MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4447)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4022 1003.2035 4008.7849 4008.8476 -15.6 1 1 0.92 +1Score > 20 indicates identity
Score > 13 indicates homology
SPTDLVDSMPSPIWNQYATGSYYDQIPAPPSPSNRR + 2 Deamidated (NQ)
2373 479.7899 957.5652 957.5607 4.75 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
QLNLVSRK + Deamidated (NQ)
2824 528.9136 1583.7190 1583.6885 19.2 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
MQNELRNGNYQSK + 3 Deamidated (NQ)
2695 364.7029 1454.7825 1454.7664 11.1 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
QQIDPRLAEMVR
2927 414.4536 1653.7853 1653.8079 -13.7 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
MASNAAMLLDPRAHR + Deamidated (NQ)
3917 769.3777 3841.8521 3841.8075 11.6 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
QGLQNQMFSIFMLMTIFGNLVQQIMPNFVTQR + 4 Deamidated (NQ); 4 Oxidation (M)
3252 677.3600 2029.0582 2029.0367 10.6 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
VKLVQGDLNNPAGIFENAK + 3 Deamidated (NQ)
4280 801.4232 5602.9115 5602.8544 10.2 1 1 1.3 +1Score > 15 indicates identity QDQLENAHLQIGTRIATAVNGIQNTTVADGTPFGLVQHLLSPITDALHPVGTR + 5 Deamidated (NQ)
2632 465.5653 1393.6741 1393.6473 19.2 0 1 1 +1Score > 24 indicates identity
Score > 14 indicates homology
ENGGLEGYQLASR + Deamidated (NQ)
4053 853.5919 4262.9231 4262.9678 -10.5 0 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
NMQILNQFGEYTACGIDSGHGFDIEYGTGNIPGLGVVFR + Deamidated (NQ); Oxidation (M)
4171 779.6649 5450.6034 5450.6243 -3.84 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
MSIPLEWRPTLSFSHETPDPNVSMHSVMLRLNYHLCMTIIHQASSR + 2 Oxidation (M)
2495 577.2861 1152.5576 1152.5775 -17.2 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
QLEHDIQAAK + Deamidated (NQ)
2804 522.2932 1563.8578 1563.8331 15.8 0 1 3.4 +1Score > 19 indicates identity SLFVLAQCVEAISK
3472 590.0275 2356.0809 2356.1196 -16.4 1 1 0.85 +1Score > 23 indicates identity
Score > 13 indicates homology
ASYFHDAASRDLLSALHDPDR
3400 753.0005 2255.9797 2255.9615 8.04 0 1 0.8 +1Score > 20 indicates identity
Score > 13 indicates homology
AHQLENMIYDHCYQYIR + 3 Deamidated (NQ)
3553 815.6760 2444.0062 2444.0083 -0.87 0 1 1.6 +1Score > 16 indicates identity EEHMDSFVPWTAIDMTSCTR + 2 Oxidation (M)
4259 799.4219 5588.9024 5588.9163 -2.50 1 1 1.5 +1Score > 15 indicates identity DYLILVAQLETQLVNNPSFTLHLLHLHTMPTSQCLAQLYSLGQELLRR + 2 Deamidated (NQ)
2651 473.9136 1418.7190 1418.7194 -0.30 1 1 0.88 +1Score > 24 indicates identity
Score > 13 indicates homology
KQGIKPNDGLWY + Deamidated (NQ)
3458 467.8564 2334.2456 2334.2293 6.97 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
FLSHPGIPTPTMEIIQLAQPK + Deamidated (NQ); Oxidation (M)
4071 892.2714 4456.3206 4456.2663 12.2 1 1 2.7 +1Score > 18 indicates identity SVPLPGLPTGPVQPEASQEPEAHQVAPSPPEQPRSPTPTPPVR + Deamidated (NQ)
