MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1337 341.1960 340.1887
1338 341.1963 340.1890
1339 341.1964 340.1891
1340 341.1964 340.1891
1341 341.1965 340.1892
1342 341.1966 340.1893
1343 341.1968 340.1895
1344 341.1968 340.1895
1345 341.1972 340.1899
1346 341.1973 340.1900
1347 341.2155 340.2082
1348 341.2165 340.2092
1349 342.1919 341.1846
1350 342.1919 341.1846
1351 343.0963 342.0890
1352 343.0966 342.0893
1353 343.0977 342.0904
1354 343.1160 342.1087
1355 343.1168 342.1095
1356 343.1168 342.1095
1357 343.1169 342.1096
1358 343.1169 342.1096
1359 343.1170 342.1097
1360 343.1172 342.1099
1361 343.1376 342.1303
1362 343.1379 342.1306
1363 343.1383 342.1310
1364 343.1400 342.1327
1365 343.1406 342.1333
1366 343.1415 342.1342
1367 343.1537 342.1464
1368 343.1538 342.1465
1369 343.1538 342.1465
1370 343.1543 342.1470
1371 343.1547 342.1474
1372 343.1547 342.1474
1373 343.1744 342.1671
1374 343.1749 342.1676
1375 343.1749 342.1676
1376 343.1756 342.1683
1377 343.1759 342.1686
1378 343.1916 342.1843
1379 343.7020 342.6947
1380 344.0204 343.0131
1381 344.2080 343.2007
1382 344.2081 343.2008
1383 344.2083 343.2010
1384 344.2084 343.2011
1385 344.2087 343.2014
1386 344.2296 343.2223
1387 345.0280 344.0207
1388 345.0502 344.0429
1389 345.0870 344.0797
1390 345.0890 344.0817
1391 345.0944 344.0871
1392 345.0945 344.0872
1393 345.1135 344.1062
1394 345.1321 344.1248
1395 345.1323 344.1250
1396 345.1323 344.1250
1397 345.1324 344.1251
1398 345.1324 344.1251
1399 345.1324 344.1251
1400 345.1324 344.1251
1401 345.1324 344.1251
1402 345.1324 344.1251
1403 345.1325 344.1252
1404 345.1325 344.1252
1405 345.1326 344.1253
1406 345.1326 344.1253
1407 345.1327 344.1254
1408 345.1544 344.1471
1409 345.1550 344.1477
1410 345.1552 344.1479
1411 345.1553 344.1480
1412 345.1556 344.1483
1413 345.1557 344.1484
1414 345.1564 344.1491
1415 345.1688 344.1615
1416 345.1689 344.1616
1417 345.1689 344.1616
1418 345.1690 344.1617
1419 345.1691 344.1618
1420 345.1691 344.1618
1421 345.1691 344.1618
1422 345.1692 344.1619
1423 345.1693 344.1620
1424 345.1694 344.1621
1425 345.1694 344.1621
1426 345.1696 344.1623
1427 345.1696 344.1623
1428 345.1698 344.1625
1429 345.1920 344.1847
1430 345.2257 344.2184
1431 345.2765 344.2692
1432 346.2237 345.2164
1433 346.2239 345.2166
1434 346.2240 345.2167
1435 346.2241 345.2168
1436 346.2603 345.2530
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