MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1231_JBEI_1.mgf
Database : A-oryzae 20191108 (12,205 sequences; 5,465,702 residues)
Timestamp : 1 May 2025 at 20:38:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,476

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 21 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4447)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3634 667.0750 2664.2709
3635 667.0750 2664.2709
3636 533.8632 2664.2796
3637 534.2277 2666.1021
3638 534.2299 2666.1131
3639 667.5992 2666.3677
3640 667.6001 2666.3713
3641 535.8629 2674.2781
3642 895.7625 2684.2657
3643 896.0618 2685.1636
3644 538.0440 2685.1836
3645 897.2662 2688.7768
3646 539.2277 2691.1021
3647 898.1042 2691.2908
3648 673.8307 2691.2937
3649 539.2661 2691.2941
3650 673.8311 2691.2953
3651 898.1061 2691.2965
3652 673.8316 2691.2973
3653 539.2668 2691.2976
3654 673.8318 2691.2981
3655 673.8320 2691.2989
3656 539.2671 2691.2991
3657 898.1070 2691.2992
3658 539.2672 2691.2996
3659 673.8325 2691.3009
3661 539.4274 2692.1006
3662 539.4283 2692.1051
3663 674.0817 2692.2977
3664 898.4404 2692.2994
3665 539.4673 2692.3001
3666 539.4675 2692.3011
3667 899.4633 2695.3681
3668 544.8658 2719.2926
3670 545.0630 2720.2786
3671 681.0782 2720.2837
3672 545.0669 2720.2981
3673 908.8477 2723.5213
3675 546.8561 2729.2441
3676 683.3187 2729.2457
3677 683.3193 2729.2481
3678 546.8569 2729.2481
3680 547.0585 2730.2561
3681 912.1007 2733.2803
3682 684.3323 2733.3001
3683 912.1174 2733.3304
3684 684.8354 2735.3125
3685 914.4374 2740.2904
3686 914.6144 2740.8214
3687 915.8392 2744.4958
3688 916.7031 2747.0875
3689 688.0866 2748.3173
3690 688.0869 2748.3185
3691 688.0872 2748.3197
3692 550.6713 2748.3201
3693 688.0874 2748.3205
3695 550.6719 2748.3231
3697 917.1165 2748.3277
3698 917.4446 2749.3120
3699 688.3365 2749.3169
3700 550.8720 2749.3236
3701 917.4493 2749.3261
3702 918.2780 2751.8122
3703 923.4869 2767.4389
3704 702.5852 2806.3117
3705 562.4720 2807.3236
3706 702.8400 2807.3309
3707 702.8404 2807.3325
3708 562.4745 2807.3361
3709 562.6732 2808.3296
3710 937.7734 2810.2984
3711 947.9921 2840.9545
3712 949.8563 2846.5471
3713 952.4636 2854.3690
3714 578.2498 2886.2126
3715 722.5630 2886.2229
3716 967.2870 2898.8392
3717 729.6342 2914.5077
3718 972.5104 2914.5094
3719 729.6362 2914.5157
3720 977.1610 2928.4612
3721 734.5616 2934.2173
3722 979.0815 2934.2227
3723 987.8605 2960.5597
3725 593.4729 2962.3281
3726 992.4749 2974.4029
3727 603.4999 3012.4631
3728 603.5005 3012.4661
3729 603.5005 3012.4661
3730 1008.4711 3022.3915
3734 1015.0225 3042.0457
3735 766.6517 3062.5777
3736 616.4796 3077.3616
3738 1041.1677 3120.4813
3739 635.1142 3170.5346
3740 635.5162 3172.5446
3742 795.1656 3176.6333
3743 795.3644 3177.4285
3745 645.1179 3220.5531
3746 645.3170 3221.5486
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