3962 773.1739 3860.8331 3860.7583 19.4 0 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
ILIAIHDGQNLGIAMSGFMEYSEGSQDLWDMLTR + 2 Deamidated (NQ); 3 Oxidation (M)
3161 471.9756 1883.8733 1883.8757 -1.28 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
QNVMDYSLMIAIDESR
3940 641.4847 3842.8645 3842.9182 -14.0 1 1 0.94 +1Score > 23 indicates identity
Score > 13 indicates homology
LSTVQNADAIIVMKDGQILNHGTHSELMVTSALYR + 2 Deamidated (NQ); Oxidation (M)
4170 779.6642 5450.5985 5450.5329 12.0 0 1 0.83 +1Score > 21 indicates identity
Score > 13 indicates homology
FLVQQTHMFQFLGQPLLNETQGMNALGFHPSHYLDWTDYELPPESR + 3 Deamidated (NQ); Oxidation (M)
3795 589.2869 3529.6777 3529.6548 6.51 1 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
AGMLSTGAAWGIDQGMWAFRTHSPSDFFTQIK + Oxidation (M)
4063 871.2015 4350.9711 4350.9987 -6.34 1 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
SGYTGGVSDSSDSRIDQDINGTETVQGLSTADIGGLIYGEVSK + 3 Deamidated (NQ)
4262 799.5644 5589.8999 5589.8853 2.61 1 1 1.5 +1Score > 15 indicates identity LIPDLITTASVETVQAFLLFGVYTMPIDTSGLAYTYLGLAIKMAIQNGMHR + Deamidated (NQ); 2 Oxidation (M)
3731 1009.8673 3026.5801 3026.5250 18.2 0 1 1 +1Score > 20 indicates identity
Score > 14 indicates homology
LAPENPFPAALHDAEDAVNWVLQRPEK
3598 523.0378 2610.1526 2610.1438 3.37 1 1 1 +1Score > 21 indicates identity
Score > 14 indicates homology
NESSRTNTAPGEMIQGMNGASWVR + 2 Deamidated (NQ); Oxidation (M)
2224 829.3922 828.3849 828.3977 -15.5 0 1 3.8 +1Score > 19 indicates identity TLEHDSK
2744 502.5960 1504.7662 1504.7681 -1.29 1 1 0.85 +1Score > 24 indicates identity
Score > 13 indicates homology
HLVGADGAHSMVRR
4156 779.3779 5448.5944 5448.4934 18.5 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
EQSIDNVLYTLCADNQIRIWAMMEHHSPTVLQLWTQIDMGASVQPR + 6 Deamidated (NQ); 3 Oxidation (M)
4092 913.2763 4561.3451 4561.2952 11.0 0 1 3.7 +1Score > 19 indicates identity DGYQSNLHITKPGNSTGANPVVVLIFGGAFVMGTNIQSIIWAR + 3 Deamidated (NQ); Oxidation (M)
2591 443.9023 1328.6851 1328.6626 16.9 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
FNPERWLGPGR + Deamidated (NQ)
3118 456.9785 1823.8849 1823.8788 3.32 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
ESAEVLKEQGIQYSDK + Deamidated (NQ)
4032 1007.9703 4027.8521 4027.7817 17.5 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
CGVCTFWLGSPDPMSKACLAADAGQQAGKPTQAEMMR + Deamidated (NQ)
3414 454.6342 2268.1346 2268.1313 1.45 1 1 0.96 +1Score > 23 indicates identity
Score > 13 indicates homology
QLYDWLLKNNYADANLIAK + 3 Deamidated (NQ)
4069 743.7261 4456.3129 4456.3425 -6.64 1 1 3.2 +1Score > 18 indicates identity MIEALPLELLEMIIQIALDLDEQHKPTGVPKVWEAYPGR + Deamidated (NQ)
3995 785.7743 3923.8351 3923.8822 -12.0 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
