MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
76 305.0110 304.0037
77 305.0110 304.0037
78 305.0111 304.0038
79 305.0111 304.0038
80 305.0112 304.0039
81 305.0112 304.0039
82 305.0112 304.0039
83 305.0112 304.0039
84 305.0112 304.0039
85 305.0112 304.0039
86 305.0114 304.0041
87 305.0114 304.0041
88 305.0114 304.0041
89 305.0114 304.0041
90 305.0115 304.0042
91 305.0115 304.0042
92 305.0116 304.0043
93 305.0116 304.0043
94 305.0117 304.0044
95 305.0194 304.0121
96 305.0199 304.0126
97 305.1375 304.1302
98 305.1581 304.1508
99 305.1586 304.1513
100 305.1737 304.1664
101 305.1737 304.1664
102 305.1738 304.1665
103 305.1738 304.1665
104 305.1738 304.1665
105 305.1738 304.1665
106 305.1739 304.1666
107 305.1739 304.1666
108 305.1739 304.1666
109 305.1739 304.1666
110 305.1739 304.1666
111 305.1740 304.1667
112 305.1740 304.1667
113 305.1740 304.1667
114 305.1741 304.1668
115 305.1741 304.1668
116 305.1741 304.1668
117 305.1741 304.1668
118 305.1741 304.1668
119 305.1742 304.1669
120 305.1742 304.1669
121 305.2105 304.2032
122 305.5133 304.5060
123 307.0434 306.0361
124 307.0434 306.0361
125 307.0435 306.0362
126 307.0436 306.0363
127 307.0438 306.0365
128 307.1527 306.1454
129 307.1528 306.1455
130 307.1528 306.1455
131 307.1529 306.1456
132 307.1529 306.1456
133 307.1529 306.1456
134 307.1530 306.1457
135 307.1530 306.1457
136 307.1530 306.1457
137 307.1530 306.1457
138 307.1531 306.1458
139 307.1532 306.1459
140 307.1533 306.1460
141 307.1895 306.1822
142 307.1895 306.1822
143 307.1897 306.1824
144 307.1898 306.1825
145 307.1898 306.1825
146 307.1898 306.1825
147 307.1898 306.1825
148 307.1899 306.1826
149 307.1900 306.1827
150 307.1900 306.1827
151 307.1900 306.1827
152 307.1900 306.1827
153 307.1900 306.1827
154 307.1900 306.1827
155 307.1901 306.1828
156 307.1901 306.1828
157 307.1901 306.1828
158 307.1901 306.1828
159 307.1901 306.1828
160 307.2097 306.2024
161 307.2263 306.2190
162 308.0899 307.0826
163 308.0900 307.0827
164 308.0901 307.0828
165 308.0902 307.0829
166 308.0904 307.0831
167 308.0904 307.0831
168 308.0905 307.0832
169 308.0905 307.0832
170 308.0905 307.0832
171 308.0905 307.0832
172 308.0905 307.0832
173 308.0905 307.0832
174 308.0905 307.0832
175 308.0906 307.0833
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