MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1037 333.1326 332.1253
1038 333.1328 332.1255
1039 333.1347 332.1274
1040 333.1686 332.1613
1041 333.1687 332.1614
1042 333.1688 332.1615
1043 333.1688 332.1615
1044 333.1689 332.1616
1045 333.1689 332.1616
1046 333.1689 332.1616
1047 333.1689 332.1616
1048 333.1689 332.1616
1049 333.1689 332.1616
1050 333.1690 332.1617
1051 333.1690 332.1617
1052 333.1690 332.1617
1053 333.1690 332.1617
1054 333.1691 332.1618
1055 333.1692 332.1619
1056 333.1693 332.1620
1057 333.1693 332.1620
1058 333.1700 332.1627
1059 333.2050 332.1977
1060 333.2050 332.1977
1061 333.2050 332.1977
1062 333.2050 332.1977
1063 333.2051 332.1978
1064 333.2051 332.1978
1065 333.2051 332.1978
1066 333.2052 332.1979
1067 333.2052 332.1979
1068 333.2053 332.1980
1069 333.2053 332.1980
1070 333.2053 332.1980
1071 333.2056 332.1983
1072 333.2259 332.2186
1073 333.2260 332.2187
1074 333.2263 332.2190
1075 334.1343 333.1270
1076 334.1346 333.1273
1077 334.1507 333.1434
1078 334.1525 333.1452
1079 334.1728 333.1655
1080 334.1729 333.1656
1081 334.1874 333.1801
1082 334.1877 333.1804
1083 334.2238 333.2165
1084 334.2240 333.2167
1085 335.0728 334.0655
1086 335.0906 334.0833
1087 335.1092 334.1019
1088 335.1093 334.1020
1089 335.1096 334.1023
1090 335.1103 334.1030
1091 335.1103 334.1030
1092 335.1105 334.1032
1093 335.1112 334.1039
1094 335.1113 334.1040
1095 335.1115 334.1042
1096 335.1116 334.1043
1097 335.1116 334.1043
1098 335.1116 334.1043
1099 335.1118 334.1045
1100 335.1188 334.1115
1101 335.1459 334.1386
1102 335.1480 334.1407
1103 335.1480 334.1407
1104 335.1480 334.1407
1105 335.1481 334.1408
1106 335.1481 334.1408
1107 335.1481 334.1408
1108 335.1482 334.1409
1109 335.1482 334.1409
1110 335.1482 334.1409
1111 335.1482 334.1409
1112 335.1482 334.1409
1113 335.1482 334.1409
1114 335.1482 334.1409
1115 335.1483 334.1410
1116 335.1483 334.1410
1117 335.1483 334.1410
1118 335.1484 334.1411
1119 335.1484 334.1411
1120 335.1484 334.1411
1121 335.1485 334.1412
1122 335.1501 334.1428
1123 335.1844 334.1771
1124 335.1845 334.1772
1125 335.1845 334.1772
1126 335.1845 334.1772
1127 335.1846 334.1773
1128 335.1847 334.1774
1129 335.1847 334.1774
1130 335.1847 334.1774
1131 335.1847 334.1774
1132 335.1848 334.1775
1133 335.1848 334.1775
1134 335.1848 334.1775
1135 335.1848 334.1775
1136 335.1848 334.1775
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