MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4391)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1537 349.1651 348.1578
1538 349.1656 348.1583
1539 349.1848 348.1775
1540 349.1851 348.1778
1541 349.2000 348.1927
1542 349.2002 348.1929
1543 349.2002 348.1929
1544 349.2002 348.1929
1545 349.2002 348.1929
1546 349.2002 348.1929
1547 349.2002 348.1929
1548 349.2003 348.1930
1549 349.2003 348.1930
1550 349.2003 348.1930
1551 349.2003 348.1930
1552 349.2003 348.1930
1553 349.2003 348.1930
1554 349.2003 348.1930
1555 349.2003 348.1930
1556 349.2004 348.1931
1557 349.2004 348.1931
1558 349.2004 348.1931
1559 349.2005 348.1932
1560 349.2005 348.1932
1561 349.2007 348.1934
1562 349.2197 348.2124
1563 349.2204 348.2131
1564 349.2205 348.2132
1565 349.2211 348.2138
1566 349.2215 348.2142
1567 351.0763 350.0690
1568 351.0767 350.0694
1569 351.1041 350.0968
1570 351.1043 350.0970
1571 351.1046 350.0973
1572 351.1049 350.0976
1573 351.1050 350.0977
1574 351.1053 350.0980
1575 351.1053 350.0980
1576 351.1071 350.0998
1577 351.1087 350.1014
1578 351.1418 350.1345
1579 351.1429 350.1356
1580 351.1429 350.1356
1581 351.1430 350.1357
1582 351.1430 350.1357
1583 351.1431 350.1358
1584 351.1431 350.1358
1585 351.1432 350.1359
1586 351.1432 350.1359
1587 351.1432 350.1359
1588 351.1432 350.1359
1589 351.1432 350.1359
1590 351.1432 350.1359
1591 351.1433 350.1360
1592 351.1433 350.1360
1593 351.1433 350.1360
1594 351.1433 350.1360
1595 351.1435 350.1362
1596 351.1436 350.1363
1597 351.1778 350.1705
1598 351.1795 350.1722
1599 351.1795 350.1722
1600 351.1796 350.1723
1601 351.1796 350.1723
1602 351.1796 350.1723
1603 351.1796 350.1723
1604 351.1796 350.1723
1605 351.1796 350.1723
1606 351.1796 350.1723
1607 351.1796 350.1723
1608 351.1796 350.1723
1609 351.1796 350.1723
1610 351.1796 350.1723
1611 351.1797 350.1724
1612 351.1797 350.1724
1613 351.1797 350.1724
1614 351.1798 350.1725
1615 351.1798 350.1725
1616 351.1799 350.1726
1617 351.1812 350.1739
1618 351.1813 350.1740
1619 351.1815 350.1742
1620 351.1982 350.1909
1621 351.1988 350.1915
1622 351.2159 350.2086
1623 351.2160 350.2087
1624 351.2160 350.2087
1625 351.2160 350.2087
1626 351.2161 350.2088
1627 351.2162 350.2089
1628 351.2163 350.2090
1629 351.2358 350.2285
1630 351.2360 350.2287
1631 351.2361 350.2288
1632 351.2361 350.2288
1633 351.2363 350.2290
1634 351.2367 350.2294
1635 352.2196 351.2123
1636 353.0935 352.0862
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