SEDPIVRSNAVITLGDMAVCFNHLIDENTDFLYR
2957 556.9355 1667.7847 1667.7937 -5.40 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
QPEPSAEIPSGPRMR + Deamidated (NQ); Oxidation (M)
3520 597.7684 2387.0445 2387.0376 2.90 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
IHQASGINYEDMLFFDDEAR + Deamidated (NQ); Oxidation (M)
4029 670.8327 4018.9525 4018.9768 -6.04 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
VRLDQAQTIEELMGGIQQQALDMTPFEQFGLQNIR + 2 Deamidated (NQ)
4137 899.6029 5391.5737 5391.5021 13.3 1 1 0.85 +1Score > 20 indicates identity
Score > 13 indicates homology
SGDVQTLQFAWALQHLSERFWSSQSLNQTFLNNATNASQANYEPNFK + 4 Deamidated (NQ)
2126 363.1790 724.3434 724.3425 1.29 1 1 3.5 +1Score > 19 indicates identity MSKESK + Oxidation (M)
3824 524.9764 3667.7839 3667.7961 -3.32 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
IPRPPSGVYHHPLGQDADQHAQKWFEFLGDPK + Deamidated (NQ)
4276 1121.5876 5602.9016 5602.9823 -14.4 1 1 1.5 +1Score > 15 indicates identity NGLIFVLGGIFGIIVAAFFANQQDVISLDALMDLNLDALMDVIPQGIVKDVR + 2 Oxidation (M)
2684 726.8582 1451.7018 1451.7303 -19.6 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
GPGVEGAPAAPRAMR + Oxidation (M)
3957 772.7769 3858.8481 3858.8482 -0.030 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
ITHAAQSASGSQFAIANGMNQIYLFDSIASGSTRVSR + 3 Deamidated (NQ)
4347 808.8461 5654.8718 5654.7918 14.1 1 1 2.9 +1Score > 18 indicates identity VSQTWYIGGEGSTDVNDQQVGVVWPPGRSDNLLISLSLGGNLNYLAEGTPEPR
3222 985.9767 1969.9388 1969.9779 -19.8 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
MPTLADAVTAVAGPGGELQR + Deamidated (NQ); Oxidation (M)
3694 550.6714 2748.3206 2748.3640 -15.8 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
QLSITDDQTVIHESLLQVYMDKR + Deamidated (NQ); Oxidation (M)
2887 408.4267 1629.6777 1629.6619 9.67 0 1 2.5 +1Score > 17 indicates identity TPTTDSLCGGMGMMR + Oxidation (M)
3037 578.2496 1731.7270 1731.7588 -18.4 0 1 2.9 +1Score > 18 indicates identity SPWNEGNEVETLGGDK + Deamidated (NQ)
3191 387.7968 1933.9476 1933.9282 10.0 0 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
ASASATNFPPLGQPHGPQR + 2 Deamidated (NQ)
2637 702.8354 1403.6562 1403.6642 -5.70 0 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
LALMSFSQSFQK + 2 Deamidated (NQ); Oxidation (M)
2815 525.9146 1574.7220 1574.7260 -2.55 0 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
VQNCWLGPSNSTGR
3311 528.5236 2110.0653 2110.0596 2.70 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
DGVLPFSSWVSKVHNGQPR + Deamidated (NQ)
3732 1009.8679 3026.5819 3026.5495 10.7 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
VHEALQALTDEELPAFITSRCIGITSR
3356 738.3729 2212.0969 2212.0695 12.4 1 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
WNQLSHGTKALIQNCDIGR + 2 Deamidated (NQ)
2218 412.2447 822.4748 822.4712 4.46 0 1 2.8 +1Score > 18 indicates identity VIHQLGR + Deamidated (NQ)
