MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 17)

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+1

Accession Score Description
1 IGF1_BOVIN 172 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

+2

Accession Score Description
1 Q2UJR1_ASPOR 137 Acetyl-coenzyme A synthetase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003001112 PE=3 SV=1

-3

Accession Score Description
1 TRYP_PIG 133 Trypsin OS=Sus scrofa PE=1 SV=1
Score Mass Matches Sequences emPAI
3.1 TRYP_PIG 133 25078 5 (5) 3 (3) 0.65
Trypsin OS=Sus scrofa PE=1 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4148 +1 421.7604 841.5062 841.5022 4.87 0 55 1.9e-005 +1Score > 20 indicates identity U R.VATVSLPR.S
4250   523.2876 1044.5606 1044.5564 4.11 0 45 0.00045 +1Score > 24 indicates identity U K.LSSPATLNSR.V
4253   523.7820 1045.5494 1045.5404 8.69 0 28 0.0093 +1Score > 25 indicates identity
Score > 20 indicates homology		 U K.LSSPATLNSR.V + Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_2.raw

Score > 23 indicates identity

Score > 17 indicates homology
4411   738.0435 2211.1087 2211.0807 12.6 0 41 0.00022 -1Score > 23 indicates identity
Score > 17 indicates homology		 U R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ)
12.6 0 41 0.00022 1 R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ)
12.6 0 22 0.018 3 R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ)
12.6 0 3 1.4 4 R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ)
16.7 1 2 1.7 5 LPNGMPFGHRLWCEIQVR   + 2 Deamidated (NQ)

+4

Accession Score Description
1 Q2UK92_ASPOR 36 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+5

Accession Score Description
1 Q2U1V7_ASPOR 34 ANK_REP_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090138000080 PE=4 SV=1

+6

Accession Score Description
1 Q2UKB4_ASPOR 31 Urease OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000879 PE=3 SV=1

+7

Accession Score Description
1 Q2U034_ASPOR 30 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1

+8

Accession Score Description
1 Q2UHF6_ASPOR 27 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+9

Accession Score Description
1 Q2UK62_ASPOR 25 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000939 PE=4 SV=1

+10

Accession Score Description
1 PEPA_ASPOR 25 description
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