MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4391)

Page: 1 2 3 4 5 6 … 44 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4235 510.2821 1018.5496 1018.5447 4.85 1 21 0.092 +1Score > 23 indicates identity FASPNKNLK + Deamidated (NQ)
4163 433.2446 864.4746 864.4705 4.78 0 20 0.054 +1Score > 20 indicates identity
Score > 20 indicates homology		 LVTTFQR + Deamidated (NQ)
4129 404.2061 806.3976 806.3923 6.68 0 19 0.15 +1Score > 24 indicates identity LAADDFR
4242 517.2646 1032.5146 1032.5087 5.73 0 18 0.27 +1Score > 24 indicates identity TLLEGEESR
4019 316.6824 631.3502 631.3475 4.27 0 17 0.31 +1Score > 25 indicates identity LCIAR
4283 559.3245 1116.6344 1116.6291 4.76 1 17 0.16 +1Score > 21 indicates identity VPGRDGLLYK
4174 444.2531 886.4916 886.4984 -7.67 1 17 0.18 +1Score > 25 indicates identity
Score > 22 indicates homology		 AVDALRSR
4145 420.7526 839.4906 839.4865 4.96 1 17 0.072 +1Score > 18 indicates identity KTIEPPR
3933 502.3260 501.3187 501.3275 -17.4 1 16 0.46 +1Score > 26 indicates identity KGGLK
4120 400.7494 799.4842 799.4803 4.90 0 16 0.18 +1Score > 21 indicates identity LADAIVAK
4295 576.3276 1150.6406 1150.6346 5.26 0 16 0.085 +1Score > 20 indicates identity
Score > 17 indicates homology		 LIIAGTSAYSR
4103 388.7204 775.4262 775.4228 4.42 0 15 0.11 +1Score > 22 indicates identity
Score > 18 indicates homology		 LIDYPR
4387 398.9645 1591.8289 1591.7994 18.5 1 15 0.39 +1Score > 23 indicates identity NNPKELTVYFGGPR + Deamidated (NQ)
4111 394.7128 787.4110 787.4076 4.43 0 15 0.2 +1Score > 25 indicates identity
Score > 20 indicates homology		 GGNELLGK + Deamidated (NQ)
4013 628.4057 627.3984 627.3956 4.57 0 14 0.14 +1Score > 18 indicates identity TVIPAK
4223 500.7589 999.5032 999.4920 11.3 1 14 0.09 +1Score > 22 indicates identity
Score > 16 indicates homology		 MVNEHRAK + Oxidation (M)
4014 314.7066 627.3986 627.3956 4.93 0 14 0.16 +1Score > 18 indicates identity TVIPAK
4325 632.2986 1262.5826 1262.5739 6.97 1 13 0.081 +1Score > 23 indicates identity
Score > 15 indicates homology		 NASGQDTGLKDR + 2 Deamidated (NQ)
4173 443.7552 885.4958 885.4920 4.39 0 13 0.65 +1Score > 23 indicates identity AQLEVVAR + Deamidated (NQ)
4122 401.2343 800.4540 800.4505 4.49 0 12 0.47 +1Score > 25 indicates identity
Score > 22 indicates homology		 GLATGVQR
4274 549.8320 1097.6494 1097.6444 4.56 0 12 0.28 +1Score > 19 indicates identity IPASDTLIIR
4358 691.3307 1380.6468 1380.6521 -3.78 1 12 0.72 -1Score > 23 indicates identity ALQSQREYEEK + Deamidated (NQ)
4088 372.7180 743.4214 743.4177 5.01 0 12 0.92 +1Score > 24 indicates identity
Score > 24 indicates homology		 QNELLK
3987 586.3953 585.3880 585.3962 -14.0 1 12 0.56 +1Score > 22 indicates identity KVIAR
4127 402.6893 803.3640 803.3524 14.6 0 12 0.49 +1Score > 22 indicates identity
Score > 21 indicates homology		 EYAFMK + Oxidation (M)
4234 509.7643 1017.5140 1017.4978 16.0 0 12 0.14 +1Score > 25 indicates identity
Score > 16 indicates homology		 TIANDNEIK + Deamidated (NQ)