2769 769.3842 1536.7538 1536.7321 14.2 0 1 1 +1Score > 24 indicates identity
Score > 13 indicates homology
SQSEHHLPGAPYSK
3998 790.3632 3946.7796 3946.8275 -12.1 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
TRGDNVYMMVELSHAIVDGGSTEVVLQEMSLAFDGK + Deamidated (NQ); 3 Oxidation (M)
2304 894.4258 893.4185 893.4315 -14.5 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
QSRSSSSR
3184 644.6075 1930.8007 1930.7963 2.27 0 1 2.7 +1Score > 17 indicates identity QDVGPIDSNSSASCEAHR + 2 Deamidated (NQ)
2371 478.7632 955.5118 955.5087 3.34 1 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
QPANQAKAK + Deamidated (NQ)
2186 797.3777 796.3704 796.3684 2.59 1 1 4 +1Score > 19 indicates identity MSTRMR + Oxidation (M)
3993 785.5770 3922.8486 3922.9128 -16.4 1 1 1 +1Score > 22 indicates identity
Score > 13 indicates homology
TNHEDALLVMSGTMGNQVAIRTHLVQPPYSVICDR + Deamidated (NQ)
2595 449.2458 1344.7156 1344.7051 7.78 1 1 1 +1Score > 23 indicates identity
Score > 13 indicates homology
SWTGVWQRVAR
2911 549.9174 1646.7304 1646.7497 -11.8 0 1 1 +1Score > 21 indicates identity
Score > 13 indicates homology
SIPEMQWAQVAEQK + 3 Deamidated (NQ)
3380 744.4117 2230.2133 2230.1858 12.3 1 1 4.4 +1Score > 19 indicates identity SFLFLHNKAQAENSGITVVR
4403 921.2744 6441.8699 6441.9584 -13.7 1 0 2.2 +1Score > 16 indicates identity GLAWYATYWLITASFVQATCYIYSPTHTMAPSWKGHLQQAVDCLESLGSSHDMAFR + 3 Deamidated (NQ); Oxidation (M)
2187 797.3799 796.3726 796.3789 -7.91 0 0 4.3 +1Score > 19 indicates identity MVFQQK + Deamidated (NQ); Oxidation (M)
2463 367.8904 1100.6494 1100.6706 -19.3 1 0 3.8 +1Score > 19 indicates identity IPRIVYVNK
4039 810.5547 4047.7371 4047.7531 -3.95 0 0 2.9 +1Score > 18 indicates identity SAQPTPMPMMPECQTFSPEELQTQPSMGYMSPAFAK + Oxidation (M)
4456 1114.8207 7796.6940 7796.6095 10.8 1 0 2.3 +1Score > 17 indicates identity MKPEPGVSEAGDQPVLAYPPHAPLGQVPNMHPDMRYQPQTHPNPALPLLQNPYMPGGYTSAPPMPNGGAPQGR + 7 Deamidated (NQ); 2 Oxidation (M)
4090 761.2313 4561.3441 4561.2952 10.7 0 0 4.1 +1Score > 19 indicates identity DGYQSNLHITKPGNSTGANPVVVLIFGGAFVMGTNIQSIIWAR + 3 Deamidated (NQ); Oxidation (M)
4415 944.0106 6601.0233 6600.9011 18.5 1 0 2.3 +1Score > 17 indicates identity AENLMCYIGHEGTYTPAHQEMCASLGQNIMVEASDGSVEYGQATKPGSSIWFMTESKDR + 2 Oxidation (M)
3079 593.6216 1777.8430 1777.8134 16.7 0 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
YTFQAFWEQAMALR + Deamidated (NQ); Oxidation (M)
3986 978.7092 3910.8077 3910.8710 -16.2 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
QPRHGPNYNQLLHLLNDMQNHSAAWPFTQPVNR + 2 Deamidated (NQ); Oxidation (M)
3230 496.4946 1981.9493 1981.9633 -7.06 0 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
EVAVPEAVLTSTGFEYDR
3292 527.5533 2106.1841 2106.1797 2.11 1 0 2.2 +1Score > 16 indicates identity IQSPPTLLLLENTGNALRR + Deamidated (NQ)
