MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4391)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
837 325.1640 324.1567
838 325.1641 324.1568
839 325.1641 324.1568
840 325.1641 324.1568
841 325.1641 324.1568
842 325.1642 324.1569
843 325.1653 324.1580
844 325.1654 324.1581
845 325.1656 324.1583
846 325.1657 324.1584
847 325.1822 324.1749
848 325.1825 324.1752
849 325.1831 324.1758
850 325.2010 324.1937
851 325.2206 324.2133
852 326.1474 325.1401
853 326.1672 325.1599
854 326.1852 325.1779
855 326.1971 325.1898
856 326.1972 325.1899
857 327.0409 326.0336
858 327.0764 326.0691
859 327.0765 326.0692
860 327.0768 326.0695
861 327.0801 326.0728
862 327.0860 326.0787
863 327.0871 326.0798
864 327.1043 326.0970
865 327.1055 326.0982
866 327.1058 326.0985
867 327.1060 326.0987
868 327.1063 326.0990
869 327.1068 326.0995
870 327.1081 326.1008
871 327.1218 326.1145
872 327.1221 326.1148
873 327.1221 326.1148
874 327.1233 326.1160
875 327.1321 326.1248
876 327.1325 326.1252
877 327.1422 326.1349
878 327.1424 326.1351
879 327.1429 326.1356
880 327.1429 326.1356
881 327.1429 326.1356
882 327.1432 326.1359
883 327.1432 326.1359
884 327.1434 326.1361
885 327.1435 326.1362
886 327.1436 326.1363
887 327.1436 326.1363
888 327.1436 326.1363
889 327.1436 326.1363
890 327.1438 326.1365
891 327.1439 326.1366
892 327.1446 326.1373
893 327.1451 326.1378
894 327.1793 326.1720
895 327.1794 326.1721
896 327.1794 326.1721
897 327.1794 326.1721
898 327.1795 326.1722
899 327.1796 326.1723
900 327.1796 326.1723
901 327.1796 326.1723
902 327.1797 326.1724
903 327.1797 326.1724
904 327.1797 326.1724
905 327.1797 326.1724
906 327.1798 326.1725
907 327.1798 326.1725
908 327.1990 326.1917
909 327.1996 326.1923
910 327.1998 326.1925
911 327.2001 326.1928
912 327.2002 326.1929
913 327.2003 326.1930
914 327.2027 326.1954
915 327.2174 326.2101
916 327.2176 326.2103
917 328.2131 327.2058
918 328.2501 327.2428
919 329.0207 328.0134
920 329.0552 328.0479
921 329.0821 328.0748
922 329.0915 328.0842
923 329.1216 328.1143
924 329.1216 328.1143
925 329.1217 328.1144
926 329.1251 328.1178
927 329.1369 328.1296
928 329.1375 328.1302
929 329.1377 328.1304
930 329.1377 328.1304
931 329.1378 328.1305
932 329.1378 328.1305
933 329.1379 328.1306
934 329.1388 328.1315
935 329.1582 328.1509
936 329.1584 328.1511
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