4300 386.5577 1156.6513 1156.6452 5.29 1 12 0.32 +1Score > 23 indicates identity
Score > 19 indicates homology		 GAKENLDAIVK
4042 329.6994 657.3842 657.3810 5.02 0 11 0.38 +1Score > 24 indicates identity
Score > 20 indicates homology		 ALLDAR
4044 330.1970 658.3794 658.3762 4.90 0 11 0.42 +1Score > 25 indicates identity
Score > 20 indicates homology		 GIAVGSR
4126 402.6891 803.3636 803.3524 14.1 0 11 0.66 +1Score > 22 indicates identity
Score > 22 indicates homology		 EYAFMK + Oxidation (M)
4092 374.2314 746.4482 746.4439 5.83 0 11 0.28 +1Score > 18 indicates identity ALQLFR
3997 306.6964 611.3782 611.3867 -13.8 1 11 0.23 +1Score > 17 indicates identity ARLPR
4249 522.8087 1043.6028 1043.5863 15.9 1 11 0.1 +1Score > 22 indicates identity
Score > 14 indicates homology		 TTPLEEVKK
4153 426.2188 850.4230 850.4297 -7.81 1 11 0.18 +1Score > 24 indicates identity
Score > 16 indicates homology		 YADQAKR
4076 360.7257 719.4368 719.4442 -10.2 1 11 0.17 +1Score > 16 indicates identity YIRIR
4378 768.8901 1535.7656 1535.7654 0.18 1 11 0.18 +1Score > 24 indicates identity
Score > 16 indicates homology		 SEMWEVTNKAVVK + Oxidation (M)
4140 415.7423 829.4700 829.4657 5.19 0 11 0.72 +1Score > 25 indicates identity
Score > 22 indicates homology		 AVNSLGLR + Deamidated (NQ)
4158 429.7398 857.4650 857.4759 -12.7 0 11 0.5 +1Score > 24 indicates identity
Score > 20 indicates homology		 IVAWAGNK
4086 743.4442 742.4369 742.4337 4.30 0 10 1 +1Score > 24 indicates identity
Score > 23 indicates homology		 ALVTSPR
4104 389.2495 776.4844 776.4796 6.24 1 10 0.13 +1Score > 14 indicates identity IIFEKK
4090 373.2282 744.4418 744.4381 5.00 0 10 0.63 +1Score > 24 indicates identity
Score > 20 indicates homology		 LAATLEK
4190 459.7681 917.5216 917.5294 -8.45 1 10 0.17 +1Score > 20 indicates identity
Score > 14 indicates homology		 SSGLISKAR
4210 482.2528 962.4910 962.4855 5.74 0 10 1 +1Score > 24 indicates identity
Score > 22 indicates homology		 LGTPAMTTR + Oxidation (M)
4178 449.7918 897.5690 897.5760 -7.70 1 9 0.22 +1Score > 15 indicates identity IIIIDRR
4094 375.2157 748.4168 748.4119 6.56 0 9 0.49 +1Score > 22 indicates identity
Score > 19 indicates homology		 AGVFDLK
4170 437.7495 873.4844 873.4708 15.6 0 9 0.49 +1Score > 25 indicates identity
Score > 19 indicates homology		 INLPDFR
4107 393.2495 784.4844 784.4807 4.80 0 9 0.53 +1Score > 21 indicates identity
Score > 19 indicates homology		 LAQVQVK
4269 544.7916 1087.5686 1087.5509 16.3 0 9 0.29 +1Score > 25 indicates identity
Score > 16 indicates homology		 NDLELNTLR + Deamidated (NQ)
4133 407.7447 813.4748 813.4708 4.95 0 9 0.49 +1Score > 23 indicates identity
Score > 18 indicates homology		 IVAELNR
4293 576.2852 1150.5558 1150.5466 8.08 1 9 1 +1Score > 24 indicates identity
Score > 21 indicates homology		 SQQSNTSLKR + 3 Deamidated (NQ)
4342 652.3495 1302.6844 1302.6667 13.6 0 9 1 +1Score > 24 indicates identity
Score > 21 indicates homology		 QVVDTTVDLGQK + Deamidated (NQ)
4280 372.5419 1114.6039 1114.6168 -11.6 1 9 0.92 +1Score > 23 indicates identity
