MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
376 319.2263 318.2190
377 319.2263 318.2190
378 319.2264 318.2191
379 319.2264 318.2191
380 320.9012 319.8939
381 320.9012 319.8939
382 320.9012 319.8939
383 320.9012 319.8939
384 320.9012 319.8939
385 320.9012 319.8939
386 320.9013 319.8940
387 320.9013 319.8940
388 320.9013 319.8940
389 320.9013 319.8940
390 320.9014 319.8941
391 320.9014 319.8941
392 320.9014 319.8941
393 320.9014 319.8941
394 320.9015 319.8942
395 320.9015 319.8942
396 320.9016 319.8943
397 320.9016 319.8943
398 320.9017 319.8944
399 320.9018 319.8945
400 320.9019 319.8946
401 320.9019 319.8946
402 320.9827 319.9754
403 320.9827 319.9754
404 320.9829 319.9756
405 320.9829 319.9756
406 320.9830 319.9757
407 321.1533 320.1460
408 321.1685 320.1612
409 321.1685 320.1612
410 321.1685 320.1612
411 321.1686 320.1613
412 321.1686 320.1613
413 321.1686 320.1613
414 321.1686 320.1613
415 321.1686 320.1613
416 321.1687 320.1614
417 321.1687 320.1614
418 321.1688 320.1615
419 321.1688 320.1615
420 321.1688 320.1615
421 321.1689 320.1616
422 321.1689 320.1616
423 321.1689 320.1616
424 321.1689 320.1616
425 321.1690 320.1617
426 321.1690 320.1617
427 321.1692 320.1619
428 321.1693 320.1620
429 321.2049 320.1976
430 321.2055 320.1982
431 321.2415 320.2342
432 321.2420 320.2347
433 321.2421 320.2348
434 321.2421 320.2348
435 321.2422 320.2349
436 321.2625 320.2552
437 321.8658 320.8585
438 321.8658 320.8585
439 321.8667 320.8594
440 322.8899 321.8826
441 322.8899 321.8826
442 323.0208 322.0135
443 323.0211 322.0138
444 323.0212 322.0139
445 323.0214 322.0141
446 323.0214 322.0141
447 323.0214 322.0141
448 323.0215 322.0142
449 323.0215 322.0142
450 323.0215 322.0142
451 323.0215 322.0142
452 323.0216 322.0143
453 323.0217 322.0144
454 323.0217 322.0144
455 323.0219 322.0146
456 323.1477 322.1404
457 323.1478 322.1405
458 323.1480 322.1407
459 323.1661 322.1588
460 323.1662 322.1589
461 323.1663 322.1590
462 323.1664 322.1591
463 323.1664 322.1591
464 323.1664 322.1591
465 323.1665 322.1592
466 323.1665 322.1592
467 323.1665 322.1592
468 323.1666 322.1593
469 323.1667 322.1594
470 323.1841 322.1768
471 323.1842 322.1769
472 323.1843 322.1770
473 323.1843 322.1770
474 323.1844 322.1771
475 323.1844 322.1771
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