MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4391)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
737 323.1113 322.1040
738 323.1114 322.1041
739 323.1114 322.1041
740 323.1115 322.1042
741 323.1116 322.1043
742 323.1116 322.1043
743 323.1116 322.1043
744 323.1117 322.1044
745 323.1127 322.1054
746 323.1133 322.1060
747 323.1468 322.1395
748 323.1477 322.1404
749 323.1479 322.1406
750 323.1479 322.1406
751 323.1479 322.1406
752 323.1479 322.1406
753 323.1479 322.1406
754 323.1480 322.1407
755 323.1480 322.1407
756 323.1480 322.1407
757 323.1480 322.1407
758 323.1481 322.1408
759 323.1481 322.1408
760 323.1481 322.1408
761 323.1482 322.1409
762 323.1482 322.1409
763 323.1483 322.1410
764 323.1483 322.1410
765 323.1487 322.1414
766 323.1497 322.1424
767 323.1498 322.1425
768 323.1654 322.1581
769 323.1658 322.1585
770 323.1662 322.1589
771 323.1664 322.1591
772 323.1677 322.1604
773 323.1847 322.1774
774 323.1847 322.1774
775 323.1847 322.1774
776 323.1847 322.1774
777 323.1848 322.1775
778 323.1848 322.1775
779 323.1848 322.1775
780 323.1848 322.1775
781 323.1848 322.1775
782 323.1848 322.1775
783 323.1849 322.1776
784 323.1849 322.1776
785 323.1850 322.1777
786 323.1851 322.1778
787 323.1851 322.1778
788 323.1852 322.1779
789 323.1855 322.1782
790 323.1862 322.1789
791 323.1862 322.1789
792 323.2039 322.1966
793 323.2045 322.1972
794 324.1880 323.1807
795 325.0633 324.0560
796 325.0870 324.0797
797 325.0883 324.0810
798 325.0898 324.0825
799 325.0914 324.0841
800 325.0915 324.0842
801 325.0921 324.0848
802 325.0973 324.0900
803 325.1064 324.0991
804 325.1064 324.0991
805 325.1065 324.0992
806 325.1074 324.1001
807 325.1264 324.1191
808 325.1270 324.1197
809 325.1270 324.1197
810 325.1270 324.1197
811 325.1270 324.1197
812 325.1270 324.1197
813 325.1271 324.1198
814 325.1272 324.1199
815 325.1272 324.1199
816 325.1273 324.1200
817 325.1273 324.1200
818 325.1274 324.1201
819 325.1274 324.1201
820 325.1275 324.1202
821 325.1276 324.1203
822 325.1278 324.1205
823 325.1278 324.1205
824 325.1339 324.1266
825 325.1344 324.1271
826 325.1346 324.1273
827 325.1444 324.1371
828 325.1450 324.1377
829 325.1625 324.1552
830 325.1630 324.1557
831 325.1634 324.1561
832 325.1637 324.1564
833 325.1638 324.1565
834 325.1639 324.1566
835 325.1639 324.1566
836 325.1639 324.1566
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