
155 305.1375 304.1302
156 305.1375 304.1302
157 305.1375 304.1302
158 305.1375 304.1302
159 305.1375 304.1302
160 305.1375 304.1302
161 305.1585 304.1512
162 305.1736 304.1663
163 305.1738 304.1665
164 305.1738 304.1665
165 305.1738 304.1665
166 305.1739 304.1666
167 305.1739 304.1666
168 305.1739 304.1666
169 305.1739 304.1666
170 305.1739 304.1666
171 305.1740 304.1667
172 305.1740 304.1667
173 305.1740 304.1667
174 305.1740 304.1667
175 305.1742 304.1669
176 305.1742 304.1669
177 305.1743 304.1670
178 305.1743 304.1670
179 305.1744 304.1671
180 305.1748 304.1675
181 305.1750 304.1677
182 305.1844 304.1771
183 305.2105 304.2032
184 305.9590 304.9517
185 306.1206 305.1133
186 306.1559 305.1486
187 306.1559 305.1486
188 306.1560 305.1487
189 306.1561 305.1488
190 306.1562 305.1489
191 306.1563 305.1490
192 306.1923 305.1850
193 306.1923 305.1850
194 307.0094 306.0021
195 307.0208 306.0135
196 307.0774 306.0701
197 307.0780 306.0707
198 307.0783 306.0710
199 307.0786 306.0713
200 307.0788 306.0715
201 307.0807 306.0734
202 307.0809 306.0736
203 307.0960 306.0887
204 307.1156 306.1083
205 307.1159 306.1086
206 307.1162 306.1089
207 307.1162 306.1089
208 307.1163 306.1090
209 307.1163 306.1090
210 307.1165 306.1092
211 307.1165 306.1092
212 307.1165 306.1092
213 307.1165 306.1092
214 307.1165 306.1092
215 307.1166 306.1093
216 307.1167 306.1094
217 307.1167 306.1094
218 307.1167 306.1094
219 307.1167 306.1094
220 307.1168 306.1095
221 307.1169 306.1096
222 307.1518 306.1445
223 307.1526 306.1453
224 307.1529 306.1456
225 307.1530 306.1457
226 307.1530 306.1457
227 307.1530 306.1457
228 307.1530 306.1457
229 307.1530 306.1457
230 307.1531 306.1458
231 307.1531 306.1458
232 307.1531 306.1458
233 307.1532 306.1459
234 307.1532 306.1459
235 307.1532 306.1459
236 307.1532 306.1459
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