MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
37 301.1422 300.1349
38 301.1422 300.1349
39 301.1422 300.1349
40 301.1422 300.1349
41 301.1423 300.1350
42 301.1423 300.1350
43 301.1423 300.1350
44 301.1423 300.1350
45 301.1423 300.1350
46 301.1423 300.1350
47 301.1423 300.1350
48 301.1424 300.1351
49 301.1424 300.1351
50 301.1424 300.1351
51 301.1425 300.1352
52 301.1425 300.1352
53 301.1426 300.1353
54 301.1426 300.1353
55 301.1426 300.1353
56 301.1426 300.1353
57 301.1427 300.1354
58 301.1429 300.1356
59 301.1429 300.1356
60 301.1435 300.1362
61 301.1437 300.1364
62 301.1633 300.1560
63 301.1645 300.1572
64 301.2207 300.2134
65 302.0098 301.0025
66 302.1611 301.1538
67 302.1611 301.1538
68 302.1974 301.1901
69 302.1975 301.1902
70 302.1975 301.1902
71 302.1976 301.1903
72 302.1977 301.1904
73 302.2340 301.2267
74 302.8699 301.8626
75 303.0410 302.0337
76 303.0514 302.0441
77 303.0677 302.0604
78 303.0741 302.0668
79 303.0751 302.0678
80 303.0756 302.0683
81 303.0835 302.0762
82 303.0837 302.0764
83 303.0840 302.0767
84 303.0842 302.0769
85 303.0844 302.0771
86 303.0845 302.0772
87 303.0849 302.0776
88 303.0850 302.0777
89 303.0851 302.0778
90 303.1028 302.0955
91 303.1213 302.1140
92 303.1213 302.1140
93 303.1214 302.1141
94 303.1214 302.1141
95 303.1214 302.1141
96 303.1215 302.1142
97 303.1216 302.1143
98 303.1216 302.1143
99 303.1216 302.1143
100 303.1216 302.1143
101 303.1216 302.1143
102 303.1217 302.1144
103 303.1217 302.1144
104 303.1217 302.1144
105 303.1217 302.1144
106 303.1218 302.1145
107 303.1453 302.1380
108 303.1579 302.1506
109 303.1581 302.1508
110 303.1581 302.1508
111 303.1582 302.1509
112 303.1791 302.1718
113 304.1402 303.1329
114 304.1768 303.1695
115 304.1768 303.1695
116 304.1768 303.1695
117 304.1768 303.1695
118 305.0113 304.0040
119 305.0623 304.0550
120 305.0984 304.0911
121 305.0988 304.0915
122 305.0988 304.0915
123 305.0990 304.0917
124 305.0991 304.0918
125 305.0991 304.0918
126 305.0995 304.0922
127 305.1003 304.0930
128 305.1004 304.0931
129 305.1004 304.0931
130 305.1005 304.0932
131 305.1006 304.0933
132 305.1007 304.0934
133 305.1007 304.0934
134 305.1015 304.0942
135 305.1019 304.0946
136 305.1193 304.1120
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