MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4447)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
276 311.0276 310.0203
277 311.0279 310.0206
278 311.0280 310.0207
279 311.0280 310.0207
280 311.0281 310.0208
281 311.0281 310.0208
282 311.0282 310.0209
283 311.0282 310.0209
284 311.0282 310.0209
285 311.0283 310.0210
286 311.0283 310.0210
287 311.0283 310.0210
288 311.0284 310.0211
289 311.0284 310.0211
290 311.0284 310.0211
291 311.0285 310.0212
292 311.0285 310.0212
293 311.0285 310.0212
294 311.0285 310.0212
295 311.0285 310.0212
296 311.0286 310.0213
297 311.0287 310.0214
298 311.0287 310.0214
299 311.0287 310.0214
300 311.0287 310.0214
301 311.0287 310.0214
302 311.0288 310.0215
303 311.0289 310.0216
304 311.0289 310.0216
305 311.0289 310.0216
306 311.0289 310.0216
307 311.0290 310.0217
308 311.0291 310.0218
309 311.1295 310.1222
310 311.1295 310.1222
311 311.1298 310.1225
312 311.1475 310.1402
313 311.1476 310.1403
314 311.1479 310.1406
315 311.1664 310.1591
316 311.1664 310.1591
317 311.1664 310.1591
318 311.1665 310.1592
319 311.1666 310.1593
320 311.1666 310.1593
321 311.1667 310.1594
322 311.1667 310.1594
323 311.1667 310.1594
324 311.1669 310.1596
325 311.1670 310.1597
326 311.2054 310.1981
327 311.2054 310.1981
328 311.2229 310.2156
329 311.2229 310.2156
330 311.2229 310.2156
331 311.2230 310.2157
332 311.2405 310.2332
333 311.9448 310.9375
334 311.9451 310.9378
335 311.9453 310.9380
336 311.9454 310.9381
337 311.9456 310.9383
338 312.1322 311.1249
339 313.1454 312.1381
340 313.1456 312.1383
341 313.1463 312.1390
342 313.1831 312.1758
343 313.1831 312.1758
344 313.1843 312.1770
345 313.1848 312.1775
346 313.2209 312.2136
347 313.2390 312.2317
348 315.0977 314.0904
349 315.1828 314.1755
350 316.2129 315.2056
351 316.9379 315.9306
352 316.9380 315.9307
353 316.9382 315.9309
354 316.9383 315.9310
355 317.0770 316.0697
356 317.2103 316.2030
357 319.1317 318.1244
358 319.1386 318.1313
359 319.1525 318.1452
360 319.1525 318.1452
361 319.1527 318.1454
362 319.1528 318.1455
363 319.1528 318.1455
364 319.1528 318.1455
365 319.1530 318.1457
366 319.1530 318.1457
367 319.1531 318.1458
368 319.1532 318.1459
369 319.1534 318.1461
370 319.1534 318.1461
371 319.1895 318.1822
372 319.1896 318.1823
373 319.1897 318.1824
374 319.2259 318.2186
375 319.2261 318.2188
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Not what you expected? Try the select summary.