MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3871 617.6389 3699.7897
3872 741.1639 3700.7831
3873 617.9708 3701.7811
3874 617.9711 3701.7829
3875 617.9714 3701.7847
3876 617.9716 3701.7859
3877 741.3646 3701.7866
3878 741.3657 3701.7921
3879 618.1332 3702.7555
3880 618.1353 3702.7681
3881 624.4741 3740.8009
3882 624.4766 3740.8159
3883 624.6385 3741.7873
3884 749.3650 3741.7886
3885 624.6404 3741.7987
3886 749.3672 3741.7996
3887 749.3677 3741.8021
3888 624.6415 3741.8053
3889 624.6422 3741.8095
3890 624.6430 3741.8143
3891 624.6433 3741.8161
3892 624.6437 3741.8185
3893 624.8023 3742.7701
3895 749.5679 3742.8031
3896 624.8087 3742.8085
3897 624.8104 3742.8187
3898 625.1381 3744.7849
3900 759.9716 3794.8216
3901 759.9725 3794.8261
3902 633.4843 3794.8621
3903 634.3113 3799.8241
3904 761.1705 3800.8161
3905 762.7776 3808.8516
3906 547.4135 3824.8436
3907 547.4138 3824.8457
3908 547.4144 3824.8499
3909 547.5577 3825.8530
3912 769.3725 3841.8261
3913 961.4645 3841.8289
3914 961.4648 3841.8301
3915 549.8419 3841.8424
3916 769.3774 3841.8506
3918 549.8435 3841.8536
3919 641.3171 3841.8589
3920 769.3792 3841.8596
3921 769.3794 3841.8606
3926 641.4816 3842.8459
3927 641.4822 3842.8495
3928 769.5781 3842.8541
3929 769.5781 3842.8541
3930 641.4830 3842.8543
3931 769.5787 3842.8571
3932 641.4836 3842.8579
3933 549.9871 3842.8588
3935 641.4838 3842.8591
3937 769.5792 3842.8596
3938 641.4839 3842.8597
3939 769.5793 3842.8601
3943 641.4851 3842.8669
3944 769.5818 3842.8726
3945 550.1300 3843.8591
3946 769.7792 3843.8596
3947 550.1301 3843.8598
3948 769.7793 3843.8601
3949 641.6507 3843.8605
3952 643.9811 3857.8429
3953 643.9825 3857.8513
3954 772.5787 3857.8571
3955 643.9835 3857.8573
3956 772.5795 3857.8611
3959 644.1503 3858.8581
3960 644.1508 3858.8611
3961 644.3157 3859.8505
3963 650.6541 3897.8809
3964 975.7191 3898.8473
3965 650.8220 3898.8883
3966 558.1320 3899.8731
3967 558.1331 3899.8808
3968 650.9875 3899.8813
3969 558.1332 3899.8815
3970 650.9878 3899.8831
3971 650.9887 3899.8885
3972 650.9888 3899.8891
3973 650.9888 3899.8891
3974 650.9889 3899.8897
3975 650.9892 3899.8915
3976 650.9907 3899.9005
3977 558.2755 3900.8776
3981 558.2768 3900.8867
3982 558.2776 3900.8923
3983 651.1560 3900.8923
3984 781.3583 3901.7551
3985 977.4891 3905.9273
3987 783.7669 3913.7981
3988 979.7039 3914.7865
3990 981.4370 3921.7189
3992 981.7170 3922.8389
3994 785.7708 3923.8176
3997 982.2202 3924.8517
3999 988.7133 3950.8241
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