3362 445.0336 2220.1316 2220.1215 4.55 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
GWTVELVSFSQVTSYAYRK
3374 557.0096 2224.0093 2224.0187 -4.24 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
RECETCIMAYTHVGTQLR
4075 901.8715 4504.3211 4504.3774 -12.5 1 0 2.8 +1Score > 17 indicates identity ITTLSTQVAPPTHDLTRQIPTIQLLQAALIIEMLQFGQER + 4 Deamidated (NQ); Oxidation (M)
3338 731.0310 2190.0712 2190.1103 -17.9 1 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
VGIADTVGCASPRQVYELVR + Deamidated (NQ)
4328 806.5665 5638.9146 5638.8289 15.2 1 0 2 +1Score > 16 indicates identity NTPLYGNIIPDLLMAPLTCLFGLDIDNFSSPDGLLAKTELNLPVLGEEMHR + 2 Deamidated (NQ)
3485 473.4273 2362.1001 2362.1224 -9.42 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VIDVGNSMTPPHLHVSGDGETGK + Oxidation (M)
4250 798.5605 5582.8726 5582.9309 -10.4 1 0 2.7 +1Score > 17 indicates identity KPVPNDLIPEYEALLLAHEAYMAKFSERPTITLDLGIIPGLHTVAVNCPR + Deamidated (NQ)
3599 436.1982 2611.1455 2611.1773 -12.2 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
LDQAVQLSEQQQGPDHEDTLETK + 3 Deamidated (NQ)
3508 794.3774 2380.1104 2380.1515 -17.3 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
VTSLPIMVDGDTGYGSPMNVRR + Oxidation (M)
3854 615.1398 3684.7951 3684.8273 -8.71 0 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
ELLPSIMQPVLEAEDTENWQTLYYALQLYAK + Oxidation (M)
4237 929.1536 5568.8779 5568.8492 5.16 1 0 1.8 +1Score > 15 indicates identity TNASLAAGILNIAATFAAACASFLEDQRSIRPSDLLVIYLTMVAMCAIAPLR + 2 Deamidated (NQ); Oxidation (M)
4292 802.1015 5607.6596 5607.6571 0.44 0 0 1 +1Score > 20 indicates identity
Score > 13 indicates homology
AQMAISQAGQANSQMQQQLTQQSPAMPMLNRPMAPGQMSPAQVTAQVRPPSR + 2 Deamidated (NQ); 2 Oxidation (M)
4272 934.6578 5601.9031 5601.8618 7.39 0 0 1.6 +1Score > 15 indicates identity DLSAGETIVEASIQAFGPNIDILVNNAGVEVVKPLSDLTVEDYNLVYDLNVR + Deamidated (NQ)
2435 527.7251 1053.4356 1053.4549 -18.3 0 0 2.5 +1Score > 17 indicates identity EGHMGPPDSK
3317 706.3205 2115.9397 2115.9385 0.56 0 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology
NFIDYEEYPQSAEIQNR + Deamidated (NQ)
3978 781.1833 3900.8801 3900.8161 16.4 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
REEQADTVTYWYMMNGGQQIVGGLLAYCFGLIGK + Deamidated (NQ); 2 Oxidation (M)
3790 588.9559 3527.6917 3527.7024 -3.03 1 0 1 +1Score > 23 indicates identity
Score > 13 indicates homology
QSMGSEQDLLHIQNPMRLLPGDGESLDLVYR + Deamidated (NQ); Oxidation (M)
2638 468.9070 1403.6992 1403.6715 19.7 0 0 1 +1Score > 24 indicates identity
Score > 13 indicates homology
ASLGTGVANGMDPAK + Oxidation (M)
4351 808.9944 5655.9099 5655.9685 -10.4 1 0 2.3 +1Score > 16 indicates identity ALTVDGGGIRGIVSLQILLTLQNLLGPHCPLPDLIDVAFGTSAGMSPPQPPPSPR + 3 Deamidated (NQ)
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