MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4391)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3637 455.2277 454.2204
3638 455.2278 454.2205
3639 455.2278 454.2205
3640 455.2279 454.2206
3641 455.2281 454.2208
3642 455.2697 454.2624
3643 455.2701 454.2628
3644 455.2702 454.2629
3645 455.2702 454.2629
3646 455.2702 454.2629
3647 455.2702 454.2629
3648 455.2708 454.2635
3649 457.1372 456.1299
3650 457.1404 456.1331
3651 457.1418 456.1345
3652 457.2065 456.1992
3653 457.2249 456.2176
3654 457.2250 456.2177
3655 457.2252 456.2179
3656 457.2252 456.2179
3657 457.2252 456.2179
3658 457.2252 456.2179
3659 457.2253 456.2180
3660 457.2253 456.2180
3661 457.2256 456.2183
3662 457.2257 456.2184
3663 457.2257 456.2184
3664 457.2430 456.2357
3665 457.2435 456.2362
3666 457.2439 456.2366
3667 457.2440 456.2367
3668 459.1198 458.1125
3669 459.1604 458.1531
3670 459.2198 458.2125
3671 459.2243 458.2170
3672 461.1428 460.1355
3673 461.2164 460.2091
3674 461.2173 460.2100
3675 461.2174 460.2101
3676 461.2563 460.2490
3677 461.2563 460.2490
3678 461.2565 460.2492
3679 461.2571 460.2498
3680 463.1388 462.1315
3681 463.1572 462.1499
3682 463.1958 462.1885
3683 463.1960 462.1887
3684 463.1961 462.1888
3685 463.1962 462.1889
3686 463.1967 462.1894
3687 463.1968 462.1895
3688 463.2320 462.2247
3689 463.2324 462.2251
3690 463.2325 462.2252
3691 463.2327 462.2254
3692 463.2327 462.2254
3693 463.2329 462.2256
3694 463.2329 462.2256
3695 463.2330 462.2257
3696 463.2332 462.2259
3697 463.2340 462.2267
3698 463.2342 462.2269
3699 463.2904 462.2831
3700 465.1726 464.1653
3701 465.1753 464.1680
3702 465.1754 464.1681
3703 465.2115 464.2042
3704 465.2115 464.2042
3705 465.2115 464.2042
3706 465.2119 464.2046
3707 465.2119 464.2046
3708 465.2120 464.2047
3709 465.2121 464.2048
3710 465.2121 464.2048
3711 465.2121 464.2048
3712 465.2122 464.2049
3713 465.2123 464.2050
3714 465.2125 464.2052
3715 465.2126 464.2053
3716 465.2129 464.2056
3717 465.2485 464.2412
3718 465.2486 464.2413
3719 465.2487 464.2414
3720 465.2488 464.2415
3721 465.2490 464.2417
3722 465.2495 464.2422
3723 465.2497 464.2424
3724 467.0919 466.0846
3725 467.1908 466.1835
3726 467.1913 466.1840
3727 467.1916 466.1843
3728 467.1918 466.1845
3729 467.1923 466.1850
3730 467.1923 466.1850
3731 467.2274 466.2201
3732 467.2274 466.2201
3733 467.2275 466.2202
3734 467.2275 466.2202
3735 467.2275 466.2202
3736 467.2277 466.2204
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