Score > 21 indicates homology		 KPGSMNLPKK + Oxidation (M)
4171 439.7427 877.4708 877.4691 1.96 1 9 0.66 +1Score > 23 indicates identity
Score > 19 indicates homology		 IKVTDMR + Oxidation (M)
4134 409.2261 816.4376 816.4341 4.33 0 8 1 +1Score > 25 indicates identity
Score > 21 indicates homology		 GINSGQIK + Deamidated (NQ)
4066 351.1836 700.3526 700.3504 3.23 1 8 0.77 +1Score > 21 indicates identity
Score > 20 indicates homology		 DDKAPR
4000 308.1833 614.3520 614.3500 3.35 0 8 1 +1Score > 22 indicates identity
Score > 21 indicates homology		 GAQALR
4123 401.7221 801.4296 801.4344 -5.97 1 8 0.99 +1Score > 25 indicates identity
Score > 20 indicates homology		 LAQAKDR + Deamidated (NQ)
4125 402.2184 802.4222 802.4185 4.71 0 8 0.25 +1Score > 26 indicates identity
Score > 14 indicates homology		 TGEIIDR
4309 403.8844 1208.6314 1208.6262 4.29 0 8 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 ISVSPQTGQHR
4368 725.3293 1448.6440 1448.6379 4.22 0 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology		 EDGTLDNTQSVDR
4143 416.7443 831.4740 831.4636 12.5 0 8 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 LMRPSTK
4412 749.0679 2244.1819 2244.1686 5.90 1 7 0.72 +1Score > 21 indicates identity
Score > 18 indicates homology		 CNLLAPWFIDTPLIAPMKK + Deamidated (NQ); Oxidation (M)
4216 489.2765 976.5384 976.5454 -7.11 1 7 1 +1Score > 22 indicates identity
Score > 20 indicates homology		 FASIEVRR
4442 987.8633 2960.5681 2960.5220 15.5 0 7 1 +1Score > 20 indicates identity
Score > 20 indicates homology		 MFTIFVFMFVAPGVVNQLQPLFIER + 2 Deamidated (NQ); Oxidation (M)
4080 364.7258 727.4370 727.4341 4.10 0 7 0.34 +1Score > 20 indicates identity
Score > 15 indicates homology		 IGVAVNR
4319 414.9208 1241.7406 1241.7244 13.0 1 7 0.49 +1Score > 16 indicates identity WVLRSDLVVR
4192 461.2576 920.5006 920.4868 15.0 1 7 1 +1Score > 23 indicates identity
Score > 20 indicates homology		 FAVRDWK
4268 363.1996 1086.5770 1086.5557 19.6 0 7 1 +1Score > 24 indicates identity
Score > 19 indicates homology		 EVQAIQEAAK + Deamidated (NQ)
4225 501.7973 1001.5800 1001.5692 10.9 1 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology		 KMIQLVDR
4054 337.2172 672.4198 672.4170 4.20 0 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 VSIIGGK
4046 330.6786 659.3426 659.3391 5.39 0 7 0.68 +1Score > 25 indicates identity
Score > 18 indicates homology		 WVEAR
4326 421.8687 1262.5843 1262.5813 2.38 0 7 0.29 +1Score > 23 indicates identity
Score > 14 indicates homology		 EGGDLVPGAMSSK + Oxidation (M)
4278 372.1954 1113.5644 1113.5495 13.4 1 7 0.37 +1Score > 22 indicates identity
Score > 15 indicates homology		 DFYNIKWK + Deamidated (NQ)
4142 416.2469 830.4792 830.4862 -8.32 0 7 0.4 +1Score > 22 indicates identity
Score > 15 indicates homology		 ITQVITR + Deamidated (NQ)
4307 600.8372 1199.6598 1199.6636 -3.09 0 7 0.25 +1Score > 22 indicates identity
Score > 13 indicates homology		 EGRPRPPHVR
4252 523.7579 1045.5012 1045.5152 -13.3 1 7 0.38 +1Score > 23 indicates identity
Score > 15 indicates homology		 SANGRLDANK + Deamidated (NQ)
