MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2837 401.2176 400.2103
2838 401.2186 400.2113
2839 401.2188 400.2115
2840 402.2479 401.2406
2841 402.2479 401.2406
2842 403.0680 402.0607
2843 403.0935 402.0862
2844 403.1366 402.1293
2845 403.1728 402.1655
2846 403.1746 402.1673
2847 403.1747 402.1674
2848 403.1747 402.1674
2849 403.1748 402.1675
2850 403.1748 402.1675
2851 403.1748 402.1675
2852 403.1749 402.1676
2853 403.1749 402.1676
2854 403.1750 402.1677
2855 403.1752 402.1679
2856 403.1753 402.1680
2857 403.1758 402.1685
2858 403.1760 402.1687
2859 403.1768 402.1695
2860 403.2113 402.2040
2861 403.2117 402.2044
2862 403.2119 402.2046
2863 403.2120 402.2047
2864 403.2122 402.2049
2865 403.2331 402.2258
2866 404.2663 403.2590
2867 405.1151 404.1078
2868 405.1155 404.1082
2869 405.1537 404.1464
2870 405.1540 404.1467
2871 405.1540 404.1467
2872 405.1541 404.1468
2873 405.1542 404.1469
2874 405.1542 404.1469
2875 405.1542 404.1469
2876 405.1543 404.1470
2877 405.1543 404.1470
2878 405.1544 404.1471
2879 405.1901 404.1828
2880 405.1902 404.1829
2881 405.1904 404.1831
2882 405.1904 404.1831
2883 405.1905 404.1832
2884 405.1906 404.1833
2885 405.1906 404.1833
2886 405.1906 404.1833
2887 405.1906 404.1833
2888 405.1907 404.1834
2889 405.1907 404.1834
2890 405.1908 404.1835
2891 405.1908 404.1835
2892 405.1908 404.1835
2893 405.1909 404.1836
2894 405.1909 404.1836
2895 405.1912 404.1839
2896 405.1916 404.1843
2897 405.2089 404.2016
2898 405.2259 404.2186
2899 405.2260 404.2187
2900 405.2260 404.2187
2901 405.2260 404.2187
2902 405.2271 404.2198
2903 405.2277 404.2204
2904 405.2477 404.2404
2905 405.2479 404.2406
2906 407.0946 406.0873
2907 407.1309 406.1236
2908 407.1311 406.1238
2909 407.1316 406.1243
2910 407.1693 406.1620
2911 407.1697 406.1624
2912 407.1697 406.1624
2913 407.1698 406.1625
2914 407.1699 406.1626
2915 407.1699 406.1626
2916 407.1699 406.1626
2917 407.1700 406.1627
2918 407.1700 406.1627
2919 407.1700 406.1627
2920 407.1700 406.1627
2921 407.1700 406.1627
2922 407.1701 406.1628
2923 407.1701 406.1628
2924 407.1702 406.1629
2925 407.1704 406.1631
2926 407.2060 406.1987
2927 407.2061 406.1988
2928 407.2062 406.1989
2929 407.2062 406.1989
2930 407.2062 406.1989
2931 407.2062 406.1989
2932 407.2063 406.1990
2933 407.2063 406.1990
2934 407.2063 406.1990
2935 407.2064 406.1991
2936 407.2065 406.1992
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