MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4391)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2737 397.1851 396.1778
2738 397.1852 396.1779
2739 397.1852 396.1779
2740 397.1853 396.1780
2741 397.1854 396.1781
2742 397.1854 396.1781
2743 397.1854 396.1781
2744 397.1854 396.1781
2745 397.1855 396.1782
2746 397.1855 396.1782
2747 397.1855 396.1782
2748 397.1856 396.1783
2749 397.1856 396.1783
2750 397.1856 396.1783
2751 397.1856 396.1783
2752 397.1856 396.1783
2753 397.1857 396.1784
2754 397.1857 396.1784
2755 397.1859 396.1786
2756 397.1861 396.1788
2757 397.1862 396.1789
2758 397.1863 396.1790
2759 397.1870 396.1797
2760 397.1889 396.1816
2761 397.2041 396.1968
2762 397.2044 396.1971
2763 397.2044 396.1971
2764 397.2045 396.1972
2765 397.2046 396.1973
2766 397.2046 396.1973
2767 397.2048 396.1975
2768 397.2059 396.1986
2769 397.2063 396.1990
2770 397.2213 396.2140
2771 397.2215 396.2142
2772 397.2216 396.2143
2773 397.2218 396.2145
2774 397.2230 396.2157
2775 397.2242 396.2169
2776 397.2247 396.2174
2777 399.0990 398.0917
2778 399.0999 398.0926
2779 399.1238 398.1165
2780 399.1630 398.1557
2781 399.1635 398.1562
2782 399.1651 398.1578
2783 399.1657 398.1584
2784 399.1657 398.1584
2785 399.1658 398.1585
2786 399.1659 398.1586
2787 399.1664 398.1591
2788 399.1813 398.1740
2789 399.1827 398.1754
2790 399.2002 398.1929
2791 399.2008 398.1935
2792 399.2011 398.1938
2793 399.2011 398.1938
2794 399.2012 398.1939
2795 399.2013 398.1940
2796 399.2013 398.1940
2797 399.2014 398.1941
2798 399.2014 398.1941
2799 399.2014 398.1941
2800 399.2014 398.1941
2801 399.2014 398.1941
2802 399.2015 398.1942
2803 399.2034 398.1961
2804 399.2202 398.2129
2805 399.2211 398.2138
2806 399.2211 398.2138
2807 399.2212 398.2139
2808 399.2215 398.2142
2809 399.2215 398.2142
2810 400.0824 399.0751
2811 401.0795 400.0722
2812 401.1135 400.1062
2813 401.1140 400.1067
2814 401.1165 400.1092
2815 401.1214 400.1141
2816 401.1409 400.1336
2817 401.1577 400.1504
2818 401.1590 400.1517
2819 401.1591 400.1518
2820 401.1592 400.1519
2821 401.1593 400.1520
2822 401.1593 400.1520
2823 401.1594 400.1521
2824 401.1596 400.1523
2825 401.1597 400.1524
2826 401.1599 400.1526
2827 401.1604 400.1531
2828 401.2168 400.2095
2829 401.2168 400.2095
2830 401.2169 400.2096
2831 401.2169 400.2096
2832 401.2169 400.2096
2833 401.2169 400.2096
2834 401.2170 400.2097
2835 401.2171 400.2098
2836 401.2173 400.2100
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