MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1231_JBEI_2.mgf |
| Database | : | A-oryzae 20191108 (12,205 sequences; 5,465,702 residues) |
| Timestamp | : | 1 May 2025 at 20:39:04 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| Score | Mass | Matches | Sequences | emPAI | |||
| 1 | IGF1_BOVIN | 172 | 42206 | 22 (10) | 16 (8) | 1.13 | Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal |
| 2 | Q2UJR1_ASPOR | 137 | 74750 | 14 (7) | 13 (7) | 0.35 | Acetyl-coenzyme A synthetase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003001112 PE=3 SV=1 |
| 3 | TRYP_PIG | 133 | 25078 | 5 (5) | 3 (3) | 0.65 | Trypsin OS=Sus scrofa PE=1 SV=1 |
| 4 | Q2UK92_ASPOR | 36 | 63866 | 3 (2) | 3 (2) | 0.11 | AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1 |
| 5 | Q2U1V7_ASPOR | 34 | 35502 | 1 (1) | 1 (1) | 0.09 | ANK_REP_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090138000080 PE=4 SV=1 |
| 6 | Q2UKB4_ASPOR | 31 | 91359 | 2 (1) | 1 (1) | 0.04 | Urease OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000879 PE=3 SV=1 |
| 7 | Q2U034_ASPOR | 30 | 131681 | 3 (1) | 3 (1) | 0.02 | SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1 |
| 8 | Q2UHF6_ASPOR | 27 | 54290 | 1 (1) | 1 (1) | 0.06 | Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1 |
| 9 | Q2UK62_ASPOR | 25 | 75289 | 1 (1) | 1 (1) | 0.04 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000939 PE=4 SV=1 |
| 10 | PEPA_ASPOR | 25 | 0 | 1 (1) | 1 (1) | 0.07 | description |
| 11 | Q2U2M3_ASPOR | 23 | 71213 | 1 (1) | 1 (1) | 0.05 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090038000399 PE=4 SV=1 |
| 12 | Q2U9S4_ASPOR | 23 | 25368 | 1 (1) | 1 (1) | 0.13 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090166000038 PE=4 SV=1 |
| 13 | Q2U0S3_ASPOR | 22 | 0 | 1 (1) | 1 (1) | 0.03 | description |
| 14 | Q2UAS1_ASPOR | 19 | 0 | 1 (1) | 1 (1) | 0.05 | description |
| 15 | Q2U9Y9_ASPOR | 19 | 0 | 1 (1) | 1 (1) | 0.04 | description |
| 16 | Q2UTE7_ASPOR | 19 | 32098 | 1 (1) | 1 (1) | 0.10 | Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090005000048 PE=3 SV=1 |
| 17 | Q2U650_ASPOR | 18 | 43022 | 1 (1) | 1 (1) | 0.08 | Aldo_ket_red domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090120000379 PE=4 SV=1 |
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