MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1231_JBEI_2.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:39:04 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 17)

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+1

Accession Score Description
1 IGF1_BOVIN 172 Insulin-like growth factor 1 OS=Bos taurus OX=9913 GN=IGF1 PE=1 SV=2 Gly50-Ala119 Histag TEV fused with GFP with secretion signal

-2

Accession Score Description
1 Q2UJR1_ASPOR 137 Acetyl-coenzyme A synthetase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003001112 PE=3 SV=1
Score Mass Matches Sequences emPAI
2.1 Q2UJR1_ASPOR 137 74750 14 (7) 13 (7) 0.35
Acetyl-coenzyme A synthetase OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003001112 PE=3 SV=1

-14 peptide matches (14 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
4063   345.1962 688.3778 688.3755 3.34 0 19 0.059 +1Score > 26 indicates identity
Score > 19 indicates homology		 U R.VIDAGSK.V
4074   358.2310 714.4474 714.4428 6.45 0 18 0.12 +1Score > 23 indicates identity
Score > 21 indicates homology		 U R.VAWVLK.Q
4110   394.2336 786.4526 786.4487 5.02 0 18 0.084 +1Score > 24 indicates identity
Score > 19 indicates homology		 U R.IVDEALK.Q
4177   448.2320 894.4494 894.4447 5.34 0 27 0.0081 +1Score > 22 indicates identity
Score > 19 indicates homology		 U K.LYDESIR.S
D:\Xcalibur\Data\Jennifer\20250430 PRT1231 Ofer\PRT1231_JBEI_2.raw

Score > 23 indicates identity
4205   476.7448 951.4750 951.4702 5.13 0 18 0.15 -1Score > 23 indicates identity U R.YWDVIEK.H
No other peptide matches in query
4208   481.2659 960.5172 960.5128 4.64 0 2 1 +1Score > 24 indicates identity
Score > 14 indicates homology		 U K.VVITTDEGK.R
4221   496.7534 991.4922 991.4876 4.72 0 36 0.0007 +1Score > 24 indicates identity
Score > 17 indicates homology		 U R.SPQTFWAR.M
4260   533.3034 1064.5922 1064.5866 5.31 0 22 0.032 +1Score > 19 indicates identity U R.TITYGELLR.E
4281   373.2136 1116.6190 1116.6139 4.55 1 30 0.0096 +1Score > 23 indicates identity U K.VVITTDEGKR.G
4282   559.3168 1116.6190 1116.6139 4.62 1 10 0.96 +1Score > 23 indicates identity U K.VVITTDEGKR.G
4306   598.2825 1194.5504 1194.5451 4.45 0 39 0.00028 +1Score > 23 indicates identity
Score > 16 indicates homology		 U R.LNAAYNCVDR.H
4315   616.3100 1230.6054 1230.5993 4.98 0 38 0.001 +1Score > 24 indicates identity
Score > 21 indicates homology		 U R.TGTEVPWTQGR.D
4361   704.8641 1407.7136 1407.7068 4.85 0 11 0.1 +1Score > 23 indicates identity
Score > 14 indicates homology		 U K.QCPDVTSVLVYK.R
4386   788.3877 1574.7608 1574.7576 2.05 0 60 4.8e-006 +1Score > 24 indicates identity
Score > 20 indicates homology		 U K.VAIIYEADEPNEGR.T

+3

Accession Score Description
1 TRYP_PIG 133 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 Q2UK92_ASPOR 36 AlcB domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000906 PE=4 SV=1

+5

Accession Score Description
1 Q2U1V7_ASPOR 34 ANK_REP_REGION domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090138000080 PE=4 SV=1

+6

Accession Score Description
1 Q2UKB4_ASPOR 31 Urease OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000879 PE=3 SV=1

+7

Accession Score Description
1 Q2U034_ASPOR 30 SH3 domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090011000606 PE=4 SV=1

+8

Accession Score Description
1 Q2UHF6_ASPOR 27 Aldedh domain-containing protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090023000467 PE=3 SV=1

+9

Accession Score Description
1 Q2UK62_ASPOR 25 Uncharacterized protein OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=AO090003000939 PE=4 SV=1

+10

Accession Score Description
1 PEPA_ASPOR 25 description
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