4256 527.2562 1052.4978 1052.4927 4.91 0 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology		 FAQYQAPAR + 2 Deamidated (NQ)
4108 393.7291 785.4436 785.4395 5.22 0 6 1 +1Score > 23 indicates identity
Score > 19 indicates homology		 LGKPTDR
4232 508.7787 1015.5428 1015.5549 -11.9 1 6 0.41 +1Score > 24 indicates identity
Score > 15 indicates homology		 QIKELEEK
4096 380.2003 758.3860 758.3922 -8.17 0 6 0.42 +1Score > 23 indicates identity
Score > 15 indicates homology		 QNQLQK + Deamidated (NQ)
4308 401.5370 1201.5892 1201.5687 17.0 1 6 0.44 +1Score > 24 indicates identity
Score > 15 indicates homology		 REDLEEQQR
4231 508.7737 1015.5328 1015.5338 -0.97 1 6 1 +1Score > 24 indicates identity
Score > 18 indicates homology		 KANDVPFPK + Deamidated (NQ)
4075 358.7207 715.4268 715.4228 5.63 0 5 0.78 +1Score > 22 indicates identity
Score > 17 indicates homology		 IQSVLR + Deamidated (NQ)
4392 554.5992 1660.7758 1660.8056 -18.0 1 5 0.58 +1Score > 23 indicates identity
Score > 15 indicates homology		 EDSFDLTPLSPRER
4067 351.2211 700.4276 700.4232 6.41 1 5 0.51 +1Score > 22 indicates identity
Score > 15 indicates homology		 LGKGAQK
4102 388.6896 775.3646 775.3759 -14.5 1 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 VRCDAR
4022 318.1816 634.3486 634.3472 2.24 0 5 1 +1Score > 22 indicates identity
Score > 18 indicates homology		 VMTIR + Oxidation (M)
4214 487.2734 972.5322 972.5505 -18.8 0 5 0.55 +1Score > 24 indicates identity
Score > 15 indicates homology		 TPLHHAVAK
4135 411.2021 820.3896 820.3926 -3.65 1 5 1 +1Score > 23 indicates identity
Score > 17 indicates homology		 KTQNDSK + Deamidated (NQ)
4189 458.2814 914.5482 914.5549 -7.27 1 5 1 +1Score > 20 indicates identity
Score > 17 indicates homology		 KAVIVTER
4238 515.2554 1028.4962 1028.4814 14.4 0 5 1 +1Score > 22 indicates identity
Score > 17 indicates homology		 INNASFSFK + 2 Deamidated (NQ)
4011 314.6756 627.3366 627.3340 4.22 0 5 1.7 +1Score > 19 indicates identity IENPR
4433 917.5069 2749.4989 2749.4585 14.7 0 4 1.1 +1Score > 17 indicates identity LPANRPMLTELQIPAVHSSLLDYR + Oxidation (M)
4380 386.4550 1541.7909 1541.8049 -9.10 0 4 0.59 +1Score > 24 indicates identity
Score > 15 indicates homology		 GVSPLTQAQISEGVR + Deamidated (NQ)
4182 453.7232 905.4318 905.4355 -4.03 1 4 1 +1Score > 23 indicates identity
Score > 17 indicates homology		 NKYDPNR
4337 643.3629 1284.7112 1284.7190 -6.03 1 4 0.5 +1Score > 22 indicates identity
Score > 14 indicates homology		 AGKVLAYAIHDK
4222 496.7766 991.5386 991.5372 1.47 1 4 1 +1Score > 22 indicates identity
Score > 17 indicates homology		 EILKQMSK + Oxidation (M)
4150 423.2293 844.4440 844.4290 17.8 0 4 0.92 +1Score > 25 indicates identity
Score > 16 indicates homology		 IGEQLQR + 2 Deamidated (NQ)
4303 593.8223 1185.6300 1185.6241 5.00 0 4 1 +1Score > 23 indicates identity
Score > 16 indicates homology		 SPEVASVPVSSK
4130 404.2420 806.4694 806.4650 5.46 0 4 2 +1Score > 20 indicates identity VVLGYTR
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