MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3747 809.4222 3233.6597
3748 649.5094 3242.5106
3749 649.7092 3243.5096
3750 652.9095 3259.5111
3751 653.1108 3260.5176
3752 653.3082 3261.5046
3753 653.3104 3261.5156
3754 653.5145 3262.5361
3755 1095.6613 3283.9621
3756 658.9109 3289.5181
3757 1101.1730 3300.4972
3758 1101.5048 3301.4926
3759 835.4497 3337.7697
3761 837.4286 3345.6853
3763 838.1781 3348.6833
3764 670.9290 3349.6086
3765 670.9304 3349.6156
3766 1122.2618 3363.7636
3767 676.3076 3376.5016
3769 684.5247 3417.5871
3771 855.8980 3419.5629
3772 570.9368 3419.5771
3773 684.9253 3419.5901
3775 1152.1082 3453.3028
3776 696.1257 3475.5921
3777 581.2886 3481.6879
3778 1162.9146 3485.7220
3779 699.1241 3490.5841
3780 873.6545 3490.5889
3782 874.9918 3495.9381
3783 700.9214 3499.5706
3784 706.5436 3527.6816
3785 588.9545 3527.6833
3786 588.9549 3527.6857
3787 706.5445 3527.6861
3788 588.9550 3527.6863
3791 706.7442 3528.6846
3793 589.1217 3528.6865
3794 706.7460 3528.6936
3796 706.9450 3529.6886
3797 887.6613 3546.6161
3798 710.3315 3546.6211
3799 598.6252 3585.7075
3801 598.7935 3586.7173
3802 598.9599 3587.7157
3803 606.9713 3635.7841
3804 607.1385 3636.7873
3805 607.1387 3636.7885
3806 607.2876 3637.6819
3807 607.2983 3637.7461
3808 607.3047 3637.7845
3809 607.3065 3637.7953
3810 607.6362 3639.7735
3811 731.9404 3654.6656
3812 731.9417 3654.6721
3813 732.1394 3655.6606
3814 732.1414 3655.6706
3815 732.5333 3657.6301
3816 524.6899 3665.7784
3817 524.6901 3665.7798
3818 524.8329 3666.7794
3820 524.8347 3666.7920
3821 917.9149 3667.6305
3822 524.9755 3667.7776
3823 524.9760 3667.7811
3825 919.9753 3675.8721
3826 526.9745 3681.7706
3827 527.1169 3682.7674
3828 921.7140 3682.8269
3829 921.9528 3683.7821
3831 737.7653 3683.7901
3832 527.2632 3683.7915
3833 614.9727 3683.7925
3834 614.9730 3683.7943
3835 615.1351 3684.7669
3836 737.9609 3684.7681
3840 737.9641 3684.7841
3841 527.4051 3684.7848
3842 527.4052 3684.7855
3843 737.9644 3684.7856
3844 615.1384 3684.7867
3845 527.4056 3684.7883
3847 615.1387 3684.7885
3848 737.9650 3684.7886
3849 615.1390 3684.7903
3850 737.9658 3684.7926
3852 615.1394 3684.7927
3855 737.9664 3684.7956
3856 615.1400 3684.7963
3857 615.1401 3684.7969
3860 527.5459 3685.7704
3861 738.1642 3685.7846
3862 615.3055 3685.7893
3863 615.3067 3685.7965
3864 738.1671 3685.7991
3865 527.5502 3685.8005
3866 922.4574 3685.8005
3867 738.3599 3686.7631
3868 738.3611 3686.7691
3870 617.6375 3699.7813
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