MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3237 423.2378 422.2305
3238 423.2384 422.2311
3239 423.2393 422.2320
3240 425.1510 424.1437
3241 425.1510 424.1437
3242 425.1796 424.1723
3243 425.1802 424.1729
3244 425.1802 424.1729
3245 425.1803 424.1730
3246 425.1803 424.1730
3247 425.1803 424.1730
3248 425.1804 424.1731
3249 425.1805 424.1732
3250 425.1805 424.1732
3251 425.1805 424.1732
3252 425.1806 424.1733
3253 425.1806 424.1733
3254 425.1807 424.1734
3255 425.1807 424.1734
3256 425.1807 424.1734
3257 425.2160 424.2087
3258 425.2162 424.2089
3259 425.2164 424.2091
3260 425.2167 424.2094
3261 425.2167 424.2094
3262 425.2170 424.2097
3263 425.2170 424.2097
3264 425.2172 424.2099
3265 425.2173 424.2100
3266 425.2174 424.2101
3267 425.2175 424.2102
3268 425.2181 424.2108
3269 425.2182 424.2109
3270 425.2189 424.2116
3271 425.2190 424.2117
3272 425.2345 424.2272
3273 425.2350 424.2277
3274 425.2351 424.2278
3275 425.2353 424.2280
3276 425.2354 424.2281
3277 425.2354 424.2281
3278 425.2356 424.2283
3279 425.2888 424.2815
3280 425.2888 424.2815
3281 425.2894 424.2821
3282 425.2894 424.2821
3283 425.2895 424.2822
3284 426.2376 425.2303
3285 426.2384 425.2311
3286 427.0935 426.0862
3287 427.1316 426.1243
3288 427.1325 426.1252
3289 427.1598 426.1525
3290 427.1670 426.1597
3291 427.1753 426.1680
3292 427.1957 426.1884
3293 427.1958 426.1885
3294 427.1961 426.1888
3295 427.1961 426.1888
3296 427.1962 426.1889
3297 427.1963 426.1890
3298 427.1964 426.1891
3299 427.1964 426.1891
3300 427.1967 426.1894
3301 427.1969 426.1896
3302 427.1983 426.1910
3303 427.1984 426.1911
3304 427.2327 426.2254
3305 427.2328 426.2255
3306 427.2328 426.2255
3307 427.2329 426.2256
3308 427.2329 426.2256
3309 427.2329 426.2256
3310 427.2329 426.2256
3311 427.2329 426.2256
3312 427.2330 426.2257
3313 427.2330 426.2257
3314 427.2332 426.2259
3315 427.2508 426.2435
3316 427.2509 426.2436
3317 428.2537 427.2464
3318 428.2538 427.2465
3319 428.2539 427.2466
3320 428.2545 427.2472
3321 428.2887 427.2814
3322 429.1082 428.1009
3323 429.1083 428.1010
3324 429.1466 428.1393
3325 429.1472 428.1399
3326 429.1536 428.1463
3327 429.1539 428.1466
3328 429.1826 428.1753
3329 430.0942 429.0869
3330 430.2427 429.2354
3331 430.3003 429.2930
3332 430.3012 429.2939
3333 431.0875 430.0802
3334 432.2822 431.2749
3335 432.2824 431.2751
3336 433.1480 432.1407
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